Wheat Germ Extract from Promega

Product Specs
ItemWheat Germ Extract
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL4380
Quantity5 x 200microl
SystemWheat Germ Extract
Molecular Weight~74kDa
SpeciesWheat

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systemsbioRxivMarch 30, 2026Philipp Koch, Frederic S. W. Arendrup, Chaehee Lim, Samyukta Narayanan, Amina Adam, Massimiliano Clamer, Anders H. Lund, Chun-Kan Chen, Kathrin Leppekgenerated and purified circRNA was used throughout all in vitro translation systems. For the Promega WGL (L4380) and RRL (L4960) extracts, 10–500ng of linear Fluc mRNA and Nluc-encoding circRNA were used at a ratio

(2) The basic helix-loop-helix (bHLH) transcription factor DTT1 is part of a paired key that unlocks the tapetum transition in barley anther developmentThe Plant CellOctober 8, 2025Miaoyuan Hua, Wenzhe Yin, Alison C Tidy, José Fernández Fernández Gómez, Huanjun Li, Shuya Shi, Guangwei Xing, Jie Zong, Zoe A WilsonpF3A-Kozak-3xHA-HvDTT1, were generated for protein expression with TNT wheat germ cell-free protein system (Promega, L4380). The empty pF3A-Kozak-3xHA vector was used as the negative control. The anti E-box artificial dsDNA

(3) Liposomal lipid nanoparticles for extrahepatic delivery of mRNANat CommunMay 3, 2025Miffy Hok Yan Cheng, Yao Zhang, Kevin Fox, Jerry Leung, Colton Strong, Emma Kang, Yihang Chen, Michelle Tong, Hemashree Bommadevara, Eric Jan, et al.per well. After confirmation of RNA quality, a wheat germ extract assay was performed (Promega Cat#L4380) following the standard protocol. Following the wheat germ extract assay, the samples were diluted by

(4) Reversible 2′-OH acylation enhances RNA stabilityNature ChemistrySeptember 1, 2023Linglan Fang, Lu Xiao, Yong Woong Jun, Yoshiyuki Onishi, Eric T. Kool384-well microplate (Greiner Bio-One, 781900). To it was added 12.5 μl of wheat germ extract (Promega, L4380), 0.5 μl of RNasin Plus RNase inhibitor (Promega, N2611), 2.0 μl of Complete Amino Acid mix (Promega,

(5) Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteinsGenome researchAugust 1, 2023Avolio R, Agliarulo I, Criscuolo D, Sarnataro D, Auriemma M, De Lella S, Pennacchio S, Calice G, Ng MY, Giorgi C, et al.synthesize eGFP and the RTS 100 E. coli HY kit used to synthesize EmGFP were purchased from Promega (L4380) and Biotechrabbit (BR1400102), respectively. eGFP mRNA has been transcribed in vitro using a pLEXY-eGFP

(6) TP53BP1, a dual-coding gene, uses promoter switching and translational reinitiation to express a smORF proteiniScienceMay 19, 2023Inchingolo MA, Diman A, Adamczewski M, Humphreys T, Jaquier-Gubler P, Curran JA.commercial assays,Critical commercial assays,Critical commercial assays ,, Wheat germ extracts,Promega,#L4380 Dual-Luciferase Reporter Assay System,Promega,#E1910 WesternBright™ Quantum,Advansta,#K-12042-D10 ,, Experimental models

(7) Cytosolic and mitochondrial translation elongation are coordinated through the molecular chaperone TRAP1 for the synthesis and import of mitochondrial proteinsbioRxiv : the preprint server for biologyJanuary 19, 2023Avolio R, Agliarulo I, Criscuolo D, Sarnataro D, Auriemma M, Pennacchio S, Calice G, Ng MY, Giorgi C, Pinton P, et al.and the RTS 100 E. coli HY Kit used to synthesize EmGFP mRNA were purchased from Promega (Cat. No. L4380) and Biotechrabbit (Cat. No. BR1400102), respectively. For the Wheat Germ translation assay, we assembled

(8) OsMADS58 Stabilizes Gene Regulatory Circuits during Rice Stamen DevelopmentPlants (Basel)October 28, 2022Liping Shen, Feng Tian, Zhukuan Cheng, Qiang Zhao, Qi Feng, Yan Zhao, Bin Han, Yuhan Fang, Yanan Lin, Rui Chen, et al.(Life Technologies, Carlsbad, CA, USA, #AM1330) and Wheat Germ Extract Systems (Promega, Madison, WI, USA, #L4380), according to the manufacturer’s instructions. After testing protein quality, the protein samples were

(9) N1-methylpseudouridine found within COVID-19 mRNA vaccines produces faithful protein productsCell ReportsAugust 30, 2022Kyusik Q. Kim et al.assays ,, Dual-Luciferase Reporter Assay System,Promega,Cat#E1910 Wheat Germ Extract,Promega,Cat#L4380 ,, Deposited data,Deposited data,Deposited data ,, Mass Spectrometry Data,This Study,ProteomeXchange:

(10) PABP/purine-rich motif as an initiation module for cap-independent translation in pattern-triggered immunityCellAugust 18, 2022Wang J, Zhang X, Greene GH, Xu G, Dong X.Assays Dual Luciferase Reporter Assay System,Promega,Cat. #E1910 Wheat Germ Extract,Promega,Cat. #L4380 RiboMAX™ Large Scale RNA Production Systems,Promega,Cat. #P1300 Vaccinia Capping System,NEB,Cat. #M2080S Experimental Models

(11) Effects of the Noncoding Subgenomic RNA of Red Clover Necrotic Mosaic Virus in Virus InfectionJournal of VirologyFebruary 9, 2022Pulkit Kanodia, W. Allen Millerbedgraph file was plotted in the Fig. 12. In vitro translation. Wheat germ extract (WGE; Promega, L4380) was used for in vitro translation. Triplicates of 389 ng of each (25 nM final RNA concentration) of

(12) ZFP207 sustains pluripotency by coordinating OCT4 stability, alternative splicing and RNA exportEMBO reportsFebruary 3, 2022Malla S, Prasad Bhattarai D, Groza P, Melguizo-Sanchis D, Atanasoai I, Martinez-Gamero C, Román ÁC, Zhu D, Lee DF, Kutter C, et al.(MegaScript T7 Transcription kit, Thermo Fisher #AM1333) and in vitro translated (Wheat Germ in vitro translation kit, Promega #L4380). The in vitro‐translated ZFP207‐Halo fusion product was incubated with total RNA from mouse ESCs. Prior

(13) A Helicase Unwinds Hexanucleotide Repeat RNA G-Quadruplexes and Facilitates Repeat-Associated Non-AUG TranslationJournal of the American Chemical SocietyMay 19, 2021Liu H, Lu YN, Paul T, Periz G, Banco MT, Ferré-D'Amaré AR, Rothstein JD, Hayes LR, Myong S, Wang J.polyacrylamide gel electrophoresis. In vitro translation assays were performed using a wheat germ extract kit (Promega, L4380). Briefly, reactions (50 μl) were carried out in 96-well black plates (Costar) containing 25 μl wheat

(14) Mayahuelin, a Type I Ribosome Inactivating Protein: Characterization, Evolution, and Utilization in Phylogenetic Analyses of AgaveFrontiers in Plant ScienceMay 27, 2020Fernando Lledías, Jesús Gutiérrez, Aída Martínez-Hernández, Abisaí García-Mendoza, Eric Sosa, Felipe Hernández-Bermúdez, Tzvetanka D. Dinkova, Sandi Reyes, Gladys I. Cassab, Jorge Nieto-Sotelowas tested using luciferase as a reporter on a cell-free wheat germ protein synthesis system (cat. L4380, Promega). Mayahuelin was tested at different nM concentrations and, as a positive control, saporin

(15) An in vitro single-molecule assay for eukaryotic cap-dependent translation initiation kineticsNucleic Acids ResearchNovember 13, 2019Hongyun Wang, Lexi Sun, Anthony Gaba, Xiaohui QuRRL translation mixtures for single-molecule detection Wheat germ extract (WGE) was from Promega (L4380). Translation reaction mixtures for single-molecule detection were assembled according to the manufacturer's

(16) Multisite Phosphorylation of S6K1 Directs a Kinase Phospho-code that Determines Substrate SelectionMolecular cellFebruary 7, 2019Arif A, Jia J, Willard B, Li X, Fox PL.,Life Technologies,Cat# LC6070 Fugene 6 transfection reagent,Promega,Cat# E2691 Wheat germ extract system,Promega,Cat# L4380 6xHis Fusion Protein Spin Purification Kit (Ni- affinity chromatography),ThermoFisher (Pierce),Cat#

(17) A novel role for sox7 in Xenopus early primordial germ cell development: mining the PGC transcriptomeDevelopment (Cambridge, England)January 8, 2018Butler AM, Owens DA, Wang L, King ML.presence of pou5f3.3-MO and sox7-MO, respectively, was tested using the Wheat Germ Extract Kit (Promega, L4380) as per the manufacturer’s protocol. Translation of sox7-FL rescue, pou5f3.3-FL and pou5f3.3-FL rescue

(18) RISC-interacting clearing 3’- 5’ exoribonucleases (RICEs) degrade uridylated cleavage fragments to maintain functional RISC in Arabidopsis thalianaeLifeMay 2, 2017Zhonghui Zhang, Fuqu Hu, Min Woo Sung, Chang Shu, Claudia Castillo-González, Hisashi Koiwa, Guiliang Tang, Martin Dickman, Pingwei Li, Xiuren ZhangTNT T7 Quick Coupled transcription/translation system (Promega, L1170), mixed together (1:1, v:v) with 1 vol fresh wheat germ extract (Promega, L4380) and incubated for 30 min at 25°C in the presence of additional 0.75 mM ATP, 1.8 mM MgCl2, 20 mM creatine

(19) Noncanonical Expression of a Murine Cytomegalovirus Early Protein CD8 T-Cell Epitope as an Immediate Early Epitope Based on Transcription from an Upstream GeneVirusesFebruary 14, 2014Annette Fink, Julia K. Büttner, Doris Thomas, Rafaela Holtappels, Matthias J. Reddehase, Niels A. W. Lemmermannof the in vitro transcripts was performed with the Promega wheat germ extract (Promega, Catalog No. L4380, Mannheim, Germany). The reaction conditions were 90 min at 25 °C with 80 ng of template RNA. The resulting

(20) The extracellular signal-regulated kinase-mitogen-activated protein kinase pathway phosphorylates and targets Cdc25A for SCF beta-TrCP-dependent degradation for cell cycle arrestMolecular biology of the cellApril 1, 2009Isoda M, Kanemori Y, Nakajo N, Uchida S, Yamashita K, Ueno H, Sagata N.2002). In Vitro Translation In vitro translation of Xe-Cdc25A mRNA was performed using wheat germ extracts (L4380; Promega, Madison, WI) as described previously (Inoue et al., 2007). GST Fusion Proteins cDNAs encoding

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