Agilent BioTek Synergy LX Multimode Reader from Agilent Technologies

Product Specs
ItemAgilent BioTek Synergy LX Multimode Reader
CompanyAgilent Technologies
Price Get Pricing Get Quote for this Product
Catalog NumberSynergy LX
QuantityEA
DetectionUV-Vis absorbance, Fluorescence intensity, Luminescence
Optical SystemMonochromator (UV/Vis) and filter-based (Fl, Lum)
Measuring TimeAbsorbance - 12 s (96 wells), 23 s (384 wells)
Fluorescence intensity - 24 s (96 wells), 76 s (384 wells)
Plate Formats6 to 384-well plates
Wavelength(s)200 to 999 nm (Abs), 320 to 700 nm (low noise PMT), 320 to 850 nm (red-shifted PMT)
Light SourceXenon flash / Halogen
DetectorPhotodiode or PMT
ApplicationsELISA, Fluorescence, Nucleic acid & protein quantification, Cell viability assays, Luminescence
SoftwareGen5™ Data Analysis Software; Optional Gen5 Secure for 21 CFR Part 11 compliance

Specifications/Features

Shaking

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Agilent Technologies

5301 Stevens Creek Blvd

Santa Clara, California 95051

United States

Phone: +1 877-424-4536

Company Profile

Website: www.agilent.com

(1) Full-length 16S rRNA nanopore sequencing enables species resolution of Fusobacterium associated with colorectal cancerGut MicrobesDecember 31, 2026William Rosenbaum, Marina Rubio Garcia, Anna Löfgren-Burström, Pär Larsson, Sofia Edin, Vicky Bronnec, Richard Palmqvist(Agilent) with Genomic DNA ScreenTape and reagents (Agilent). DNA concentrations were measured using a Synergy LX (Agilent) plate reader with the Qubit 1x dsDNA HS Assay Kit (Thermo Fisher Scientific). A total of 192

(2) Prevalence and activity of class II microcins in Serratia marcescens strains by isolation sourceApplied and Environmental MicrobiologyApril 22, 2026Jennifer K. Parker, Leon P. Toursarkissian, Joanna R. Chang, Simon Sanchez-Paiva, Angela C. O'Donnell, Halimot O. Badmus, Maria Patricia Nunez, Anne-Catrin Uhlemann, Bryan W. Davies(Greiner Bio-One); resulting luminescence (relative light units, RLU) was detected using the BioTek Synergy LX Multimode Reader (Agilent). One-way ANOVA with post hoc multiple comparisons was conducted to compare

(3) Loss of flavonol 3-O-glucosyltransferase activity confers soybean resistance to leaf-chewing insectsPlant JApril 15, 2026P. K. Prabhakar, M. A. Ortega, B.‐K. Ha, P. R. LaFayette, S. A. Harding, C. J. Tsai, B. R. Urbanowicz, H. R. Boerma, W. A. Parrottmanufacturer's instructions using 384‐well Corning 4513 white plates with luminescence measured using a Synergy LX Multi‐mode microplate reader (BioTek, USA). The released UDP was quantified using a standard curve. Matrix‐assisted

(4) Changes of urinary immunity and microbiome after intravesical BCG therapy and their association with outcomes in NMIBCExplor Target Antitumor TherApril 13, 2026Yuki Oda, Makito Miyake, Nobutaka Nishimura, Takuto Shimizu, Takuya Owari, Kota Iida, Yasushi Nakai, Nobumichi Tanaka, Kiyohide Fujimotourine sediment pellets and obtained as a purified DNA solution. DNA concentration was measured on a Synergy LX (Agilent Technologies, Agilent BioTek, Santa Clara, CA, USA) using the QuantiFluor dsDNA System (Promega Corporation

(5) Association between chronic stress and the epigenome: Exploration of psychological and biological stressPLoS OneApril 6, 2026Melissa Miller, Robin D. Brown, Cole Krautkramer, Rhea Gogia, Hagen Fritz, Dennis Wylie, Kerry Kinney, Frances A. Champagneenzyme-linked immunosorbent assay (ELISA) as specified by the manufacturer’s instructions (Enzo Life Sciences). The Biotek Synergy LX micro-plate reader (Agilent Technologies) was used to measure the optical density at 405 nm with a correction

(6) Mitochondrial presequences harbor variable strengths to maintain organellar functionJournal of Cell BiologyApril 6, 2026Youmian Yan, Baigalmaa Erdenepurev, Thiago N. Menezes, Ian Collinson, Natalie M. Niemiplate (Pro Lab Supply, catalog #30196) at room temperature. Luminescence was measured with a BioTek Synergy LX Multimode Reader (Agilent Technologies) using the luminescence filter and BioTek Gen5 software (Agilent

(7) Yeast Phenomic Analysis Reveals DNA Repair, pH Homeostasis, and Ribosomal Biogenesis as Modulators of Anticancer Ruthenium Complex KP1019Int J Mol SciApril 4, 2026Amanda F. Bible, Jackson S. Blackman, John W. Rodgers, Samuel R. Gary, Megan Rainey, Mary E. Miller, Alexander Stepanov, John L. Hartman 4th, Laura K. Stultz, Pamela K. Hansonphase and the prevention of condensation with pre-treatment of microtiter plate lids [121]. A BioTek Synergy LX plate reader controlled by Biotek Gen5 software version 3.10.06 (Agilent Technologies, Santa Clara,

(8) Anti-cryptosporidial efficacy of tricyclic carbazole aminoalcohols in vitro and in vivoInternational Journal for Parasitology: Drugs and Drug ResistanceApril 1, 2026Mingxiao Liu, Weisi Wang, Qi Zheng, Di Zhang, Dongqiang Wang, Chenchen Wang, Jigang Yin, Liping Duan, Guan Zhureagent was added, and plates were incubated for 2 h at 37 °C. Absorbance was measured at 490 nm using a Synergy LX multimode plate reader (BioTek). Cell viability was calculated relative to vehicle controls, and TC50

(9) Protocol for Nanodisc single-molecule pull-down assay to detect protein-lipid interactionsSTAR ProtocolsMarch 20, 2026Adriana Reyes-Ordoñez, Shweta Shree, Stephen G. Sligar, Jie Chenbottom, non-treated) (to read gfp concentrations on cell lysates)",Corning Inc.,Cat# BTLXSF1 Agilent Synergy LX plate reader,Agilent Technologies,Cat# SLXASN ÄKTA Pure Chromatography System,Cytiva,Model ÄKTA Pure

(10) Deep-learning-based de novo discovery and design of therapeutics that reverse disease-associated transcriptional phenotypesCellMarch 17, 2026Jing Xing, Mingdian Tan, Dmitry Leshchiner, Mengying Sun, Mohamed Abdelgied, Li Huang, Shreya Paithankar, Katie Uhl, Rama Shankar, Erika Lisabeth, et al.to solubilize formazan at 37°C for 1 h. Optical density (OD) values at 570 nm were measured using a SYNERGY-LX absorbance microplate reader (BioTek). The IC50 values were calculated through nonlinear curve fitting

(11) Ultrasonic-assisted extraction, purification, identification, and bioactivity evaluation of phlorotannins from Sargassum fusiforme: A comparative study of RSM and SVM-GAUltrason SonochemMarch 16, 2026Weibin He, Qimiao Kong, Haoran Li, Yuhan Wu, Runyan Huang, Li Yu, Shanshan Shen, Chaojian Xu, Yuehua Han, Yuting Ai, et al.the dark. The absorbance was measured at 510 nm by using a multifunctional microplate reader (BioTek Synergy LX, Agilent, USA). The result was determined based on the standard curve of phloroglucinol (mg PGE/g).

(12) Hydrogel Microdroplet Based Glioblastoma Drug Screening PlatformJournal of Biomedical Materials Research Part AMarch 14, 2026Brittany A. Payan, Annika Carrillo Diaz Diaz De Leon, Tejasvi Anand, Gunnar B. Thompson, Vishnu V. Krishnamurthy, Ana Mora‐Boza, Andrés J. García, Brendan A. C. HarleyQuant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific), and fluorescence was measured using a Synergy LX Multi-Mode Microplate Reader (BioTek, Winooski, VT) at 485 nm excitation wavelength and 528 nm emission

(13) Epithelial sensing of vitamin A shapes intestinal antimicrobial defensebioRxivMarch 13, 2026Gabriella Quinn, Daniel C. Propheter, Kartik Kulkarni, Matthew Johnson, Gonçalo Vale, Jeffrey G. McDonald, Ann Johnson, Brian Hassell, Cassie L. Behrendt, Nikhil V. Munshi, et al.Passive Lysis Buffer (Promega). Luminescence was measured using Bright-Glo reagents (Promega) on a BioTek Synergy LX multimode reader (Agilent). β-galactosidase activity from the pCMV-LacZ transfection control plasmid

(14) Pathological convergence of a bacterial plant pathogen is associated with the horizontal transfer of an effector-containing mobile elementBMC GenomicsMarch 13, 2026Elise M. Bull, Viplav Agarwal, Marcus M. Dillonconcentrations were estimated by measuring the absorbance at 600 nm with a multi-mode microplate reader (BioTek Synergy LX). Bacterial concentrations were then standardized to an OD600 of 0.0004 (approximate concentration of

(15) Short-Chain Fatty Acids and Palmitate Induce Distinct Metabolic and Phenotypic Signatures in Normal and Ischemic Skeletal Muscle Microvascular Endothelial CellsCellsMarch 10, 2026Andrew Guilfoyle-Speese, Kripa Patel, Aishwarya H. Ghanwat, David Stepp, Vijay Gantamanufacturer’s instructions. Post-incubation, the plates were colorimetrically read at 450 nM using a BioTek Synergy LX Multi-Mode Plate Reader (Agilent, Santa Clara, CA, USA. Each well in the 96-well plate was considered

(16) SIRT3 activation protects from nabumetone-induced mitochondrial toxicity in adult human cardiomyocytesCell Mol Life SciMarch 10, 2026Yafei Huang, Hong Liu, Chao Tong, Zhimin Wang, Miaomiao Xu, Mengqi Dong, Rongjia Rao, Xianqiang Wang, Wei Feng, Zhan Hu, et al.added to wash the cells. Then, red fluorescence intensity was detected by using a plate reader (BioTek, Synergy LX). A group of untreated cells was set up as a control in order to assess the effect of drugs or treatments

(17) Limosilactobacillus reuteri 3613-1 Delays Onset of Unconfirmed Urinary Tract Infections in Otherwise Healthy WomenMicroorganismsMarch 9, 2026Valentine Turpin, Charles Kakilla, Jessica Foote, Oliver Chen, William Hooper, Wafaa Ayad, Annahita Ghassemi, Noah Zimmermann, Kieran Rea, Amy Wescotttest day (24 or 48 h later), the culture in each well was gently resuspended and placed in a BioTek Synergy LX spectrophotometer (BioTek/Agilent, Winooski, VT, USA). OD600 was read, and the percentage inhibition

(18) An environmentally relevant phthalate mixture impairs ovulatory prostaglandin and progesterone receptor pathways in human granulosa cells in vitroEnvironment InternationalMarch 6, 2026Katie L. Land, Hong Xu, James W. Akin, Patrick R. Hannonsample was loaded per well. COX peroxidase activity was quantified colorimetrically at 590 nm using a Synergy LX multimode plate reader (Agilent BioTek), with isozyme-specific inhibitors included to distinguish COX2

(19) STIM1-Mitofusin2 interactions tether mitochondria and melanosome contacts that promote melanosome maturationNature CommunicationsMarch 6, 2026Isshin Shiiba, Yuto Ishikawa, Hijiri Oshio, Naoki Ito, Fuya Yamaguchi, Shun Nagashima, Hideya Ando, Keitaro Umezawa, Yuri Miura, Yuhei Araiso, et al.USA) was used according to the manufacturer’s instructions. Luminescence was then measured using a Synergy LX or HTX multimode reader (BioTek). Kinetic fluorescent probe measurements For kinetic fluorescent probe

(20) Biochemical characterization of xyloglucan galactosyltransferases MUR3 and XLT2 from Spirodela polyrhizaPlant JMarch 5, 2026Charles J. Corulli, Alexander S. Graf, Digantkumar Chapla, Liang Zhang, Samantha J. Ziegler, James Byrnes, Yannick J. Bomble, Kelley W. Moremen, Breeanna R. Urbanowicz, Pradeep K. Prabhakarmin at room temperature. Luminescence was recorded with a multifunctional microplate reader BioTek Synergy LX Multi‐Mode Reader (Agilent, Santa Clara, CA, USA). A standard curve was generated using UDP dilutions

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