T7 RiboMAX Express Large Scale RNA Production System from Promega

Product Specs
ItemT7 RiboMAX Express Large Scale RNA Production System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberP1320
Quantity1 system

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Orthogonal CRISPR screens and human liver chimeric mice identify hepatitis B virus host factorsbioRxivJuly 24, 2026Catherine A Freije, Georgios Dangas, Antonis Athanasiadis, Evgenia Moschogianni, Madeleine K Sanders, Allan Henrique Depieri Depieri Cataneo, Chloe K Boehm, Maria Bousali, Dar-Yin Li, Sooyoung Lee, et al.was performed using 2 μg of linearized and purified plasmid as input and the RiboMax T7 kit (Promega #P1320) according to the manufacturer’s protocol. Reactions were incubated for 30 min at 37°C, followed by

(2) Endolysosomal two-pore channels regulate a conserved entry pathway for enterovirusesJ Gen VirolJuly 15, 2026Ta-Chou Weng, Bang-Yan Hsu, Tung-Heng Wu, Xin-Ya Gu, Hsu-Hung Cheng, Szu-Hao Kungtemplate for in vitro transcription with the RiboMAX™ Large Scale RNA Production System-T7 (Promega, P1320), as described previously [27]. The transcribed RNA was precipitated with LiCl and washed with ice-cold

(3) Targeting RANKL-independent osteoclastogenesis overcomes denosumab resistance in models of ER+ breast cancer bone metastasisJ Clin InvestMay 15, 2026Qun Lin, Jinpeng Luo, Zhuxi Duan, Jieer Luo, Wei Zhang, Yuan Xia, Yinduo Zeng, Xiaolin Fang, Jiahui Liang, Jiayi Chen, et al.with the T7 polymerase promoter using a RiboMax Express Large Scale RNA Production system (Promega, P1320). After PCR amplification, 1 μg T7-DNA fragments were incubated with 0.5 mM dNTPs and 2 μl T7 RNA polymerase

(4) Poliovirus 3Dpol polymerase region is essential for cleavage of poliovirus 3AB in vivoPLoS PathogMay 13, 2026Minetaro Aritatranscripts of PV replicons were obtained using a RiboMAX Express Large Scale RNA Production System (Promega, P1320) with DraI-linearized plasmids of PV replicons. RNA transcripts (0.025 μL) were transfected into the

(5) Microprotein Regulates G-quadruplex Driven RNA AggregationbioRxivApril 18, 2026Bikash R. Sahoo, Janakraj Bhattrai, Arnav Sharma, Ursula Jakob, James CA Bardwellreactions set up with the T7 RiboMAX™ Express Large Scale RNA Production system kit (Promega, Cat.# P1320), following the manufacturer’s instructions. Post-transcription, samples were treated with DNase I at

(6) LncRNA HOXC-AS3 prevents chondrocyte senescence and osteoarthritis progression through miR-615-3p sponging and RRBP1 interactionCell & BioscienceFebruary 15, 2026Chan Wang, Jun Zhou, Lulu Chen, Changyan Ma, Zhan Dong, Dengshun Miaoantisense RNA were synthesized using the T7 RiboMAX Express Large Scale RNA Production System (Promega, P1320) and Biotin RNA Labeling Mix (Roche, 11,685,597,910). Cell lysates were incubated with purified biotinylated

(7) The inflammasome next door: characterizing pyroptosis induction in HCV-infected and uninfected bystander cells in vitroFrontiers in Cellular and Infection MicrobiologyFebruary 3, 2026Hannah L. Wallace, Cassandra L. Gardner, Calvin N. Ezeanyaegbu, Jordan Wight, Andrew S. Lang, Rodney S. Russelland in vitro transcribed using the T7 RiboMAX™ Express large scale RNA production system (Promega, P1320) according to manufacturer instructions. RNA encoding either JFH1T or JFH1T ΔGDD (non-replicating RNA;

(8) Balanced DNA-to-cytoplasm ratio at the 2-cell stage is critical for mouse preimplantation developmentiScienceJanuary 16, 2026Tao Pan, Natsumi Taira, Miho Ohsugicommercial assays,Critical commercial assays ,, RiboMax Large Scale RNA Production System-T7,Promega,P1320 Click-iT™ RNA Alexa Fluor™ 488 Imaging Kit,Thermo Fisher Scientific,C10329 Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent

(9) TDP-43 directly inhibits mRNA accumulation in neurites through modulation of mRNA stabilityThe EMBO JournalDecember 15, 2025Charlie Moffatt, Ankita Arora, Katherine F Vaeth, Bryan B Guzman, Gurprit Bhardwaj, Audrey Hoelscher, Levi B Gifford, Holger A Russ, Daniel Dominguez, J Matthew TaliaferroBiolabs,#M0530 Qiagen Gel Extraction kit,Qiagen,#28704 High Capacity T7 In vitro transcription kit,Promega,#P1320 SUPERase IN,Invitrogen,#AM2694 Dynabeads MyOne Streptavidin T1,Invitrogen,#65602 magnetic stand,Invitrogen,#12321D Amicon

(10) Cepharanthine inhibits enterovirus entry by endolysosomal deacidification and exhibits protective activity in vivoAntimicrobial Agents and ChemotherapyDecember 10, 2025Ta-Chou Weng, Bang-Yan Hsu, Szu-Hao Kungutilized for in vitro transcription with the RiboMAX Large Scale RNA Production System-T7 (Promega, P1320). Following transcription, the RNA was precipitated by LiCl (Cat. #7447-41-8, Ambion AM9480) and washed

(11) SDS-22 stabilizes GSP-1/-2 PP1 subunits contributing to polarity establishment in C. elegans embryosEMBO ReportsNovember 6, 2025Yi Li, Ida Calvi, Monica GottaGateway® BP Clonase™ II Enzyme Mix,Invitrogen,11789-020 Proteinase K,Invitrogen,25530-015 T7 RiboMAX™ Express Large Scale RNA Production,Promega,P1320 Taq DNA polymerase,Roche,03734927001 Pfu DNA polymerase,Promega,M7745 HindIII,New England Biolabs,R0104S BsrBI,New England Biolabs

(12) Cancer-associated fibroblast-derived circKLHL24 drives perineural invasion in pancreatic cancer via dual regulation of the sec31a-CXCL12 axisJ Exp Clin Cancer ResOctober 7, 2025Tingting Li, Rihua He, Qing Tian, Tianhao Huang, Honghui Jiang, Huimou Chen, Yuan Yuan, Yong Jiang, Shangyou Zheng, Chonghui Hu, et al.Transcription was carried out using the RiboMax Express Large Scale RNA Production System (Promega, P1320). Each 20 µl reaction contained 1 µg DNA template, 0.5 mM dNTPs, and 2 µl T7 RNA polymerase, and was

(13) Manganese suppresses tumor growth through hyper-activating IRE1αiScienceAugust 15, 2025Ruoxi Shi, Ming Wang, Si Chen, Xinwei Hu, Xudong Zhao, Shixin Zhou, Congli Fan, Zihan Zhou, Likun WangProtein Assay Kit,Beyotime,Cat#P0012 T7 RiboMAX™ Express Large Scale RNA Production System,Promega,Cat#P1320 Nuclear and Cytoplasmic Protein Extraction Kit,Beyotime,Cat#P0028 Colorimetric TUNEL Apoptosis Assay

(14) A Strand-Specific Quantitative RT-PCR Method for Detecting vRNA, cRNA, and mRNA of H7N9 Avian Influenza Virus in a Mouse ModelVirusesJuly 17, 2025Bo Wang, Guangwen Wang, Yi-Han Wang, Xuwei Liu, Manman Li, Huihui Kong, Hualan Chen, Li Jiang, Chengjun Liin vitro from double-stranded DNA products using the RiboMax Large Scale RNA Production System (Cat. No. P1320; Promega, Madison, WI, USA) [20]. The RNAs transcribed in vitro were concentrated and purified using

(15) Lineage-specific head development in the coffin-headed cricket Loxoblemmus equestris links the final molt with novel trait evolutionEvodevoJuly 16, 2025Mizuho Yoneda, Shinichi Morita, Teruyuki Niimi, Takaaki Daimon, Takahiro OhdeRNA was transcribed from a DNA template using the T7 RiboMAX Express Large Scale RNA System (Promega, P1320) and purified using phenol/chloroform/isoamyl alcohol (125:24:1) (Merck, 77619-100ML). The transcribed

(16) Multiomics Integration of Parkinson''s Disease Datasets Reveals Unexpected Roles of IRE1 in Its PathologyInternational journal of molecular sciencesJuly 12, 2025Pasat BA, Moncan M, Pilalis E, Samali A, Chatziioannou A, Gorman AM.templates for in vitro transcription with the T7 RiboMAX Express Large Scale RNA Production System (P1320, Promega, Madison, WI, USA) following the manufacturer’s instructions. The RNA was purified with the

(17) Systematic analysis of nonsense variants uncovers peptide release rate as a novel modifier of nonsense-mediated mRNA decayCell GenomicsJuly 9, 2025Divya Kolakada, Rui Fu, Nikita Biziaev, Alexey Shuvalov, Mlana Lore, Amy E. Campbell, Michael A. Cortázar, Marcin P. Sajek, Jay R. Hesselberth, Neelanjan Mukherjee, et al.Templates were run-off transcribed with the T7 RiboMAX Large Scale RNA Production System (Promega, P1320) kit according to the manufacturer’s protocol. The mRNA was then purified sequentially by isolation

(18) Lysyl-tRNA synthetase orchestrates translation and hormone signaling during reproduction in Aedes aegypti mosquitoesCommun BiolJuly 2, 2025Shan Li, Xueli Wang, Haoran Lu, Alexander S. Raikhel, Zhen Zoutreatment The dsRNA was prepared using a T7 RiboMAX Express Large Scale RNA Production System (Promega, P1320). The coding region of EGFP was used to generate the control dsRNA. Females within 24 h PE were injected

(19) Contributions of Folded and Disordered Domains to RNA Binding by HNRNPRbioRxivMay 25, 2025Bryan B. Guzmán, Grant A. Goda, Alli Jimenez, Justin G. Martyr, Yue Hu, Francisco F. Cavazos, Maria M. Aleman, Daniel DominguezIn vitro transcription was performed using T7 RiboMAX Express Large Scale RNA Production System (Promega, #P1320) following the instruction by the manufacturer. stG4 RNA pool: stG4 pool was designed as previously

(20) Testis-specific serine/threonine kinase dTSSK2 regulates sperm motility and male fertility in DrosophilaCommun BiolMay 8, 2025Ju Peng, Angyang Sun, Jie Zheng, Na Zhang, Xuedi Zhang, Guanjun Gao(gRNAs) were synthesized using the RiboMAX Large Scale RNA Production Systems-T7 Kit (Promega, #PR-P1320). Cas9 mRNA and gene-specific gRNAs were then purified and mixed to achieve final concentrations of

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