GloMax Discover System from Promega

Product Specs
ItemGloMax Discover System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberGM3000
QuantityEA
DetectionLuminescence, Fluorescence Intensity, UV-Visible Absorbance, Filtered Luminescence, BRET and FRET
Measuring Time96-well plate: less than 1 minute; 384-well plate: less than 3 minutes
Plate Formats6, 12, 24, 48, 96 and 384-well plates
ApplicationsAbsorbance, Luminescence, Fluorescence, ELISA, BRET, FRET
Optical System9 absorbance filters
Wavelength(s)200 to 600 nm
Sample Type6- to 384-well plates

Specifications/Features

Temperature control, Shaking, Injectors

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) TRPV4-driven ATP release activates CFTR through ADORA2B and P2RY2 receptors in the airway epitheliumiScienceSeptember 18, 2026Michele Genovese, Martina De Santis, Alessandro Giordano, Pina Marotta, Luis J.V. GaliettaLuminescence was recorded every 30 s for 22 measurements using a GloMax® Discover Microplate Reader (GM3000, Promega). During readings, one epithelium received 15 μL of Coon’s-HEPES containing Eact (5 μM) whereas

(2) Structural basis of diverse antibody recognition of conserved coronavirus spike S2 epitopes that contribute to protective immunitybioRxivSeptember 16, 2026Adonis A. Rubio, Virginia Crivelli, Václav Hönig, Monika Čížková, Tomás Cervantes Cervantes Rincón, Morgan E. Abernathy, Megan K. Parada, Danielle Vahdat, Yu E. Lee, Michael Eso, et al.and Nanoluc Luciferase activity of lysates measured using the Nano-Glo Luciferase Assay System with GloMax Discover System reader (Promega). Luminescence units were relative to those derived from cells infected with SARS-CoV-2

(3) AGC kinase homology requires and enables co-targeting for CNS regenerationbioRxivSeptember 7, 2026Hassan Al-Ali, Abdiel Badillo-Martinez, Bassel Awada, Brian B. Silver, Omar S. Elwardany, Ronald N. Buckle, Graham Johnson, Siyuan Sun, Rodrigo Lendof, Nikhita Guhan, et al.NanoLuc Inhibitor were added according to manufacturer instructions, and BRET signal was measured on a GloMax Discover System (Promega). A four-parameter logistic model was fitted to the normalized data in GraphPad Prism (v11.0)

(4) Novel TBX20 Variations Susceptible to Sporadic Atrial FibrillationBiomedicinesSeptember 3, 2026Zhen-Yu Xu, Dao-Liang Zhang, Xing-Biao Qiu, Chen-Xi Yang, Ying-Jia Xu, Yi-Qing Yang, Ning LiDual-Glo® Luciferase Assay System (Cat. No. E2940; Promega, Madison, WI, USA), performed using the GloMax® Discover System in accordance with the manufacturer’s instructions. The transcriptional activities of the KCNH2 and

(5) Behaviour of iPSC-Derived and Donor-Derived Hepatic Stellate Cells in Organoid Cultures Is Highly Variable and Contributes to Fibrotic OutcomeCellsAugust 28, 2026Yuwei Yang, Mina Kazemzadeh Dastjerd, Ayla Smout, Vincent Merens, Burak Toprakhisar, Catherine M. Verfaillie, Christophe Chesné, Leo A. van van Grunsvenaccording to the supplier’s instructions. The luminescence signal was measured by a GloMax (Promega, GM3000). Raw luminescence values were normalized to the mean luminescence of the corresponding control group

(6) An Effector TaCRVP From Tuta Absoluta Oral Secretion Impairs H2O2-Mediated Plant Defense by Targeting Tomato SlCAT2Adv Sci (Weinh)August 25, 2026Jianquan Yan, Youdan Zhang, Zhujun Wang, Huatao Tang, Huiping Liu, Zi Chen, Shuai Liu, Jiayi Shi, Lu Peng, Youming Hou(Sigma‐Aldrich, A8511, SML2236). Luminescence was continuously recorded every 1.5 min using a GloMax Discover GM3000 Multimode Microplate Reader (Promega, USA). ROS production dynamics were represented as relative light

(7) Targeting PKGIα Cys42 attenuates cardiac dysfunction in heart failure with preserved ejection fractionSci AdvAugust 21, 2026Jie Su, Yue Zhao, Pierre Coleman, Xiaoping Yang, Mark Holt, Janice Raabe, Friederike Cuello, Ajay Shah, Michael J. Shattock, Min Zhang, et al.incubation, luminescence was measured using a GloMax Discover Microplate Reader (Promega, catalog no. GM3000). In some experiments, 10 ng of recombinant PKGIα was treated with or without 10 mM DTT for 20 min,

(8) Systematic investigation reveals extensive Epstein-Barr virus transcriptional regulation of the human genomeCell GenomicsAugust 12, 2026Phillip J. Dexheimer, Matthew R. Hass, Lee E. Edsall, Arame A. Diouf, Sydney H. Jones, Omer Donmez, Cailing Yin, Katelyn A. Dunn, Carmy Forney, Hayley K. Hesse, et al.Technologies,Cat#G2992A iBLOT,Invitrogen,Cat#IB21001 Odyssey Dlx Imaging System,LI-COR Biosciences,Cat#2645119 GloMax Discover,Promega Corporation,Cat#GM3000 QuantStudio6 Flex Real-Time PCR System,Thermo Fisher Scientific,Cat#4485692 CHEMIDOC Imaging System,Bio-Rad,Cat#12003153

(9) Cardiac function in zebrafish embryos is linked to an androgen receptor-adrenomedullin-proepicardium axisCell Commun SignalJuly 30, 2026Max Duong Phu, Alessandra Guerrero Samanidis, Sabrina Laibacher, Martina Burczyk, Yara Alkhars, Ana Janovic, Ashraf Al Madhoun, Ilona S. Skerjanc, Martin D. Burkhalter, Melanie Philippand renilla luciferase activity were measured using the Dual Glo Luciferase Assay system and a GloMax GM3000 plate-reader (both Promega). Readings were background subtracted using results from untransfected cells

(10) Spatial modulation of RAF by RAF/MEK glue enables full-dose combination with pan-RAF inhibitor and potent RAS-mutant tumor-selective MAPK and growth inhibitionbioRxivJuly 30, 2026Ana Orive-Ramos, Bijaya Gaire, Christos Adamopoulos, Beau Baars, Mathieu Desaunay, Ziyue Kou, Evangelia Matenoglou, Silvia Coma, Nayeli Gutiérrez-Trejo, Kevin Mohammed, et al.recommendations, and the absorbance was read at 450 nM using the GloMax® Discover microplate reader (Promega, GM3000). Western blot and immunoprecipitation Immunoblotting and immunoprecipitation were performed according

(11) Protocol for Measuring Drug–Target Engagement in Mouse Colorectal Cancer Organoids Using NanoBRET AssayBio ProtocJuly 20, 2026Hammed A. Badmos, Colin Steele, Ross Cagancontroller (Falcon, catalog number: 357804) 16. Glomax Discover plate reader (Promega, catalog number: GM3000) Procedure A. Generation of organoid-compatible plasmid vector A1. Design and synthesis of lentiviral

(12) Development of an in vivo, screenable, split-luciferase based model of huntingtin multimerizationiScienceJuly 17, 2026Morgan G. Thomas, Simon A. Levy, Meredith H. Jenkins, Morgan Lambert, Bess Frost(Sigma-Aldrich #Z721417) and transferred to the plate reader (GloMax Discover Microplate Reader Promega #GM3000). After allowing flies to recover from anesthesia for 30 min, luminescence signal from each well was

(13) Analysis of West Nile disease convalescents identifies human monoclonal antibodies protective against West Nile and related orthoflavivirusesImmunityJuly 14, 2026Tomás Cervantes Rincón, Tereza Frckova, Zaria I. Contejean, Jasmine Cantergiani, Kevin Groen, Benedetta Cena, Simone G. Moro, Filippo Bianchini, Luca Simonelli, David Jarrossay, et al.μL lysis buffer added (Promega, E1531). The Nano-Glo Luciferase Assay System (Promega, N1120) with GloMAx Discover System reader (Promega) were used to measure the NanoLuc luciferase activity of the lysate. Serum was diluted

(14) Protocol for ex vivo mouse paired single-gonad screening platform for phenotypical characterizationSTAR ProtocJune 19, 2026Martín Andrés Estermann, Karina Flores Rodriguez, James Hunnicutt, Humphrey Hung-Chang Yaobottom",ThermoFisher Scientific,Cat#136101 HD Camera,Leica,DMC6200 GloMax Discoverer microplate reader,Promega,GM3000 Tissue-Tek Cryomold 10 x 10 x 5 mm,Sakura,Cat#4565 Superfrost Plus Microscope Slides,ThermoFisher

(15) Multiplexed cytokine and antigen mRNA administration generates durable anti-tumor immunity against pancreatic cancerNature CommunicationsJune 19, 2026Chaitanya N. Parikh, Kelly D. DeMarco, Nikita Bhalerao, Hadiya K. Giwa, Griffin I. Kane, Ronnie W. Dinnell, Boyang Ma, Haruka Mori, Meghan L. Brassil, Katherine C. Murphy, et al.methanol, and absorbance was measured at 560 nm (OD₅₆₀) using a microplate reader (Promega Glomax, GM3000). Background signal from blank wells was subtracted to obtain final OD values. To assess the impact

(16) Neuroprotective effects of C-phycocyanin and allophycocyanin from Spirulina purified using a newly developed method: in vitro barrier permeability, antioxidant and anti-inflammatory activitiesJournal of Biological EngineeringJune 18, 2026Antonella Girgenti, Pasquale Picone, Laura Palumbo, Rosaria Lauceri, Domenico Nuzzoadded to each well and incubated for 2 h at 37 °C, 5% CO₂. The absorbance was read at 490 nm using a GloMax® Discover System microplate reader (Promega). The results were expressed as a percentage of viability relative to the

(17) Identification of small molecules that enhance aminoglycoside-mediated suppression of CFTR and NF1 nonsense mutationsMolecular Therapy OncologyJune 18, 2026Joshua Sammons, Jianguo Chen, Kari Thrasher, Lianwu Fu, Ming Du, Hui Wen, J. Robert Bostwick, Paige Vinson, Omar Moukha-Chafiq, Corinne Augelli-Szafran, et al.perform the NanoLuc assay with the Nano-Glo luciferase assay system (Promega, Cat#N1120) using the GloMax Discover System (Promega). The lysate protein concentration was measured with the Bio-Rad Protein Assay Dye Reagent Concentrate

(18) Engineering Fc heterodimerization via asymmetric β-strand reconfiguration for bispecific antibody assemblyJournal of Biological EngineeringJune 18, 2026Arkaitz Cano, Izaskun Morillo, Ainhoa Goenaga, María Elena Laugieri, Ander de Blas, Eunate Valdaliso-Díez, June Ereño-Orbea, Beatriz Apellaniz, Edurne Rujas3 M HCl, and absorbance was measured at 450 nm using a GloMax® Discover Microplate Reader (Promega, GM3000). Differential scanning calorimetry Thermal stability of the engineered bsFc-D, along with the parental

(19) The transcription factor ZNF217 drives hepatocellular carcinoma progression by activating de novo lipid synthesis via FASN upregulationJournal of Cancer Research and Clinical OncologyJune 13, 2026Yihui Fang, Ting Zhang, Tianxing Ye, Qizhen He, Qinong Yesupernatants (20 μL) were transferred to opaque 96-well plates. Luciferase activities were quantified using a GloMax Discover System (Promega). Firefly luciferase activity was measured immediately after injection of 50 μL Luciferase Assay Reagent

(20) Signatures in the Protein Content of Human and Murine Blood Serum Exosomes, in the Context of Major Depressive Disorder, Are Associated with Cytokine ActivityCellsJune 6, 2026Jorge Manuel Vásquez-Pérez, Mónica Flores-Ramos, María Del Pilar Ramos-Godínez, Gerardo Bernabé Ramírez-Rodríguezinstructions. The 96-well plates were read in a Promega ELISA reader (Glomax Discover, Madison, WI, USA Ref#GM3000) [61,62,63]. 2.3.2. Evaluation of the Coat State (CS) and Weight of Mice Coat condition is described

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