HindIII from New England Biolabs

Product Specs
ItemHindIII
CompanyNew England Biolabs
Price $66.00 Inquire
Catalog NumberR0104S
Quantity10000 units
Concentration20-100 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

A/AGCTT

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Emergence and evolution of chimeric pseudogenes (φgenes) contribute to genetic and functional diversity of the human genomeNucleic Acids ResApril 28, 2026Amruta R Jadhav, Sushmita Sahoo, Yash L Kuber, Krutika Hatwar, Avinash Varanwal, Khushi Tanwar, Shubhankar Palwankar, Gauri Chatrasal, Avinash M Mali, Sharmila A BapatRMND5A and ANAPC1 sequences into the pGL3 vector, digestion was performed using KpnI and HindIII (NEB, #R0104S). For overexpression cloning of vA-202, the pLKO.CMV was used. To clone protein ORF into the p3xFlag

(2) Human respiratory syncytial virus regulates the expression of interferon-stimulated genes through modulation of fibrillarinFront Cell Infect MicrobiolApril 15, 2026José Manuel Ulloa-Aguilar, Victor Javier Cruz-Holguin, Yazmín Rocío Benítez-Zeferino, Edgar Rodrigo Guzmán-Bautista, Julio García-Cordero, Luis Adrián De Jesús-González, Julio Angel Vázquez-Martínez, Edgar Ricardo Vázquez-Martínez, Luis Herrera-Moro Huitron, Alfredo Mosqueda-Gracida, et al.with the restriction enzymes BglII (New England Biolabs, #R0144S) and HindIII (New England Biolabs, #R0104S). Following digestion, the plasmid vector was dephosphorylated using alkaline phosphatase (Thermo Fisher Scientific

(3) Optimized ND4 allotopic expression for gene therapy of Leber’s hereditary optic neuropathyFrontiers in Bioengineering and BiotechnologyMarch 20, 2026Evgeniy V. Lapshin, Alexander D. Egorov, Alexander S. Malogolovkin, Nizami B. Gasanov, Svetlana A. Smirnikhina, Vyacheslav Yu Tabakov, Alexander V. Karabelskymanufacturer of pAAV-CMV-MCS using BamHI and HindIII restriction endonucleases (NEB, cat. R0136S and R0104S). The ligation reaction of the prepared inserts and vectors was performed according to the manufacturer’s

(4) DPEP2 suppresses hyperinflammation via metabolic reprogramming of macrophages in sepsisNature CommunicationsMarch 9, 2026Wenchen Luo, Wei Xu, Qimeng Yin, Jieqiong Song, Jie Wang, Yian Guan, Jian Huang, Jian Yu, Kefeng Zou, Danfeng Jin, et al.TakaraBio). The recombinant plasmid overexpressing Dpep2 was treated with the Hind III restriction enzyme (R0104S, New England Biolabs) at 37 °C for 1 h, and the linearized template plasmid was purified using a PCR

(5) Nascent transcriptome of embryonic genome activation reveals a regulatory axis linking transcriptional priming to early lineage specification in mouse embryosNucleic Acids ResearchFebruary 27, 2026Yue Hu, Yuxiang Wang, Maosheng Ye, Yuanlin He, Zhangyi Ouyang, Chao Ren, Tianyin Miao, Siqi Wang, Ou Zhong, Li Liu, et al.vector with a GFP-tag and SP6 promoter, linearized with HindIII restriction enzyme (New England Biolabs, R0104S), and transcribed with MEGAscript Kit (Thermo Fisher Scientific, AM1330). To generate a membrane-anchored

(6) Effects of topological domain disruption on transcriptional regulation are chromatin context dependentEpigenetics & ChromatinFebruary 1, 2026Ángel Josué Cerecedo-Castillo, Diana Itzé Mojica-Santamaría, Hober Nelson Núñez-Martínez, Carlos Alberto Peralta-Alvarez, Gustavo Tapia-Urzúa, Georgina Guerrero, Rodrigo Gacel Arzate-Mejía, Félix Recillas-Targaprecipitated, and the second digestion was performed overnight with 50 U of NlaIII (NEB R0125S) or HindIII (NEB R0104S). The second ligation was performed in 5 mL total with 3000 U of T4 DNA ligase (NEB M0202L). The second

(7) Engineering T3 and T7 host range to target protein receptors and guide bacterial evolutioniScienceJanuary 16, 2026Collins Ogari, Kevin Yehlrecombinant proteins" ,, EcoRI,New England Biolabs,Cat# R3101S HindIII,New England Biolabs,Cat# R0104S Polyethylene Glycol (PEG) MW:8000,MilliporeSigma,Cat#25322-68-3 SYBRTM Safe,ThermoFisher,Cat#2687600 Agar,Fisher

(8) Nuclear cell-free DNA on the loose: an early warning signal of ischemia–reperfusion injury in kidney transplantationFrontiers in ImmunologyJanuary 7, 2026Gabriel Strandberg, Rebecca Trattner, Myriam Martin, Carl M. Öberg, Carl Raihle, Oleg Slivca, Shahnawaz Alam, Mårten Segelmark, Anders Christensson, Bo Nilsson, et al.nuclear DNA (#10031244; Bio-Rad, Hercules, California, United States), restriction enzyme HindIII (# R0104S; New England Biolabs, Ipswich, Massachusetts, United States) mixed with NE buffer r2.1 (#B6002S; New England Biolabs

(9) Epromoters bind key stress-related transcription factors to regulate clusters of stress response genesThe EMBO JournalJanuary 3, 2026Juliette Malfait, Jing Wan, Himanshu Narayan Singh, Charbel Souaid, Gaëlle Farah, Junhua Su, Magali Torres, Iris Manosalva, Nathalie Sakakini, Cyril Esnault, et al.,New England Biolabs,R3136S SalI-HF,New England Biolabs,R3138S BglII,New England Biolabs,R0144S HindIII,New England Biolabs,R0104S Alt-R CRISPR-Cas9 System,Integrated DNA Technologies,075915 Alt-R HDR Enhancer V2,Integrated DNA Technologies,10007910 Alt-R™-s.p Cas9 Nuclease V3

(10) Stress-induced toxic genomic R-loops support biofilm extracellular matrix formationNat CommunDecember 12, 2025Sudarsan Mugunthan, Zhang Dong, Sanjay H. Chotirmall, Staffan Kjelleberg, Thomas Seviourunits of each of the following: BsrGI (cat. No. R0575S), EcoRI (cat. No. R0101S), HindIII (cat. No. R0104S), SspI (cat. No. R0132S), XbaI (cat. No. R0145S) at 37 °C for 1 h66. This was followed by immunoprecipitation

(11) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce Dempleconfirmed the sequence. To eliminate an extra Nt.BspQI site located outside the N3 region, HindIII (NEB, R0104S) and AflIII (NEB, R0541S) enzymes were employed. Using 30 units each of HindIII and AflIII, 10 μg of

(12) Rbm8a deficiency causes hematopoietic defects by modulating Wnt/PCP signalingDevelopmental BiologyDecember 1, 2025Agnese Kocere, Elena Chiavacci, Charlotte Soneson, Seth T. Jacobson, Emma N. Harrison, Kevin Manuel Méndez-Acevedo, Jacalyn S. MacGowan, Harrison H. Wells, Max S. Hiltabidle, Azhwar Raghunath, et al.5′-AAAAATAATACGACTCACTATAGGGCCTTCACACTAGTGTGGG-3′, T7 polymerase for IVT); runx1 (using pBS-runx1a, linearized with HindIII (R0104S, NEB), T7 polymerase for IVT) (North et al., 2007) (a kind gift from Dr. Teresa V. Bowman); gfi1aa (using

(13) SDS-22 stabilizes GSP-1/-2 PP1 subunits contributing to polarity establishment in C. elegans embryosEMBO ReportsNovember 6, 2025Yi Li, Ida Calvi, Monica GottaProduction,Promega,P1320 Taq DNA polymerase,Roche,03734927001 Pfu DNA polymerase,Promega,M7745 HindIII,New England Biolabs,R0104S BsrBI,New England Biolabs,R0102S PrimeScript Reverse Transcription Kit,Takara,RR037B FastStart Universal

(14) FGF21 confers neuroprotection in Parkinson's disease by activating the FGFR1-sirt1 pathwayCell Mol Biol LettOctober 28, 2025Tingting Liu, Jianshe Weifragments were ligated into the pcDNA3.1-mCherry-C2 vector (with restriction enzyme sites HindIII-XbaI) (R0104S; R0145S; NEB, Beijing, China), followed by screening of positive clone strains with 100% accurate sequences.

(15) The mTORC2 subunit RICTOR drives breast cancer progression by promoting ganglioside biosynthesis through transcriptional and epigenetic mechanismsPLOS BiologySeptember 11, 2025Mohammad Nafees Ansari, Somesh K. Jha, Ali Khan, Kajal Rajput, Nishant Pandey, Dolly Jain, Rajeshwari Tripathi, Nihal Medatwal, Pankaj Sharma, Sudeshna Datta, et al.(Cat# P7626) Sigma, USA, 1× Protease inhibitor cocktail (Cat# R1329) Fermentas, USA, HindIII (Cat# R0104S) NEB, USA, Xhol (Cat# R0146S) NEB, USA, NEB Buffer 2.1 (Cat# B7202S) NEB, USA, N,N′-methylene bisacrylamide

(16) A novel linear B-cell epitope on S2 protein of transmissible gastroenteritis virus identified using a monoclonal antibodyBMC Veterinary ResearchSeptember 1, 2025Jinhui Hou, Ruifeng Hou, Hao Lu, Yang Yu, Rui zhao, Zhengting Shi, Yanrong Lv, Jinli Liu, Siyu Wang, Jin Yuan, et al.(New England Biolabs, Ipswich, MA, USA; Cat# R0101S) and HindIII (New England Biolabs, Ipswich, MA, USA; Cat# R0104S) restriction endonuclease sites. The amplified product was digested with the corresponding restriction

(17) MicroRNAs as regulators of NLRP3 inflammasome activation in herpes simplex virus type 2 infectionFrontiers in Cellular and Infection MicrobiologyMay 29, 2025Debashree Dass, Anwesha Banerjee, Ashwini More, Anupam MukherjeeUSA: AM5795) were digested with MluI and HindIII (New England Biolabs, Rowley, MA, USA: R0198S and R0104S) and ligated using T4 DNA Ligase (New England Biolabs, M0202S). Ligation products were transformed into

(18) The NONO protein regulates nonclassical DNA structure: Effects on circadian genes and DNA damageiScienceMay 16, 2025Ermanno Moriggi, Melissa Pisteljic, Alex Rosi-Andersen, Lennart Opitz, Abdelhalim Azzi, Steven A. Brown(USA),Cat# R0575S EcoRI,New England Biolabs (USA),Cat# R0101S HindIII,New England Biolabs (USA),Cat# R0104S SspI,New England Biolabs (USA),Cat# R0132S XbaI,New England Biolabs (USA),Cat# R0145S NEB CUT SMART

(19) MSH2 is not required for either maintenance of DNA methylation or repeat contraction at the FMR1 locus in fragile X syndrome or the FXN locus in Friedreich's ataxiaEpigenetics ChromatinApril 28, 2025Jessalyn Grant-Bier, Kathryn Ruppert, Bruce Hayward, Karen Usdin, Daman Kumarisamples, 2 µg of genomic DNA were digested for 3 h with HindIII restriction enzyme (New England Biolabs, R0104S) and stored overnight at -20 °C. Then, 200 ng of DNA was enzymatically bisulfite converted using the

(20) Intracellular Retention of Estradiol Is Mediated by GRAM Domain–Containing Protein ASTER-B in Breast Cancer CellsMolecular Cancer ResearchApril 1, 2025Hyung Bum Kim, W. Lee KrausE2621S) in pcDNA3 vectors that were linearized by digestion with XhoI (NEB, R0146S) and HindIII (NEB, R0104S). The G518F mutation was introduced into the pcDNA3-FLAG-GRAMD1B construct using the Q5 Site-Directed Mutagenesis Kit

Write a Review

(1)

9/28/2018Hind III Restriction Enzyme Jesús CirizaWe use this restriction enzyme for cloning. After cloning into a PCR product cloning vector, we digest with this enzyme to get the DNA fragment that will be next clone in the desired vector with the ... Read Review

HindIII

HindIII from New England Biolabs

Sequence

New England Biolabs

Citations (113)

Reviews

HindIII from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map