DASware® control from Eppendorf

(1) Continuous collection of human mesenchymal-stromal-cell-derived extracellular vesicles from a stirred tank reactor operated under xenogeneic-free conditions for therapeutic applicationsStem Cell Res TherApril 24, 2025Cristiana Ulpiano, William Salvador, Teresa Franchi-Mendes, Min-Chang Huang, Yee-Hsien Lin, Han-Tse Lin, Carlos A. V. Rodrigues, Ana Fernandes-Platzgummer, Joaquim M. S. Cabral, Gabriel A. Monteiro, et al.oxygen. The glass vessel was treated with the siliconizing reagent Sigmacote® (Sigma-Aldrich) before use. DASware® control software (Eppendorf™) was employed to control the process parameters within the chosen set points (T

(2) Selective production of the itaconic acid-derived compounds 2-hydroxyparaconic and itatartaric acidMetabolic Engineering CommunicationsDecember 1, 2024Philipp Ernst, Felicia Zlati, Larissa Kever, Astrid Wirtz, Rainer Goldbaum, Jörg Pietruszka, Benedikt Wynands, Julia Frunzke, Nick Wierckxcultivations were carried out in a DASGIP® Bioblock (Eppendorf, Germany), controlled using the Eppendorf DASware® control software (Eppendorf, Germany). Vessels with a total volume of 2.3 L and a working volume of 1.0 L were

(3) Inhibition Control by Continuous Extractive Fermentation Enhances De Novo 2‐Phenylethanol Production by YeastBiotechnology and BioengineeringOctober 25, 2024Alessandro Brewster, Arjan Oudshoorn, Marion van Lotringen, Pieter Nelisse, Emily van den Berg, Marijke Luttik, Jean‐Marc Daranconcentration, and optical density. Off‐gas was analyzed using a DASGIP GA Offgas Analyzer and interpreted with DASware control software (Eppendorf). 2.4 Industrial Pilot‐Scale Continuous L‐L Extraction (FAST) Process 2.4.1 Seed

(4) Establishing an itaconic acid production process with Ustilago species on the low-cost substrate starchFEMS Yeast ResearchJuly 22, 2024Philipp Ernst, Astrid Wirtz, Benedikt Wynands, Nick Wierckx(Eppendorf, Germany) was used to conduct the batch fermentations, which were controlled using the Eppendorf DASware® control software (Eppendorf, Germany). Vessels with a total volume of 2.3 l and a working volume of 1.0 l were

(5) Balancing pH and yield: exploring itaconic acid production in Ustilago cynodontis from an economic perspectiveBiotechnology for Biofuels and BioproductsJuly 17, 2024Philipp Ernst, Katharina Maria Saur, Robert Kiefel, Paul-Joachim Niehoff, Ronja Weskott, Jochen Büchs, Andreas Jupke, Nick Wierckxperformed in a DASGIP® Bioblock (Eppendorf, Germany). The process was controlled using the Eppendorf DASware® control software (Eppendorf, Germany). Vessels with a total volume of 2.3 L and a working volume of 1.0 L were

(6) Expanding Pseudomonas taiwanensis VLB120's acyl‐CoA portfolio: Propionate production in mineral salt mediumMicrobial BiotechnologyJanuary 1, 2024Dário Neves, Daniel Meinen, Tobias B. Alter, Lars M. Blank, Birgitta E. Ebertwere performed in 2 L stirred‐tank bioreactors (Eppendorf, Germany) controlled by BioFlo120 units and DASware Control Software 5.3.1 (Eppendorf, Germany). A working volume of 1.5 L MSMB12 containing the required antibiotics

(7) High-quality physiology of Alcanivorax borkumensis SK2 producing glycolipids enables efficient stirred-tank bioreactor cultivationFrontiers in Bioengineering and BiotechnologyNovember 23, 2023Tobias Karmainski, Marie R. E. Dielentheis-Frenken, Marie K. Lipa, An N. T. Phan, Lars M. Blank, Till Tisoglass stirred-tank bioreactor with the BioFlo120 fermentation system (Eppendorf, Hamburg, Germany). The DASware control software controlled the bioprocess (v 5.3.1.; Eppendorf, Hamburg, Germany). For online data acquisition,

(8) Novel Insights into the Molecular Mechanisms Underlying Robustness and Stability in Probiotic BifidobacteriaApplied and Environmental MicrobiologyMarch 29, 2023Marie Schöpping, Anisha Goel, Kristian Jensen, Ricardo Almeida Faria, Carl Johan Franzén, Ahmad A. Zeidanin pH-controlled batch fermentations in a 250-mL DASGIP Parallel Bioreactor System, controlled using Dasware Control version 5.5.1 (Eppendorf). Cysteine-HCl and menaquinone-4 were added prior to inoculation at final concentrations

(9) Accelerated Adaptive Laboratory Evolution by Automated Repeated Batch Processes in Parallelized BioreactorsMicroorganismsJanuary 20, 2023Lukas Bromig, Dirk Weuster-Botzpump as part of this work. 2.4.3. Process Control Software The proprietary process control software (DASware® Control, Eppendorf AG, Hamburg, Germany) was used for the basic batch process control, such as pH control, DO

(10) Urea is a drop-in nitrogen source alternative to ammonium sulphate in Yarrowia lipolyticaiScienceDecember 22, 2022Oliver Konzock, Simone Zaghen, Jing Fu, Eduard J. Kerkhovenal.24,N/A JFYL007,Poorinmohammad et al.23,N/A ,, Software and algorithms,Software and algorithms,Software and algorithms ,, DASware® control 5 version 5.30,Eppendorf,https://www.eppendorf.com/gb-en/eShop-Products/Bioprocess/Bioprocess-Software/DASware-control-p-PF-133797 bcl2fastq_v2.20.0.422,Illumina,https://support.illumina.com/downloads/bcl2fastq-conversion-software-v2-20.html RNAseq

(11) Improved Itaconate Production with Ustilago cynodontis via Co-Metabolism of CO2-Derived FormateJournal of FungiDecember 5, 2022Lena Ullmann, Nils Guntermann, Philipp Kohl, Gereon Schröders, Andreas Müsgens, Giancarlo Franciò, Walter Leitner, Lars M. BlankBioFlo® 120 bioreactor with a total volume of 1.3 L and a working volume of 0.5 L in combination with DASware Control Software 5.3.1 (Eppendorf, Hamburg, Germany). Cultivations were performed in batch medium containing

(12) Optimization of the production process for the anticancer lead compound illudin M: process development in stirred tank bioreactorsMicrobial Cell FactoriesDecember 1, 2022Lillibeth Chaverra-Muñoz, Stephan Hüttelmeasurement, and infrared (IR) sensors (BlueSens, Germany) for CO2 measurement. Process was controlled by DASware® Control 5.6.2 (Eppendorf, Germany). Equipment dimensions are described in Table 5. Cultivation volume was 1500

(13) Online estimation of changing metabolic capacities in continuous Corynebacterium glutamicum cultivations growing on a complex sugar mixtureBiotechnology and BioengineeringFebruary 1, 2022Peter Sinner, Marlene Stiegler, Oliver Goldbeck, Gerd M. Seibold, Christoph Herwig, Julian Kagerparameters, gas and liquid inflow rates were retrieved online from the DASbox reactor system using the DASware control software (Eppendorf AG) (see Figure 1). CO2 and O2 off‐gas concentrations were measured online using

(14) Continuous human iPSC-macrophage mass production by suspension culture in stirred tank bioreactorsNature protocolsFebruary 1, 2022Ackermann M, Rafiei Hashtchin A, Manstein F, Carvalho Oliveira M, Kempf H, Zweigerdt R, Lachmann N.DASbox Mini bioreactor vessel for cell culture applications (Eppendorf, cat. no. 76DS0250ODSS) DASware® control (Eppendorf, cat. no. 78600167) Stereo microscope (Leica S9i, SZX16-ILLT, Olympus) Inverted fluorescence

(15) Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactorsSTAR protocolsDecember 17, 2021Manstein F, Ullmann K, Triebert W, Zweigerdt R.algorithms,Software and algorithms ,, FlowJo v10 software,BD Biosciences,N/A Fiji ImageJ,N/A,N/A DASware control,Eppendorf,Cat#78600167 Berkeley Madonna,University of California at Berkeley,N/A ,, Other,Other,Other ,, "10,

(16) Engineering Cupriavidus necator H16 for the autotrophic production of (R)-1,3-butanediolMetabolic EngineeringSeptember 1, 2021Joshua Luke Gascoyne, Rajesh Reddy Bommareddy, Stephan Heeb, Naglis Malys(405-DPAS-SC-K8S pH Probe, Mettler Toledo) and temperature Platinum RTD Temperature Sensor (Eppendorf). DASware® control software was used for automated control of DO, temperature, and pH. The preculture was prepared and

(17) High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cellsApplied Microbiology and BiotechnologyFebruary 1, 2021Yixiao Wu, Thomas Bissinger, Yvonne Genzel, Xuping Liu, Udo Reichl, Wen-Song Tanperfusion rate control, the output signal was transmitted through a ComBox (Hamilton, Switzerland) to the DASware® control software (Eppendorf, Germany). By converting the output signal to the perfusion rate, the software controlled

(18) Ustilaginaceae Biocatalyst for Co-Metabolism of CO2-Derived Substrates toward Carbon-Neutral Itaconate ProductionJournal of FungiJanuary 29, 2021Lena Ullmann, An N. T. Phan, Daniel K. P. Kaplan, Lars M. BlankBioFlo® 120 bioreactor with a total volume of 1.3 L and a working volume of 0.5 L in combination with DASware Control Software 5.3.1 (Eppendorf, Hamburg, Germany). Cultivations were performed in batch medium containing

(19) Uncoupling Foam Fractionation and Foam Adsorption for Enhanced Biosurfactant Synthesis and RecoveryMicroorganismsDecember 18, 2020Christian C. Blesken, Tessa Strümpfler, Till Tiso, Lars M. BlankProcedure for 2 L Scale The fermentation was performed using a BioFlo 120 bioreactor system with the DASware control (Version 5.0) software package (both from Eppendorf AG, Hamburg, Germany) in combination with two external

(20) Genetic Cell-Surface Modification for Optimized Foam FractionationFrontiers in Bioengineering and BiotechnologyOctober 29, 2020Christian C. Blesken, Isabel Bator, Christian Eberlein, Hermann J. Heipieper, Till Tiso, Lars M. Blankms. Fermentation Setup The fermentation was performed using a BioFlo 120 bioreactor system with a DASware control (Version 5.0) software package (both Eppendorf AG, Hamburg, Germany). The applied vessel with a total

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DASware® control

DASware® control from Eppendorf

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DASware® control from Eppendorf

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