EcoRV-HF from New England Biolabs

Product Specs
ItemEcoRV-HF
CompanyNew England Biolabs
Price $67.00 Inquire
Catalog NumberR3195S
Quantity4000 units
Concentration20,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GAT⇓ATC
CTA⇑TAG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Genetic interaction between Adgrg6 and Sox9 reveals a feedforward mechanism for postnatal spinal stabilitybioRxivApril 8, 2026Valeria Aceves, Ziyi Xu, Cheng-Hai Zhang, Jongkil Kim, Joshua Ito, Kylie Mathers, Andrew B. Lassar, Zhaoyang Liu, Ryan S. GrayScience,470302-528 Acetone,Fisher Chemical,A38-212 Agarose,VWR,N605 DNase I,NEB,M0303A EcoRV-HF,NEB,R3195S Ethanol,"Decon Labs, Inc.",2716 Glycerol,Sigma Aldrich,G7893-500ML GoTaq Green,Promega,M7123 Instant

(2) Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systemsbioRxivMarch 30, 2026Philipp Koch, Frederic S. W. Arendrup, Chaehee Lim, Samyukta Narayanan, Amina Adam, Massimiliano Clamer, Anders H. Lund, Chun-Kan Chen, Kathrin LeppekmRuby-circEGFP-ScaI-v3 plasmid (pKL480), kindly provided by Chun-Kan Chen, was digested using the EcoRV-HF (NEB, R3195S) and ScaI-HF (NEB, R3122S) restriction enzymes according to manufactures instructions were used for

(3) Protocol for targeted gene manipulation and thermogenic evaluation in mouse brown adipocytesSTAR ProtocolsMarch 1, 2026Jakub Bunk, Drishti Soni, Matthias Calderon, Bozena Samborska, Lawrence Kazak1×, pH 7.4, without calcium and magnesium",Wisent Inc.,311-010-CL EcoRV-HF,New England Biolabs (NEB),R3195S "DNase I, RNase-free (1 U/μL) Kit",Thermo Scientific,EN0521 DNA/RNA Extraction Reagent - ViralXpress,Sigma-Aldrich,3095 2-Propanol

(4) A genome-scale single-cell CRISPRi map of trans gene regulation across human pluripotent stem cell linesCell GenomicsFebruary 11, 2026Claudia Feng, Elin Madli Peets, Yan Zhou, Luca Crepaldi, Sunay Usluer, Alistair Dunham, Jana M. Braunger, Jing Su, Magdalena E. Strauss, Daniele Muraro, et al.proteins","Chemicals, peptides, and recombinant proteins" ,, BbsI,NEB,R0539L NotI,NEB,R0189S EcoRV-HF,NEB,R3195S ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Gibson Assembly® Master Mix

(5) The RNA-binding protein PRRC2B preserves 5' TOP mRNA during starvation to maintain ribosome biogenesis during nutrient recoveryNucleic Acids ResDecember 11, 2025Nadav Goldberg, Doron Bril, Miriam Eisenstein, Tsviya Olender, Alon Savidor, Shani Bialik, Shmuel Pietrokovski, Adi KimchiSuperScript III (Invitrogen, cat# 18 080 044); Restriction enzymes KpnI, EcorV, DpnI (NEB, cat# R3142S, R3195S, R0176S). Instruments used included: for fluorescence microscopy, a LSM900 confocal microscope (Zeiss);

(6) Ribozyme-Mediated Knockdown of lncRNA Gene Expression in DrosophilaBIO-PROTOCOLOctober 20, 2025Kevin G. Nyberg, Richard W. Carthewnumber: M0302L) 8. EcoRV-HF (4,000 units at 20,000 units/mL) (New England Biolabs, catalog number: R3195S) 8. NEBuilder HiFi DNA Assembly Master Mix (50 reactions) (New England Biolabs, catalog number: E2621L) 9.

(7) Architectural transcription factors collectively shape nuclear radial positioning of chromatin contactsSci AdvAugust 29, 2025Juan Bai, Hanhan Wei, Siling Hu, Yi Guo, Qifan Zhang, Xiaokun Liu, Chenhuan XupCRIS-PITChv2-dTAG-BSD (BRD4) (Addgene, 91795) plasmid was digested with the restriction enzymes Eco RV–HF (NEB, R3195S) and Xba I (NEB, R0145V), and the large fragment was purified with agarose gel electrophoresis. The

(8) Unraveling mitochondrial influence on mammalian pluripotency via enforced mitophagyCellAugust 21, 2025Daniel A. Schmitz, Seiya Oura, Leijie Li, Yi Ding, Rashmi Dahiya, Emily Ballard, Carlos Pinzon-Arteaga, Masahiro Sakurai, Daiji Okamura, Leqian Yu, et al.HiFi PCR Premix,Takara,639298 HindIII-HF,New England Biolabs,R3104S EcoRV-HF,New England Biolabs,R3195S Critical commercial assays,Critical commercial assays,Critical commercial assays "Click-iT™ EdU Cell

(9) A chimeric Mla-Pqi lipid transport system is required for Brucella abortus survival in macrophagesThe EMBO JournalAugust 13, 2025Adélie Lannoy, Alexi Ronneau, Miguel Fernández-García, Marc Dieu, Patricia Renard, Antonia García Fernández, Raquel Condez-Alvarez, Xavier De Bolle,New England Biolabs,A9518 GoTaq® DNA polymerase,Promega,M7848 T4 DNA ligase,Invitrogen,100004917 EcoRV,New England Biolabs,R3195S Sodium deoxycholate,VWR,0613-50 g Proteinase K,Merck,740506 "Dulbecco’s Modified Eagle Medium (DMEM),

(10) Rainbow Nucleus Charts Dynamic Interactome of Membrane-less OrganellesBIORXIVMay 15, 2025Songtao Ye, Nadav Benhamou Goldfajn, Choon Leng So, Takanari Inoue, Danfeng Caidownstream sequences for cloning into the pUC57-Mini vector. pUC57-Mini was linearized by EcoRV-HF® (R3195S, NEB) and purified (QIAquick PCR Purification Kit, 28104, QIAGEN). The synthetic gene fragments and

(11) Cell expansion for notochord mechanics and endochondral bone lengthening in zebrafish depends on the 5′-inositol phosphatase Inppl1aCurrent BiologyMay 5, 2025Brittney Voigt, Katherine Frazier, Donya Yazdi, Jace Klein, Paul Gontarz, Bo Zhang, Diane S. Sepich, Julia Mo, Joanna Smeeton, Lilianna Solnica-Krezel, et al.BODIPY™ TR Methyl Ester,Invitrogen,C34556 Cytoseal 60,EMS,18006 DNAse I,NEB,M0303A EcoRV-HF,NEB,R3195S Ethanol,"Decon Labs, Inc.",2716 Fast-Green,Fisher,BP123-10 Glycerol,Sigma Aldrich,HHS15 GoTaq Green,Promega,M7123 HCR

(12) Short-term alterations in dietary amino acids override host genetic susceptibility and reveal mechanisms of Salmonella Typhimurium small intestine colonizationbioRxivMarch 29, 2025Nicolas G. Shealy, Madi Baltagulov, Camila de Brito, Anna McGovern, Pollyana Castro, Alexandra C. Schrimpe-Rutledge, Clara Malekshahi, Simona G. Condreanu, Stacy D. Sherrod, Somnath Jana, et al.Mix,BioRad,Cat#:1708880 Platinum™ Super FI II Master Mix,ThermoFis cher,Cat#:12368010 EcoRV-HF,NEB,Cat#:R3195S NEBuilder® HiFi DNA Assembly Master Mix,NEB,Cat#:E2621L Monarch® Spin PCR & DNA Cleanup Kit (5 μg),NEB,Cat#:T1130L Platinum PCR Supermix

(13) Single component CRISPR-mediated base-editors for Agrobacterium and their use to develop an improved suite of strainsBioDesign ResearchMarch 1, 2025Vincent J. Pennetti, Peter R. LaFayette, Wayne Allen ParrottpVPHDB-Ke/pVP074 using 1 μg of plasmid, 0.5 μL of each HindIII-HF (NEB #R3104S) and EcoRV-HF (NEB #R3195S), and 1 μL of CutSmart Buffer (NEB #B6004S) and incubated for 1 h at 37 °C prior to a 20-min heat

(14) Microvilli control the morphogenesis of the tectorial membrane extracellular matrixDevelopmental CellMarch 1, 2025Ava Niazi, Ju Ang Kim, Dong-Kyu Kim, Di Lu, Igal Sterin, Joosang Park, Sungjin Park,New England Biolabs,Cat# R0157S XhoI,New England Biolabs,Cat# R0146S EcoRV-HF,New England Biolabs,Cat# R3195S AscI,New England Biolabs,Cat# R0558S HindIII-HF,New England Biolabs,Cat# R3104S FseI,New England Biolabs

(15) Coordination between ESCRT function and Rab conversion during endosome maturationThe EMBO JournalFebruary 5, 2025Daniel P Ott, Samit Desai, Jachen A Solinger, Andres Kaech, Anne Spangstabilized),Sigma-Aldrich,Cat #P4333-20ML Fluoromount-G,SouthernBiotech,Cat #0100-01 EcoRV-HF,New England Biolabs,Cat #R3195S Software,Software,Software Fiji/ImageJ2,"https://imagej.net/software/fiji; Rueden et al, 2017",N/A MultiStackReg,"https://biii.eu/multistackreg;

(16) Atlas of multilineage stem cell differentiation reveals TMEM88 as a developmental regulator of blood pressureNat CommunFebruary 4, 2025Sophie Shen, Tessa Werner, Samuel W. Lukowski, Stacey Andersen, Yuliangzi Sun, Woo Jun Shim, Dalia Mizikovsky, Sakurako Kobayashi, Jennifer Outhwaite, Han Sheng Chiu, et al.plasmids AAVS1-CAG-hrGFP (Addgene #52344) was digested by incubation with EcoRV-HF (New England BioLabs; NEB #R3195S) followed by addition of MluI-HF (NEB #R3198S) and further incubation. Successful digestion was confirmed

(17) MCTS2 and distinct eIF2D roles in uORF-dependent translation regulation revealed by in vitro re-initiation assaysThe EMBO journalFebruary 1, 2025Meurs R, De Matos M, Bothe A, Guex N, Weber T, Teleman AA, Ban N, Gatfield D.,New England Biolabs,Cat. No. R0136S XhoI,New England Biolabs,Cat. No. R0146S EcoRV,New England Biolabs,Cat. No. R3195S BsaI-HF v2,New England Biolabs,Cat. No. R3733S AatII,New England Biolabs,Cat. No. R0117S SacI-HF,New England Biolabs

(18) Footprint-C reveals transcription factor modes in local clusters and long-range chromatin interactionsNature CommunicationsDecember 30, 2024Xiaokun Liu, Hanhan Wei, Qifan Zhang, Na Zhang, Qingqing Wu, Chenhuan XupCRIS-PITChv2-dTAG-BSD (BRD4) (AddGene, 91795) plasmid was digested with the restriction enzymes EcoRV-HF (NEB, R3195S) and XbaI (NEB, R0145V), and the large fragment was purified with agarose gel electrophoresis. The four

(19) A genetic and microscopy toolkit for manipulating and monitoring regeneration in Macrostomum lignanoCell ReportsNovember 26, 2024R. Nelson Hall, Hongquan Li, Chew Chai, Sidney Vermeulen, Robin R. Bigasin, Eun Sun Song, Souradeep R. Sarkar, Jesse Gibson, Manu Prakash, Andrew Z. Fire, et al.proteins" ,, Artificial Sea Water salts,Bulk Reef Supply,Cat# 208269 F/2 Media,Bigelow,Cat# MKf250L EcoRV-HF,NEB,Cat# R3195S 10× T4 DNA Ligase Buffer,NEB,Cat# B0202S T4 DNA Ligase,NEB,Cat# M0202L BsaI-HFv2,NEB,Cat# R3733S 23

(20) Protocol for evaluating the E3 ligase activity of BRCA1-BARD1 and its variants by nucleosomal histone ubiquitylationSTAR ProtocolsSeptember 20, 2024O’Taveon Fitzgerald, Bo Wu, Meiling Wang, Rouf Maqbool, Wenjing Li, Weixing Zhaogel,Sigma-Aldrich,Cat#A2220-1ML Clarity Max Western ECL substrate,Bio-Rad,Cat#1705062 EcoRV-HF,NEB,Cat#R3195S GeneJET Plasmid Miniprep Kit,Thermo Fisher Scientific,Cat#K0502 ClonExpress II One Step Cloning Kit,Cellagen

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10/7/2015High Fidelity Restriction Enzyme for Cloning Prabhakaran KumarI used this EcoRV restriction enzyme along with BglII for the cloning of my gene of interest into a plasmid vector. Subsequently I used this enzyme for colony screening also. This enzyme completely ... Read Review

EcoRV-HF

EcoRV-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (52)

Reviews

EcoRV-HF from New England Biolabs

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