Luciferase Control RNA from Promega

Product Specs
ItemLuciferase Control RNA
CompanyPromega
Price $72.00Supplier Page View Company Product Page
Catalog NumberL4561
Quantity20microg

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Translating the blueprint of cell fate: eIF5A-mediated translation regulates cellular identity in the pancreasMol MetabApril 2, 2026Danielle L. Overton, Catharina B P Villaca, Dorian J. Dale, Caleb D. Rutan, Morgan A. Robertson, Craig T. Connors, Emily K. Anderson-Baucum, Teresa L. MastracciLuciferase (catalog #: Mr03987587_mr). Gene expression was normalized to the spiked-in Luciferase (1 μg/μL, L4561, Promega). The mRNA abundance was graphed as a relative expression. Statistical significance was determined

(2) Expression of free fatty acid receptors in the liver of periparturient dairy cows supplemented with essential fatty acids and conjugated linoleic acidJDS CommunicationsMarch 1, 2026Tainara C. Michelotti, Alyssa Imbert, Arash Veshkini, Guillaume Durand, Harald M. Hammon, Muriel Bonnetno. 4387406, Applied Biosystems) and 2 pg/µL of luciferase encoding reporter transcript (catalog no. L4561, Promega). Expression of FFAR1, FFAR2, FFAR3, GPR84, and PPARD were determined by real-time quantitative PCR

(3) Environmental Amino Acid Sensing Regulates the Rate of ASC Translation and NLRP3 Inflammasome AssemblybioRxivJanuary 23, 2026Mikel D. Haggadone, Brian P. Goldspiel, Aoife O’Farrell, Nora T. Kiledjian, Montana Knight, Talia R. Smith, Elena Anderson, Víctor R. Vázquez Marrero, Mark A. Boyer, Peining Jimmy Xu, et al.of TRIzol LS (ThermoFisher Scientific #10296028) containing 20 pg Luciferase control mRNA (Promega #L4561) was added and thoroughly mixed followed by addition of 1 mL 100% ethanol. Samples were then loaded

(4) The RNA-binding protein PRRC2B preserves 5' TOP mRNA during starvation to maintain ribosome biogenesis during nutrient recoveryNucleic Acids ResDecember 11, 2025Nadav Goldberg, Doron Bril, Miriam Eisenstein, Tsviya Olender, Alon Savidor, Shani Bialik, Shmuel Pietrokovski, Adi Kimchimanufacturer’s instructions. The lysate was spiked with 10 ng Firefly luciferase mRNA (Promega, cat# L4561) for every 100 000 cells. 750 ng total RNA was transcribed into cDNA using AzuraQuant™ II cDNA Synthesis

(5) Single-base m 6 A epitranscriptomics reveals novel HIV-1 host interaction targets in primary CD4 + T cellsJournal of VirologyNovember 25, 2025Siyu Huang, Yutao Zhao, Stacia Phillips, Julia E. Warrick, Michael G. Kearse, Chuan He, Li Wucollected from the sucrose gradient were divided into 10 large fractions; 0.2 ng Luciferase Control RNA (L4561, Promega) was added to 400 µL of each fraction. The RNA was extracted using TRIzol. The levels of HIV-1

(6) Decapping activators Edc3 and Scd6 act redundantly with Dhh1 in post-transcriptional repression of starvation-induced pathwayseLifeNovember 25, 2025Rakesh Kumar, Fan Zhang, Shreyas Niphadkar, Chisom Onu, Anil Kumar Vijjamarri, Miriam L Greenberg, Sunil Laxman, Alan G Hinnebuschor kit,DNase I,Roche,Cat # 4716728001, Commercial assay or kit,Luciferase Control RNA,Promega,Cat # L4561, Commercial assay or kit,Superscript III First-Strand synthesis kit,Invitrogen,Cat # 18080051, Commercial

(7) Caloric Restriction Enhances the Efficacy of Anti-Androgen Therapy in Prostate Cancer by Inhibiting Androgen Receptor TranslationCancer ResearchNovember 3, 2025Ricardo A. Cordova, May Elbanna, Christopher Rupert, Sabrina A. Orsi, Noah R. Sommers, Angela J. Klunk, Li Shen, Jagannath Misra, Jocelyne N. Hanquier, Maria Tsompana, et al.were spiked with 10 ng/µl exogenous polyadenylated Luciferase spike-in mRNA control (Promega, Cat. #L4561). RNA from fractions was isolated using TRIzol LS Reagent (ThermoFisher, Cat. #10296028) according to

(8) A dual-mechanism antimicrobial peptide with antimutagenic activity targets the replisome and induces cell envelope stressmSphereSeptember 30, 2025Amanda Holstad Singleton, Olaug Elisabeth Torheim Bergum, Jana Scheffold, Synnøve Brandt Ræder, Lilja Brekke Thorfinnsdottir, Lisa Marie Røst, Caroline Krogh Søgaard, Per Bruheim, Marit Otterleithe 25 µL reactions were carried out in Eppendorf tubes with 1 µL luciferase control RNA (Promega, L4561) and incubated at 37°C for 1 h. After stopping the reaction for 5 min in an ice bath, 25 µL of Luciferase Assay Reagent

(9) The many ways to inhibit translation by Sorafenib in liver cancer cellsMolecular and Cellular BiochemistrySeptember 15, 2025Laura Contreras, Sara Ricciardi, Stefano Biffo, Jordi Muntané, Jesús de la Cruzaccording to the experimental design. Before RNA extraction, 0.5 µg of a commercial luciferase RNA (cat. L4561, Promega) in a volume of 2 µl was added to each pool to normalise for RNA recovery. A treatment with

(10) Maternal plasma cell-free RNA as a predictive test for fetal lung maturationBMC MedJuly 28, 2025Sean W. D. Carter, Kay Yi Michelle Seah, Si En Poh, Winston Koh, Haruo Usuda, Erin L. Johnson, Yusaku Kumagai, Tsukasa Takahashi, Lara J. Monteiro, Reyna Peñailillo, et al.pooled reverse primer mix in the presence of 105 copies of luciferase control RNA (Promega, Cat no. L4561) and a final concentration 2 mM of dNTPs at 65°C for 5 min. Reverse transcription of cfRNA was performed

(11) The Integrated Stress Response Pathway Coordinates Translational Control of Multiple Immune Checkpoints in Lung CancerCancer ResearchJuly 15, 2025Shayna Thomas-Jardin, Shruthy Suresh, Ariana Arce, Nicole Novaresi, Qing Deng, Emily Stein, Lisa Thomas, Cheryl Lewis, Chul Ahn, Bret M. Evers, et al.isolate RNA and cDNA synthesis, and RT-PCR was performed. Twenty ng of luciferase mRNA control (Promega, L4561) was added to each fraction prior to RNA extraction to control for variability in total RNA in fractions

(12) G-quadruplex topologies determine the functional outcome of guanine-rich bioactive oligonucleotidesNucleic Acids ResJune 28, 2025Prakash Kharel, Nupur Bhatter, Safiyah Zubair, Shawn M Lyons, Paul J Anderson, Pavel Ivanovpreparation The following reporter mRNAs were used in this study. Uncapped FL mRNA (Promega®, Cat# L4561) was directly used from the kit without further modification. Capped FL mRNA was prepared using Cellscript

(13) Predictive Screening for Inflammatory Disorders of Pregnancy Using Targeted Maternal Cell-Free RNA Assays: Proof-of-Principle Data from Large Animal and Human CohortsReproductive SciencesJune 4, 2025Sean W. D. Carter, Qin Wei, Winston Koh, Xiawen Liu, Kay Yi Michelle Seah, Si En Poh, Haruo Usuda, Erin L. Fee, Yusaku Kumagai, Tsukasa Takahashi, et al.pooled reverse primer mix in the presence of 105 copies of luciferase control RNA (Promega, Cat no. L4561) and a final concentration 2 mM of dNTPs at 65 °C for 5 min. Reverse transcription of cfRNA was performed

(14) Sorafenib-associated translation reprogramming in hepatocellular carcinoma cellsRNA BiolMarch 21, 2025Laura Contreras, Alfonso Rodríguez-Gil, Jordi Muntané, Jesús de la Cruzin V1 and V2 samples and before RNA extraction, 0.5 µg of a commercial luciferase RNA control (cat. L4561, Promega, Madison, WI, USA) in a volume of 2 µl was added to each one. Note that, in addition to using

(15) A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformationMolecular CellMarch 6, 2025Raja H. Ali, Esteban A. Orellana, Su Hyun Lee, Yun-Cheol Chae, Yantao Chen, Jim Clauwaert, Alyssa L. Kennedy, Ashley E. Gutierrez, David J. Papke, Mateo Valenzuela, et al.(L4960, Promega) 40 units of RNasin (N2111, Promega), 2 mM MgCl2 and 2 μl of luciferase control mRNA (L4561, Promega) preheated to 70°c for three minutes. Where indicated, 1 μg of recombinant METTL1 or active

(16) METTL3 alters capping enzyme expression and its activity on ribosomal proteinsScientific ReportsNovember 12, 2024Daniel del Valle-Morales, Giulia Romano, Giovanni Nigita, Michela Saviana, Alessandro La Ferlita, Patricia Le, Rachel Brown, Lavender Micalo, Howard Li, Patrick Nana-Sinkam, et al.Culjkovic-Kraljacic et al.15. 4 µg of total RNA spiked with 1 ng/µL of Luciferase Control RNA (Promega #L4561) as a negative uncapped control. 10% of RNA and spike-in was taken as input. Bound RNA was purified

(17) Liver cancer initiation requires translational activation by an oncofetal regulon involving LIN28 proteinsThe Journal of clinical investigationJune 13, 2024Hsieh MH, Wei Y, Li L, Nguyen LH, Lin YH, Yong JM, Sun X, Wang X, Luo X, Knutson SK, et al.RNA; then cDNA synthesis and RT-qPCR were performed, and 20 ng of Luciferase mRNA control (Promega, L4561) was added to each fraction prior to RNA extraction to control for variability in total RNA in fractions

(18) The Development of a One-Step RT-qPCR for the Detection and Quantification of Viable Forms of Trypanosoma cruzi in Açai Samples from Areas at Risk of Chagas Disease through Oral TransmissionInternational Journal of Molecular SciencesMay 18, 2024Amanda Faier-Pereira, Paula Finamore-Araujo, Carlos Ramon do Nascimento Brito, Eldrinei Gomes Peres, Klenicy Kazumy de Lima Yamaguchi, Daniele Pereira de Castro, Otacilio C. Moreira[19]" RNA-IAC (VIC/NFQ-MGB),Forward,5′-TAC AAC ACC CCA ACA TCT TCG A-3′,Not Available,"Promega (Cat. Number: L4561), Johnson et al., 2005 [35]" RNA-IAC (VIC/NFQ-MGB),Not Available,"Promega (Cat. Number: L4561), Johnson

(19) Mechanisms of antiviral action and toxicities of ipecac alkaloids: Emetine and dehydroemetine exhibit anti-coronaviral activities at non-cardiotoxic concentrationsVirus ResearchMarch 1, 2024Viktoriya S. Sidorenko, Ira Cohen, Kunchok Dorjee, Conceição A. Minetti, David P. Remeta, Junyuan Gao, Irina Potapova, Hong Zhan Wang, Janet Hearing, Wan-Yi Yen, et al.reactions (20 μl) contained 80 % TNT Quick master mix, 20 µM methionine, 40 ng/µL luciferase RNA (Promega; L4561), and test chemicals. Samples were incubated at 30 °C for 90 min and diluted 10-fold with PBS. An aliquot

(20) Targeting eIF4A triggers an interferon response to synergize with chemotherapy and suppress triple-negative breast cancerThe Journal of clinical investigationDecember 15, 2023Zhao N, Kabotyanski EB, Saltzman AB, Malovannaya A, Yuan X, Reineke LC, Lieu N, Gao Y, Pedroza DA, Calderon SJ, et al.hundred fifty microliters of each fraction was mixed with 500 pg of luciferase RNA spike-in (Promega, L4561) and lysed in 750 μL TRIzol LS (Thermo Fisher Scientific, 10296028). RNA was precipitated in the presence

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