RecJf from New England Biolabs

Product Specs
ItemRecJf
CompanyNew England Biolabs
Price $311.00 Inquire
Catalog NumberM0264L
Concentration30,000 units/ml
Quantity5000 units
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to produce 0.05 nmol TCA soluble deoxyribonucleotide in a total reaction volume of 50 µl in 30 minutes at 37°C in 1X NEBuffer 2 with 1.5 µg sonicated single-stranded [³H]-labeled E. coli DNA.

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infectionCell RepApril 7, 2026Manuel Garcia-Moreno, Azman Embarc-Buh, Robin Truman, Marko Noerenberg, Louisa Iselin, Honglin Chen, Caroline E. Lenz, Jeffrey Y. Lee, Kate Dicker, Snehith Dyavari Shetty, et al.U 5′ Deadenylase (New England Biolabs, #M0331S) and 15 U RecJf endonuclease (New England Biolabs, #M0264S) in 1X New England Biolabs buffer 2 with 20 U RNAsin, 20% PEG8000 for 1 h at 30°C and then 30 min at

(2) Integrative profiling of condensation-prone RNAs during early developmentCell GenomicsFebruary 11, 2026Tajda Klobučar, Jona Novljan, Ira A. Iosub, Boštjan Kokot, Iztok Urbančič, D. Marc Jones, Anob M. Chakrabarti, Nicholas M. Luscombe, Jernej Ule, Miha ModicAgencourt AMPure XP beads before adapter removal (using Deadenylase (M0331, NEB) and RecJf exonuclease (M0264S, NEB)). Reverse transcription was performed according to the Superscript IV RT kit (18090050, Invitrogen)

(3) Sequencing DNA methylation and hydroxymethylation at co-occurring chromatin featuresNature CommunicationsFebruary 10, 2026Rafael de Cesaris Araujo Tavares, Somdutta Dhir, Xuan He, Jack Monahan, Minna Taipale, Paula Golder, Aldo Ciau-Uitz, Walraj Gosal, David Tannahill, Shankar Balasubramanianexonuclease digestion mix (1X NEB buffer 4 NEB B7004S, 40 U T7 Exonuclease NEB M0263S, 120 U RecJf NEB M0264S, 80 U Exonuclease I from E. coli NEB M0293S) for 4 h at 37 °C, followed by an additional round of Zymo

(4) Genome-wide regulon of NtrC reveals genetic regulation under nitrogen limitation in Methylomonas sp. DH-1iScienceJanuary 16, 2026Sukjae Han, Joon Young Park, Yong Hee Han, Ina Bang, Hyeonwoo Mun, Ok Kyung Lee, Ji Sung Hyung, Jeong-Geol Na, Eun Yeol Lee, Donghyuk Kim, et al.,New England Biolabs,Cat#M0309S Lambda Exonuclease,New England Biolabs,Cat#M0262S RecJ Exonuclease,New England Biolabs,Cat#M0264S phi29 DNA Polymerase,New England Biolabs,Cat#M0269S T4 DNA Polymerase,New England Biolabs,Cat#M0203S KAPA HiFi HotStart ReadyMix

(5) CRISPR-free RNA base editing mediated PTC-readthrough restores hearing in mice with Otof nonsense mutationNature CommunicationsDecember 6, 2025Hanxiao Sun, Qi Teng, Wenqing Liu, Rui Guo, Menghua Li, Wei Xiong, Qiang Huang, Qianru Yu, Nan Luo, Yang Li, et al.at 25 °C for 2 h, followed by enzymatic digestion with 5’deadenylase (NEB, M0331S) and RecJf (NEB, M0264S) to remove excess adapter, and purification by ethanol precipitation. Reverse transcription was performed

(6) Pt-seq unveils the genomic binding pattern of platinum-based drugsSci AdvOctober 31, 2025Chuyun Shao, Mingyang Li, Liuyi Liu, Dongsheng Bai, Jinmin Yang, Cong Liu, Linxiang Cai, Xiaoyong Wang, Jinying Peng, Zijian Guo, et al.elution buffer at 56°C for 2 hours and then purified. Then, unligated DNA was removed by RecJf (NEB, M0264) and exonuclease I (NEB, M0293). Then, the primer extension was performed using Q5 High Fidelity DNA Polymerase

(7) Quantitative CRACI reveals transcriptome-wide distribution of RNA dihydrouridine at base resolutionNat CommunOctober 6, 2025Cheng-Wei Ju, Han Li, Bochen Jiang, Xuanhao Zhu, Liang Cui, Zhanghui Han, Junxi Zou, Yunzheng Liu, Shenghai Shen, Hardik Shah, et al.adding 2 µL 5′-deadenylase (NEB, M0331S) at 30 °C for 45 min, followed by 1 µL RecJf exonuclease (NEB, M0264L) at 37 °C for 45 min. RNA was again purified with RNA Clean & Concentrator and eluted in 10 µL RNase-free

(8) Critical impact of lysine 136 in TDP-43 phase separation, compartmentalization, and aggregation in living vertebratesiScienceJuly 18, 2025Cindy Maurel, Natalie M. Scherer, Luan Luu, Rowan A. Radford, Alison Hogan, Jessica Merjane, Andres Vidal-Itriago, Emily K. Don, Tyler Chapman, Stephen Cull, et al.deadenylated and removed using the following mix: 25U 5’ deadenylase (NEB M0331), 30U RecJf endonuclease (NEB M0264), 0.5U Rnasin (Promega, N2511), 10% BEN Buffer 2, 50% PEG8000. The mix was incubated for 1h at 30°C

(9) Small-molecule-catalysed deamination enables transcriptome-wide profiling of N6-methyladenosine in RNANature ChemistryJuly 1, 2025Pingluan Wang, Chang Ye, Michelle Zhao, Bochen Jiang, Chuan He5′-deadenylase (NEB, M0331S) and incubating at 30 °C for 30 min, followed by the addition of 1 μl RecJf (NEB, M0264L) for single-stranded DNA digestion at 37 °C for 30 min. The 3′-end-ligated RNA was purified using the

(10) Dual Ribosome Profiling reveals metabolic limitations of cancer and stromal cells in the tumor microenvironmentNature communicationsMay 19, 2025Aviles-Huerta D, Del Pizzo R, Kowar A, Baig AH, Palazzo G, Stepanova E, Amaya Ramirez CC, D'Agostino S, Ratto E, Pechincha C, et al.K227Q; NEB cat. M0351S), and excess linker removed with 5′ Deadenylase and RecJf (NEB cat. M0331S, cat. M0264S). Ribosomal RNA was depleted using biotinylated rRNA oligos (Supplementary Data 1) and streptavidin

(11) MapID-based quantitative mapping of chemical modifications and expression of human transfer RNACell Chemical BiologyMay 15, 2025Mitchel L. Tepe, Yitan Chen, Allison Carso, Huiqing Zhoual.31,N/A Nuclease P1,NEB,Cat# M0660S Proteinase K,NEB,Cat# P8107S Q5 Polymerase,NEB,Cat# M0491S RecJf,NEB,Cat# M0264S RNA Ligase 1,NEB,Cat# M0437M RT-1306,Zhou et al.31,N/A SSIII,Thermo Fisher,Cat# 18080093 T4 PNK,NEB,Cat#

(12) Translation dysregulation in cancer as a source for targetable antigensCancer cellMay 12, 2025Weller C, Bartok O, McGinnis CS, Palashati H, Chang TG, Malko D, Shmueli MD, Nagao A, Hayoun D, Murayama A, et al.Medium,Gibco,Cat#31985-047 Trypsin for cell culture,Corning,REF#25-051-CI 5’ Deanenylase,NEB,Cat#M0331S RecJf,NEB,Cat#M0264S 10mM dNTPs,Invitrogen,Cat#18427 Superscript III,Invitrogen,Cat#18080044 0.1M DTT,NEB,Cat#B1222A GlycoBlue™

(13) mTORC1 cooperates with tRNA wobble modification to sustain the protein synthesis machineryNat CommunMay 6, 2025Julia Hermann, Toman Borteçen, Robert Kalis, Alexander Kowar, Catarina Pechincha, Vivien Vogt, Martin Schneider, Dominic Helm, Jeroen Krijgsveld, Fabricio Loayza-Puch, et al.were treated with 5′ yeast deadenylase and RecJ exonuxlease (each 10 U/µl, New England Biolabs M0331S, M0264S) for 45 min at 30 °C to eliminate unbound adaptors. Next, rRNA homologous, biotinylated RNA oligos were

(14) True length of diverse capped RNA sequencing (TLDR-seq): 5'-3'-end sequencing of capped RNAs regardless of 3'-end statusNucleic Acids ResApril 4, 2025Jamie Auxillos, Arnaud Stigliani, Christian Skov Vaagensø, William Garland, Adnan Muhammed Niazi, Eivind Valen, Torben Heick Jensen, Albin Sandelindeadenylated and degraded using 25 units of 5′ deadenylase (NEB, #M0331S) and 15 units of RecJf (NEB, #M0264S) at 30°C for 30 min then purified with RNAClean XP Beads (Beckman Coulter, #A63987). A total of 2 μl

(15) Decoding m6Am by simultaneous transcription-start mapping and methylation quantificationElifeMarch 31, 2025Jianheng Fox Liu, Ben R Hawley, Luke S Nicholson, Samie R Jaffreyincubated in 50 μl adapter digestion reaction (40 μl water, 5 µl 10 X RNA ligase buffer, 1 µl RecJf [NEB #M0264S], 1 5’ Deadenylase [NEB #M0331S], 1 µl SUPERase·In) at 30 °C for 15 min then at 37 °C for 15 min. The

(16) Protocol for profiling RNA m5C methylation at base resolution using m5C-TAC-seqSTAR ProtocolsMarch 1, 2025Xiaoting Zhang, Liang Lu, Chengqi Yi, Xiaoyu Li"T4 RNA ligase 2, truncated",Beyotime,Cat#R0635L "T4 RNA ligase 1, high concentration",NEB,Cat#M0437M 5′ Deadenylase,NEB,Cat#M0331S RecJf,NEB,Cat#M0264S Proteinase K,NEB,Cat#P8102 Exonuclease I (E. coli),NEB,Cat#M0293S RLT buffer,QIAGEN,Cat#79216 ,, Critical

(17) The pseudorabies virus UL13 protein kinase triggers phosphorylation of the RNA demethylase FTO, which is associated with FTO-dependent suppression of interferon-stimulated gene expressionJournal of VirologyFebruary 25, 2025Ruth Verhamme, Robert J. J. Jansens, Jianheng Liu, Fien Van Raemdonck, Cliff Van Waesberghe, Luke Nicholson, Samie R. Jaffrey, Herman W. Favoreellibrary. The pooled RNA fragments were then treated by 5′ deadenylase (NEB #M0331S) and RecJf (NEB #M0264S), which can remove the exceeded 3′ adapter. After a second round of streptavidin enrichment, the RNA

(18) RACK1 MARylation regulates translation and stress granules in ovarian cancer cellsJ Cell BiolFebruary 3, 2025Sridevi Challa, Tulip Nandu, Hyung Bum Kim, Xuan Gong, Charles W. Renshaw, Wan-Chen Li, Xinrui Tan, Marwa W. Aljardali, Cristel V. Camacho, Jin Chen, et al.deadenylate the pre-adenylated linkers, which were then degraded by the 5′–3′ ssDNA exonuclease RecJ (M0264S; NEB). After rRNA reduction using the riboPOOL rRNA depletion kit (siTOOLs Biotech, Homo sapiens pool),

(19) Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptaseCommun BiolJanuary 11, 2025Zhiyong He, Weiqi Qiu, Huiqing Zhouadded into each ligation mixture by incubation at 30 °C for 1 h followed by adding 1 µL RecJf (NEB, M0264L) for ssDNA digestion at 37 °C for 1 h. Add 1 µL Proteinase K (Invitrogen, EO0491) 37 °C for 15 min.

(20) Ultrafast bisulfite sequencing detection of 5-methylcytosine in DNA and RNANature BiotechnologyOctober 1, 2024Qing Dai, Chang Ye, Iryna Irkliyenko, Yiding Wang, Hui-Lung Sun, Yun Gao, Yushuai Liu, Alana Beadell, José Perea, Ajay Goel, et al.adding 1 µl of 5′-deadenylase (NEB, M0331S) at 30 °C for 30 min followed by adding 1 µl of RecJf (NEB, M0264L) at 37 °C for 30 min. The 3′-end-ligated RNA was purified by RNA Clean and Concentrator (Zymo Research)

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RecJf

RecJf from New England Biolabs

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Specifications/Features

New England Biolabs

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RecJf from New England Biolabs

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