Rabbit Reticulocyte Lysate, Untreated from Promega

Product Specs
ItemRabbit Reticulocyte Lysate, Untreated
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL4151
Quantity1ml
SystemRabbit Reticulocyte
Molecular Weight~74kDa
SpeciesRabbit

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) The G3BP stress-granule proteins reinforce the integrated stress response translation programmeNature Cell BiologyJanuary 1, 2026Jarrett Smith, David P. BartelIn vitro translation The fLuc mRNA was in vitro translated using a rabbit reticulocyte lysate system (Promega, L4151) following the standard non-nuclease treated protocol, with the exception of using a shorter incubation

(2) HIV-1 nuclear import is selective and depends on both capsid elasticity and nuclear pore adaptabilityNature MicrobiologyJuly 7, 2025Zhen Hou, Yao Shen, Stanley Fronik, Juan Shen, Jiong Shi, Jialu Xu, Long Chen, Nathan Hardenbrook, Alan N. Engelman, Christopher Aiken, et al.was added to the 40 µl reaction, achieving a final concentration of 16% (v/v) RRL (catalogue number L4151; Promega), 0.6 mM ATP, 0.06 mM GTP, 5 mM creatine phosphate and 10 U µl−1 creatine kinase. The reaction

(3) Correlative In Situ Cryo-ET Reveals Cellular and Viral Remodeling Associated with Selective HIV-1 Core Nuclear ImportbioRxivMarch 4, 2025Zhen Hou, Yao Shen, Stanley Fronik, Juan Shen, Jiong Shi, Jialu Xu, Long Chen, Nathan Hardenbrook, Christopher Thompson, Sarah Neumann, et al.mix was added to the 40 μL reaction, achieving a final concentration of 16% (v/v) RRL (Promega, cat: L4151), 0.6 mM ATP, 0.06 mM GTP, 5 mM creatine phosphate, and 10 U/μL creatine kinase. The reaction was then

(4) TFIIH mutations can impact on translational fidelity of the ribosomeHuman Molecular GeneticsMarch 20, 2023Fatima Khalid, Tamara Phan, Mingyue Qiang, Pallab Maity, Theresa Lasser, Sebastian Wiese, Marianna Penzo, Marius Alupei, Donata Orioli, Karin Scharffetter-Kochanek, et al.performed according to the protocol of Penzo et al. (32). In brief, rabbit reticulocyte lysate (Promega L4151) was depleted from ribosomes after mixing with an equal volume of cold 3.2 mM MgCl2 solution and 40%;v

(5) P300 Interacted With N-Myc and Regulated Its Protein Stability via Altering Its Post-Translational Modifications in NeuroblastomaMolecular & Cellular ProteomicsMarch 1, 2023Cheng Cheng, Tian He, Kai Chen, Yuanxia Cai, Yaoyao Gu, Lijia Pan, Peiwen Duan, Yeming Wu, Zhixiang WuU5507; Sigma–Aldrich), 20 mM MG132, and 5 μl/reaction crude rabbit reticulocyte lysate (catalog no.: L4151; Promega) for 1 h at 30 °C (24). Products were then subjected to IP using GST antibody (catalog no.:

(6) eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translationNucleic acids researchFebruary 28, 2023Schmidt T, Dabrowska A, Waldron JA, Hodge K, Koulouras G, Gabrielsen M, Munro J, Tack DC, Harris G, McGhee E, et al.E2065S), HiScribe™ T7 mRNA Kit with CleanCap® Reagent AG (NEB E2080S), Rabbit Reticulocyte Lysate (Promega L4151), TMT 16plex reagent kit (A44522 Thermo Scientific). RNAs: all RNAs were purchased from IBA life sciences

(7) Phosphorylation of enteroviral 2A(pro) at Ser/Thr125 benefits its proteolytic activity and viral pathogenesisJournal of medical virologyJanuary 1, 2023Wang Y, Zou W, Niu Y, Wang S, Chen B, Xiong R, Zhang P, Luo Z, Wu Y, Fan C, et al.templates (p1300; Promega). Protein was overexpressed according to the instructions of the RRL System (L4151; Promega). 2.11 Infectious clone and rescued virus The plasmids pBR322‐EV‐A71 derived from the genomic

(8) Phosphorylation of SARS-CoV-2 Orf9b Regulates Its Targeting to Two Binding Sites in TOM70 and Recruitment of Hsp90International Journal of Molecular SciencesAugust 26, 2021Lukas Brandherm, Antonio Mario Kobaš, Mara Klöhn, Yannick Brüggemann, Stephanie Pfaender, Joachim Rassow, Sebastian Kreimendahlfollowed by centrifugation for 20 min at 20,000× g, and resuspended in untreated reticulocyte lysate (L4151; Promega, Madison, WI, USA). 4.4. In Vitro Binding Assay To analyze the binding of proteins to the

(9) The molecular basis of coupling between poly(A)-tail length and translational efficiencyeLifeJuly 2, 2021Xiang K, Bartel DP.35–45 mg/ml. In vitro translation Five hundred µl rabbit reticulocyte lysate obtained from Promega (L4151, untreated) was supplemented with 1 µl 12.5 mM Hemin (Sigma, 51280), 25 µl 1 M HEPES pH 7.5, and 2.5

(10) Mucoricin is a ricin-like toxin that is critical for the pathogenesis of mucormycosisNature microbiologyMarch 1, 2021Soliman SSM, Baldin C, Gu Y, Singh S, Gebremariam T, Swidergall M, Alqarihi A, Youssef EG, Alkhazraji S, Pikoulas A, et al.modification of a previously described method33. Briefly, a rabbit reticulocyte lysate (Promega, Cat: L4151) was thawed at 37°C immediately before use and supplemented with 40 μl of 1 mM hemin stock solution

(11) mRNA structural elements immediately upstream of the start codon dictate dependence upon eIF4A helicase activityGenome BiologyDecember 30, 2019Joseph A. Waldron, David C. Tack, Laura E. Ritchey, Sarah L. Gillen, Ania Wilczynska, Ernest Turro, Philip C. Bevilacqua, Sarah M. Assmann, Martin Bushell, John Le Quesneammonium acetate. For sequencing gels, 75 μl nuclease untreated Rabbit Reticulocyte Lysate (Promega L4151), supplemented with 25 μM haemin, 25 μg/ml creatine kinase, 3 mg/ml creatine phosphate, 50 μg/ml liver

(12) Heterologous Production and Functional Characterization of Ageritin, a Novel Type of Ribotoxin Highly Expressed during Fruiting of the Edible Mushroom Agrocybe aegeritaApplied and Environmental MicrobiologyNovember 1, 2019Annageldi Tayyrov, Sophie Azevedo, Robert Herzog, Eva Vogt, Simon Arzt, Peter Lüthy, Pie Müller, Martin Rühl, Florian Hennicke, Markus Künzlerassay. To detect rRNA cleavage activity, 20 ␮l of untreated rabbit reticulocyte lysate (product ID, L4151; Promega, WI, USA) was mixed with puriﬁed recombinant, wild-type, or mutant ageritin or its paralog

(13) Generation of ribosome imprinted polymers for sensitive detection of translational responsesScientific reportsJuly 26, 2017King HA, El-Sharif HF, Matia-González AM, Iadevaia V, Fowotade A, Reddy SM, Gerber AP.gel. In vitro translation assays The rabbit reticulocyte in vitro translation assay system (Promega #L4151) was used to monitor translation activity of purified human ribosomes. Reactions were assembled in triplicates

(14) A neomorphic cancer cell-specific role of MAGE-A4 in trans-lesion synthesisNature communicationsJuly 5, 2016Gao Y, Mutter-Rottmayer E, Greenwalt AM, Goldfarb D, Yan F, Yang Y, Martinez-Chacin RC, Pearce KH, Tateishi S, Major MB, et al.phosphokinase (FisherSci, ICN10050990), PBS and 1 mg ml−1 of rabbit reticulocyte lysate, untreated (L4151), from Promega, as a source of ubiquitination factors and proteasome activity, as described by Hernandez-Pigeon

(15) PCAF regulates the stability of the transcriptional regulator and cyclin-dependent kinase inhibitor p27 Kip1Nucleic acids researchAugust 1, 2012Pérez-Luna M, Aguasca M, Perearnau A, Serratosa J, Martínez-Balbas M, Jesús Pujol M, Bachs O.from Perkin Elmer. Histones and cycloheximide were from Sigma-Aldrich. Rabbit reticulocyte extracts (L4151) were from Promega. The following antibodies were used in this work: Acetyl-Lys (600-401-939), from

(16) Cytoplasmic Prep1 Interacts with 4EHP Inhibiting Hoxb4 TranslationPLoS ONEApril 13, 2009J. Carlos Villaescusa, Claudia Buratti, Dmitry Penkov, Lisa Mathiasen, Jesús Planagumà, Elisabetta Ferretti, Francesco Blasiextracted total RNA from fresh non treated lysate (Promega, Rabbit Reticulocyte Lysate, Untreated, cat. L4151). Degenerate primers for amplification of HoxA and HoxB cDNA were used as described previously [18],

(17) Facilitated nucleocytoplasmic shuttling of the Ran binding protein RanBP1Molecular and cellular biologyMay 1, 2000Plafker K, Macara IG.of creatine kinase/ml, 500 ␮M ATP, 500 ␮M GTP) and rabbit reticulocyte lysate (Promega catalog no. L4151) or HeLa cell lysate and/or purified recombinant factors, incubated at room temperature for 5 to 30

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