Bacterial Strain JM109, Glycerol Stock from Promega

Product Specs
ItemBacterial Strain JM109, Glycerol Stock
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberP9751
Quantity500microl
Cell Type (standardized)Microbial Cells
Validated Species ReactivityEscherichia

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Assessing PARP trapping dynamics in ovarian cancer using a CRISPR-engineered FRET biosensorCell Reports MethodsJanuary 26, 2026Daniel Marks, Edwin Garcia, Sunil Kumar, Katie Tyson, Caroline Koch, Aleksandar P. Ivanov, Joshua B. Edel, Hasan B. Mirza, William Flanagan, Christopher Dunsby, et al.AB2827587 ,, Bacterial strains,Bacterial strains,Bacterial strains ,, JM109 competent cells,Promega,Cat# P9751 High competency StbI3,NEB,Cat# B90205 One Shot™ TOP10 Chemically Competent E. coli,Invitrogen,Cat#

(2) A new CRISPR‐mediated Apc knockout allele leads to pyloric gland adenoma‐like gastric polyps in mice with C57BL/6;FVB/N mixed backgroundAnimal Models and Experimental MedicineFebruary 16, 2025Sarp Uzun, Özge Özcan, Ayşenur Gök, Aynur Işık, Sinem Bakır, Ayşen Günel‐Özcan, İlyas Onbaşılar, Aytekin Akyolsite downstream of the human U6 promoter. The final px300 product was transformed into JM109 (Promega, P9751) competent cells and plated on LB agar plates supplemented with 50 μg/mL of ampicillin. Single colonies

(3) Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectorsCell ReportsAugust 27, 2024Magellan Tchouakui, Sulaiman S. Ibrahim, Mersimine K. Mangoua, Riccado F. Thiomela, Tatiane Assatse, Sonia L. Ngongang-Yipmo, Abdullahi Muhammad, Leon J.M. Mugenzi, Benjamin D. Menze, Themba Mzilahowa, et al.strains,Bacterial and virus strains ,, E. coli JM109 competent cells,"Promega, United Kingdom",Cat# P9751 ,, Biological samples,Biological samples,Biological samples ,, Mosquitoes RNA and cDNA,Our lab,NA Mosquitoes

(4) Development of Broad-Range Microbial Minimal Culture Medium for Lanthanide StudiesMicroorganismsJuly 26, 2024Gianmaria Oliva, Giovanni Vigliotta, Luca Di Stasio, Ermanno Vasca, Stefano CastiglionePromega Italia Srl, Milan, Italy (http://www.promega.com/products, accessed on 1 June 2024; cat. no P9751), while S. aureus was isolated from hospital patients as part of the same collection mentioned above.

(5) Prime editing‐mediated correction of the leptin receptor in muscle cells of db/db miceBiotechnology JournalMay 1, 2024Kyung Eun Lee, Yanping Xu, Bingchuan Geng, Jongsoo Kim, Natalie Kellon, Zhixian He, Zhentao Zhang, Deqiang Li, Doug A. Gouchoe, Hua Zhu°C, then hold at 12 °C. Transformation of competent E. coli cells (Promega, Bacterial Strain JM109, # P9751) with the ligation reaction was performed by the conventional calcium method. Three clones were picked

(6) Exogenous DNA enhances DUOX2 expression and function in human pancreatic cancer cells by activating the cGAS-STING signaling pathwayFree Radical Biology and MedicineAugust 1, 2023Stephen L. Wang, Yongzhong Wu, Mariam Konaté, Jiamo Lu, David Mallick, Smitha Antony, Jennifer L. Meitzler, Guojian Jiang, Iris Dahan, Agnes Juhasz, et al.included: pGL3-Basic Vector (pGL3-BV, E1751), bacterial strain JM109 for bacterial genomic DNA extraction (P9751) from Promega, pcDNA3.1-HA plasmid (pcDNA3,128034) from Addgene, and pReceiver-M08 plasmid (EX-NEG-M08)

(7) CRISPR Gene Editing of Virulent Bacteriophage ICP1Cold Spring Harb ProtocApril 28, 2023Andrew CamilliAntarctic Phosphatase Reaction Buffer (New England Biolabs B0289) Escherichia coli cloning strain JM109 (Promega P9751) Golden Gate Assembly Kit (BsaI-HF v2; New England Biolabs E1601) ICP1 DNA (Seed et al. 2011) Plasmid

(8) Direct activation of KCC2 arrests benzodiazepine refractory status epilepticus and limits the subsequent neuronal injury in miceCell reports. MedicineMarch 21, 2023Jarvis R, Josephine Ng SF, Nathanson AJ, Cardarelli RA, Abiraman K, Wade F, Evans-Strong A, Fernandez-Campa MP, Deeb TZ, Smalley JL, et al.and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, E. coli JM109,Promega,P9751 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(9) Disruption of RING and PHD Domains of TRIM28 Evokes Differentiation in Human iPSCsCellsJuly 29, 2021Sylwia Mazurek, Urszula Oleksiewicz, Patrycja Czerwińska, Joanna Wróblewska, Marta Klimczak, Maciej Wiznerowiczsequencing primers are listed in Table S2. All plasmid vectors were transformed into E. coli JM109 (#P9751, Promega, Madison, WI, USA Research) and isolated with JETSTAR 2.0 Plasmid Maxiprep Kit (#220100, Gentaur,

(10) Studies on Physiological Effects and Plasmid Stability of JM109 Host SystemJournal of Pharmaceutical Research InternationalNovember 13, 2020Praveen Kumar Vemuri, Shaik Mohammad Anjum, Lakshmi Saranya Medida, P. V. Hemanth Sai, Mahathi Gummavajjala, Braja Kishori Panigrahi, Swati Dash, Swati SenapatiMETHODS All molecular biology kits were procured from Thermo Fischer, India. E coli JM109 (Promega: P9751) was procured from Promega - India. All solvents and reagents were of analytical grade, and all experiments

(11) Retapamulin-Assisted Ribosome Profiling Reveals the Alternative Bacterial ProteomeMolecular cellMay 2, 2019Meydan S, Marks J, Klepacki D, Sharma V, Baranov PV, Firth AE, Margus T, Kefi A, Vázquez-Laslop N, Mankin AS.gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ(lac-proAB), [F´ traD36, proAB, laqIqZΔM15]) (Promega, #P9751). Metabolic labeling of proteins Inhibition of protein synthesis by RET was analyzed by metabolic

(12) Neutrophil Extracellular Traps Confine Pseudomonas aeruginosa Ocular Biofilms and Restrict Brain InvasionCell host & microbeApril 10, 2019Thanabalasuriar A, Scott BNV, Peiseler M, Willson ME, Zeng Z, Warrener P, Keller AE, Surewaard BGJ, Dozier EA, Korhonen JT, et al.GFP,Created in this study,NA PAO1 dexos GFP,Created in this study,NA E. coli JM109,Promega,Cat# (P9751) Experimental Models: Organisms/Strains,Experimental Models: Organisms/Strains,Experimental Models:

(13) A Critical Analysis of the Role of SNARE Protein SEC22B in Antigen Cross-PresentationCell reportsJune 27, 2017Wu SJ, Niknafs YS, Kim SH, Oravecz-Wilson K, Zajac C, Toubai T, Sun Y, Prasad J, Peltier D, Fujiwara H, et al.(EV, 10879) plasmid was obtained from Addgene. Plasmids were transformed into JM109 competent cells (P9751, Promega) following manufacturer’s instructions. Preps were purified for lentiviral generation using

(14) The yield and quality of cellular and bacterial DNA extracts from human oral rinse samples are variably affected by the cell lysis methodologyJournal of Microbiological MethodsMarch 1, 2016Mohsen Sohrabi, Raj G. Nair, Lakshman P. Samaranayake, Li Zhang, Abu Hasanat Md. Zulfiker, Adnan Ahmetagic, David Good, Ming Q. Weiserially T diluted DNA standards from the PCR products of Escherichia coli strain JM109 (Promega, IP P9751), Streptococcus australis (isolated from human saliva) and human U-937 cells SC R ™ were used for

(15) Nucleo-cytoplasmic shuttling dynamics of the transcriptional regulators XYR1 and CRE1 under conditions of cellulase and xylanase gene expression in Trichoderma reeseiMolecular microbiologyOctober 10, 2014Lichius A, Seidl-Seiboth V, Seiboth B, Kubicek CP.strains used and generated in this study are listed in Table S1. Escherichia coli strains JM109 (cat.no. P9751, Promega, Madison, Wisconsin), One Shot®Top10 (cat.no. C4040-10, Life Technologies-Invitrogen, Austria)

(16) A new method to determine initial viability of entrapped cells using fluorescent nucleic acid stainingBioresource TechnologySeptember 8, 2010Tanush Wadhawan, Zachary B. Maruska, Sumana Siripattanakul, Christopher B. Hill, Anindita Gupta, Birgit M. Prüß, John M. McEvoy, Eakalak Khan

(17) Assessing tetrazolium and ATP assays for rapid in situ viability quantification of bacterial cells entrapped in hydrogel beadsEnzyme and Microbial TechnologyMay 11, 2010Tanush Wadhawan, John McEvoy, Birgit M. Prüß, Eakalak Khan

(18) HHV-8 ELISA based on a one-step affinity capture of biotinylated K8.1 antigenJournal of Virological MethodsFebruary 16, 2001Attila Juhász, József Kónya, Zoltán Beck, Éva Remenyik, György Veress, Ágnes Bégány, Ildikó Medgyessy, János Hunyadi, Lajos Gergely

Be the First to Write a Review!