LE220-plus Focused-ultrasonicator from Covaris LLC

Product Specs
ItemLE220-plus Focused-ultrasonicator
CompanyCovaris LLC
Price Get Pricing
Catalog Number500569
Dimensions(WxDxH) 24 x 30 x 19 inches (or 61 x 76 x 48 cm)
Quantity1
ApplicationsDNA/RNA/Chromatin Shearing:DNA/RNA/Total NA/Protein Extraction from FFPE:cfDNA Extraction from Plasma:DNA Extraction from Dried Blood Spots:Cell lysis: mammalian, bacterial, yeast:Tissue lysis and extraction
TypeFocused-ultrasonicator
Electrical Requirements100-240 V, ~50-60 Hz, 500 VAC maximum
Regulatory StatusCE and ETL Mark
TemperatureAmbient temperature: 19° C to 25° C (66° F to 77° C) Relative humidity: 30% to 70%
Capacity1 to 8 tubes at a time parallel processing; 96 tube capacity
Sample Volume5 to 500 µL
Volume5 to 500 µL
ComponentsIndividual microTUBEs:8 microTUBE strips (rack required):96 microTUBE plate:8 AFA-TUBE TPX Strip:96 AFA-TUBE TPX plate (with RFID):miniTUBEs:milliTUBEs

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Covaris LLC

14 Gill St, Unit H

Woburn, Massachusetts 01801-1721

United States

Phone: +1 781-932-3959
Fax: +1 781-932-8705

Email: info@covarisinc.com

Company Profile

Website: www.covaris.com

(1) The extinct Sicilian wolf shows a complex history of isolation and admixture with ancient dogsiScienceAugust 18, 2023Marta Maria Ciucani, Jazmín Ramos-Madrigal, Germán Hernández-Alonso, Alberto Carmagnini, Sabhrina Gita Aninta, Xin Sun, Camilla Hjorth Scharff-Olsen, Liam Thomas Lanigan, Ilaria Fracasso, Cecilie G. Clausen, et al.amplification and sequencing of modern Italian wolves and Cirneco dogs Extracts were fragmented in the Covaris LE220 plus Focused-ultrasonicator with the parameters set for getting 350-bp fragment length. The extracts were diluted to obtain 100

(2) Epigenetic dysregulation from chromosomal transit in micronucleiNatureJuly 6, 2023Albert S. Agustinus, Duaa Al-Rawi, Bhargavi Dameracharla, Ramya Raviram, Bailey S. C. L. Jones, Stephanie Stransky, Lorenzo Scipioni, Jens Luebeck, Melody Di Bona, Danguole Norkunaite, et al.and quality control by Agilent TapeStation, 97–165 ng genomic or micronuclear DNA was sheared using a LE220-plus focused ultrasonicator (Covaris) and sequencing libraries were prepared using the KAPA Hyper Prep Kit (Kapa Biosystems) with

(3) Sanitary installations and wastewater plumbing as reservoir for the long-term circulation and transmission of carbapenemase producing Citrobacter freundii clones in a hospital settingAntimicrobial Resistance & Infection ControlJune 19, 2023Hannelore Hamerlinck, Annelies Aerssens, Jerina Boelens, Andrea Dehaene, Michael McMahon, Anne-Sophie Messiaen, Stien Vandendriessche, Anja Velghe, Isabel Leroux-Roels, Bruno Verhasseltprepped for sequencing either by mechanical shearing to 200 base pairs (bp) fragments with Covaris LE220-plus Focused-Ultrasonicator (Covaris, Inc., Woburn, MA, USA) or via Illumina DNA PCR-Free Prep (Illumina Inc., San Diego, CA, USA).

(4) Profiling the human intestinal environment under physiological conditionsNatureMay 18, 2023Dari Shalon, Rebecca Neal Culver, Jessica A. Grembi, Jacob Folz, Peter V. Treit, Handuo Shi, Florian A. Rosenberger, Les Dethlefsen, Xiandong Meng, Eitan Yaffe, et al.Acoustics (AFA, Covaris) sonication in the 96-well plate for a total duration of 300 s per column with an LE220-plus Focused ultrasonicator (Covaris) using the following parameters: peak power, 450 W; duty factor, 50%; cycles, 200; average

(5) Subclonal somatic copy number alterations emerge and dominate in recurrent osteosarcomabioRxivJanuary 27, 2023Michael D. Kinnaman, Simone Zaccaria, Alvin Makohon-Moore, Brian Arnold, Max Levine, Gunes Gundem, Juan E. Arango Arango Ossa, Dominik Glodzik, M. Irene Rodríguez-Sánchez, Nancy Bouvier, et al.131–500ng of genomic DNA was sheared using an LE220-plus Focused-ultrasonicator (Covaris catalog # 500569), and sequencing libraries were prepared using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with

(6) Whole-exome sequencing identified recurrent and novel variants in benzene-induced leukemiaBMC Medical GenomicsJanuary 26, 2023Dafeng Lin, Dianpeng Wang, Peimao Li, Lihua Deng, Zhimin Zhang, Yanfang Zhang, Ming Zhang, Naixing ZhangMolecular Devices, USA), and about 200 ng of each DNA sample were randomly fragmented by Covaris sonication (LE220-plus Focused-ultrasonicator, Covaris, USA). The DNA fragments with the size mainly distributed between 150 and 250 bp were repaired

(7) Ovarian cancer mutational processes drive site-specific immune evasionNatureDecember 22, 2022Ignacio Vázquez-García, Florian Uhlitz, Nicholas Ceglia, Jamie L. P. Lim, Michelle Wu, Neeman Mohibullah, Juliana Niyazov, Arvin Eric B. Ruiz, Kevin M. Boehm, Viktoria Bojilova, et al.BioAnalyzer, 500 ng of genomic DNA was sheared using an LE220-plus focused ultrasonicator (Covaris, 500569) and sequencing libraries were prepared using the KAPA Hyper Prep kit (Kapa Biosystems, KK8504) with

(8) Pre-analytical processing of plasma and serum samples for combined proteome and metabolome analysisFrontiers in Molecular BiosciencesDecember 20, 2022Hagen M. Gegner, Thomas Naake, Aurélien Dugourd, Torsten Müller, Felix Czernilofsky, Georg Kliewer, Evelyn Jäger, Barbara Helm, Nina Kunze-Rohrbach, Ursula Klingmüller, et al.processed in a 1 μg/μl concentration and 100 mM ammonium bicarbonate (ABC, Sigma-Aldrich) using a Covaris LE220-plus focused-ultrasonicator for AFA-based ultrasonication in a 96-well format. The plate was transferred to a Bravo pipetting system

(9) Identification of a missense variant in the porcine AGPAT gene family associated with intramuscular fat content through whole‐genome sequencingAnimal GeneticsDecember 1, 2022Eduard Molinero, Ramona N. Pena, Joan Estany, Roger Ros‐FreixedesKAPA HyperPrep kit (Roche) with some modifications. In short, 0.4–1.0 μg of genomic DNA was sheared on a Covaris™ LE220‐Plus focused‐ultrasonicator (Covaris) in order to reach the fragment size of ~400 bp. The fragmented DNA was size‐selected for the

(10) Feasibility of whole genome and transcriptome profiling in pediatric and young adult cancersNature CommunicationsDecember 1, 2022N. Shukla et al.BioAnalyzer, 500 ng of genomic DNA were sheared using a LE220-plus Focused-ultrasonicator (Covaris catalog # 500569) and sequencing libraries were prepared using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with

(11) Clonal somatic copy number altered driver events inform drug sensitivity in high-grade serous ovarian cancerNature CommunicationsOctober 26, 2022Filipe Correia Martins, Dominique-Laurent Couturier, Ines de Santiago, Carolin Margarethe Sauer, Maria Vias, Mihaela Angelova, Deborah Sanders, Anna Piskorz, James Hall, Karen Hosking, et al.were diluted to 75 ng in 15 µl of PCR certified water for subsequent shearing by sonication using the LE220-plus Focused-Ultrasonicator (Covaris) for 120 s at RT (duty factor 30%; Peak incident power 180 W; 50 cycles per burst) with a target

(12) Visual inspection reveals a novel pathogenic mutation in PKD1 missed by the variant caller in whole‑exome sequencingMolecular Medicine ReportsOctober 24, 2022Bee Tee Koay, Mei Yee Chiow, Jamiila Ismail, Norfarhana Khairul Fahmy, Seow Yeing Yee, Norhazlin Mustafa, Masita Arip, Adiratna Mat Ripen, Saharuddin Bin MohamadQIAxpert System (Qiagen, GmbH). Two µg of genomic DNA was fragmented into 150–200 bp using Covaris LE220-plus focused-ultrasonicator (Covaris, Inc.). A genomic library with fragment size of ~330 bp was constructed using SureSelectXT

(13) PRC2-Inactivating Mutations in Cancer Enhance Cytotoxic Response to DNMT1-Targeted Therapy via Enhanced Viral MimicryCancer DiscoverySeptember 2, 2022Amish J. Patel, Sarah Warda, Jesper L.V. Maag, Rohan Misra, Miguel A. Miranda-Román, Mohini R. Pachai, Cindy J. Lee, Dan Li, Naitao Wang, Gabriella Bayshtok, et al.BioAnalyzer, 500 ng of genomic DNA was sheared using an LE220-plus Focused-ultrasonicator (Covaris, cat. #500569). Sequencing libraries were prepared using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) without

(14) Dynamic expression of SNAI2 in prostate cancer predicts tumor progression and drug sensitivityMolecular OncologyJuly 1, 2022Ying Z. Mazzu, YuRou Liao, Subhiksha Nandakumar, Martin Sjöström, Lina E. Jehane, Romina Ghale, Barani Govindarajan, Travis A. Gerke, Gwo‐Shu Mary Lee, Jian‐Hua Luo, et al.sequencing. Five hundred nanogram genomic DNA from four PC cells were used for the library preparation. An LE220‐plus Focused‐ultrasonicator (Covaris, Inc., Woburn, MA, USA) was used to shear 500 ng of genomic DNA. Sequencing libraries were

(15) Cleavage-Polyadenylation Factor Cft1 and SPX Domain Proteins Are Agents of Inositol Pyrophosphate Toxicosis in Fission YeastmBioFebruary 22, 2022Beate Schwer, Angad Garg, Ana M. Sanchez, Mindy A. Bernstein, Bradley Benjamin, Stewart Shumanthe four SST strains were sheared using a LE220-plus focused ultrasonicator (Covaris catalog number 500569), and sequencing libraries were prepared using the KAPA Hyper Prep kit (Kapa Biosystems KK8504) with

(16) Genetic screen for suppression of transcriptional interference reveals fission yeast 14–3–3 protein Rad24 as an antagonist of precocious Pol2 transcription terminationNucleic Acids ResearchJanuary 25, 2022Angad Garg, Stewart Shuman, Beate Schwerng aliquots of genomic DNA were sheared using a LE220-plus Focused-ultrasonicator (Covaris catalog #500569) and sequencing libraries were prepared using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with

(17) A scalable high-throughput targeted next-generation sequencing assay for comprehensive genomic profiling of solid tumorsPLOS ONEDecember 2, 2021Jeffrey M. Conroy, Sarabjot Pabla, Sean T. Glenn, R. J. Seager, Erik Van Roey, Shuang Gao, Blake Burgher, Jonathan Andreas, Vincent Giamo, Melissa Mallon, et al.Genomic DNA and total RNA were simultaneously extracted using the truXTRAC FFPE extraction kit and LE220-plus Focused-ultrasonicator (Covaris, Inc., Woburn, MA), as previously described [47], integrated with the Lynx robotic liquid handler

(18) Multi-omic analysis of lung tumors defines pathways activated in neuroendocrine transformationCancer DiscoveryDecember 1, 2021Alvaro Quintanal-Villalonga et al.BioAnalyzer, 170–750 ng of genomic DNA were sheared using a LE220-plus Focused-ultrasonicator (Covaris, #500569). Samples were cleaned using Sample Purification Beads from the TruSeq Methyl Capture EPIC LT Library Prep

(19) Whole-genome sequencing reveals progressive versus stable myeloma precursor conditions as two distinct entitiesNature CommunicationsDecember 1, 2021Bénedith Oben et al.Bio-Analyzer, 500 ng of gDNA were sheared using a LE220-plus Focused-ultrasonicator (Covaris catalog # 500569) and sequencing libraries were prepared using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with

(20) Comprehensive molecular characterization of lung tumors implicates AKT and MYC signaling in adenocarcinoma to squamous cell transdifferentiationJournal of hematology & oncologyOctober 16, 2021Quintanal-Villalonga A, Taniguchi H, Zhan YA, Hasan MM, Chavan SS, Meng F, Uddin F, Allaj V, Manoj P, Shah NS, et al.BioAnalyzer, 170–750 ng of genomic DNA was sheared using a LE220-plus Focused-ultrasonicator (Covaris, #500569). Samples were cleaned using Sample Purification Beads from the TruSeq Methyl Capture EPIC LT Library Prep

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