EcoRI from New England Biolabs

Product Specs
ItemEcoRI
CompanyNew England Biolabs
Price $66.00 Inquire
Catalog NumberR0101S
Concentration20-100 u/µL
Quantity10000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

G/AATTC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Genome-wide mapping reveals increased R-loops in topoisomerase III-beta-deficient human cellsiScienceSeptember 18, 2026Sourav Saha, Anjali Dhall, Shar-Yin Naomi Huang, Liton Kumar Saha, Md Rasel Al Mahmud, Hongliang Zhang, Yilun Sun, Yves Pommier25530049 HindIII,New England Biolabs,Cat# R0104S SspI,New England Biolabs,Cat# R3132 EcoRI,New England Biolabs,Cat# R0101S BsrGI,New England Biolabs,Cat# R3575S XbaI,New England Biolabs,Cat# R0145S "Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v

(2) Preclinical evaluation of AAV2 anti-VEGF gene therapy using scFv for choroidal neovascularisationMolecular Therapy AdvancesSeptember 10, 2026Xin Xu, Guangzuo Luopreviously described with modification.41 Briefly, human genomic DNA was digested with EcoRI (NEB, R0101S), followed by two phenol-chloroform-isoamyl alcohol (25:24:1) extractions, ethanol precipitation, and

(3) Compact Cas12f enables genome editing in avian cellsPoultry ScienceSeptember 1, 2026Yujin Han, Seung Je Woo, Hee Jung Choi, Jae Yong Hancassette with a puromycin resistance gene through digestion and ligation using EcoRI restriction enzyme (#R0101L, New England Biolabs, MA, USA) and T4 ligase (#2011A, Takara, Shiga, Japan). gRNA sequences (Table 1)

(4) The dual specificity phosphatase 2 act as distal regulatory node in T cell signalingiScienceAugust 21, 2026Henrike Bruckmueller, Anangi Balasiddaiah, Sofia Malek, Victoria Tenhaken, Julien Bruckmueller, Jakob Mejlvang, Helene Spangenberg, Farah Syed, Bjarne Johansen, Hanne Kildalsen, et al.,Sigma Aldrich,P8833 SCH772984,"MedChemExpress,",HY-50846 BamH1-HF,New England Biolabs,R3136S EcoRI,New England Biolabs,R0101S Kpn1-HF,New England Biolabs,R3142S XhoI,New England Biolabs,R0146S XbaI,New England Biolabs,R0145S GST-ERK1,MRCPPU

(5) hnRNPC facilitates coronavirus replication by directly binding the frameshift-stimulatory element of viral genomic RNAPLoS PathogAugust 7, 2026Jingchen Xu, Hongying Li, Jianrui Li, Biao Dong, Hu Li, Han Sun, Jiayu Zhang, Jiujiao Gao, Yue Gong, Xinyue Liu, et al.reticulocyte lysate The plasmid (pcDNA3.1(+)-mCherry-FSECoV2-EGFP) was digested with EcoR I (NEB, R0101S) at 37°C for 3 hours and then treated with Mung Bean Nuclease (NEB, M0250S) at 30°C for 30 minutes.

(6) Ketamine persists within neuronal compartments long after systemic clearanceResearchSquareJuly 22, 2026Joseph Cichon, Kallol Bera, Zachary Blumenfeld, Elaine Lin, Meah Ahmed, Eve Fine, Md. Nadim Hossain, Andrey Andreev, Altyn Rymbek, Peter Fenton, et al.competent Dpn1,NEB,R0176S,Restriction enzyme BamHI,NEB,R0136S,Restriction enzyme PstI,NEB,R0140S,Restriction enzyme EcoRI,NEB,R0101S,Restriction enzyme HindIII,NEB,R0104S,Restriction enzyme SalI,NEB,R0138S,Restriction enzyme NotI,NEB,R0189S,Restriction

(7) Neuron-derived neurotrophic factor-positive interneurons: a cellular target for anti-seizure therapiesEBioMedicineJuly 21, 2026Amy Richardson, Marion S. Mercier, Yoshiteru Shimoda, Robert T. Graham, Marco Leite, Alejandro Garcia, Maud Muller, Suraya A. Bond, Maria Grozdanova, Qimin Wu, et al.fragment and the pAAV-EF1α-FLEX-Chronos-GFP were digested using PacI (NEB; R0547S) and EcoRI (NEB; R0101S). The DNA was then purified (PureLink PCR Micro kit; K310050) and the fragment ligated into the backbone

(8) OddCAPS: a simple, low-cost, universal technique for detecting single nucleotide variantsbioRxivJuly 16, 2026Karin Kawaguchi, Yuzuha Komachiya, Mai Muto, Ryoka Teshima, Naoko Sakai, Hayao Ohno(OddCAPS), primer synthesis (3 primers, total 94 nt) cost JPY 1,222 and EcoRI cost JPY 2,200 (NEB EcoRI-HF [R0101S], used at 2 units per sample), for a total of JPY 3,422 (≈ USD 21 / EUR 19 / CNY 144). Thus, in our

(9) Exosomal misfolded α1-antitrypsin triggers a cytosolic GRP78-dependent unfolded protein response and pro-survival signaling in pre-metastatic tissuesJournal of Biological ChemistryJune 20, 2026Anuran Bhattacharya, Tiyasa Saha, Aditya Shukla, Ananya Banerjee, Himansu Roy, Urmi ChatterjiCalcutta. T4 DNA ligase (catalog no.: M0202S), BamHI (catalog no.: R0136S) and EcoRI (catalog no.: R0101S) were obtained from New England BioLabs. Lipofectamine 2000 reagent (catalog no.: 11668019) was purchased

(10) ARID1A loss drives gastric signet ring cell carcinoma by regulating mucin production and secretionNature CommunicationsJune 2, 2026Hongyu Liu, Ailing Zhong, Zhenghao Lu, Limin Gao, Mengsha Zhang, Jiaxin Li, Yingjie Wang, Yiyun Wang, Lanxin Zhang, Jiabo Zhang, et al.obtained from the https://asia.ensembl.org/index.html, digested by BglII (Cat#R0144S) and EcoRI (Cat#R0101S) endonuclease from New England BioLabs, and followed by cloning into the retroviral plasmid MSCV-cDNA-IRES-GFP.

(11) CDK4 Mediates Cisplatin Resistance in Renal Cell Carcinoma (RCC) Cells by Regulating the ASH1L-CTR1 AxisOncology ResearchMay 21, 2026Wenjian Zeng, Xianglong Li, Hao Cai, Qingyu Zhou, Shuangshuang Sun, Pingping Li, Sunlong Li, Zhi Chenand the vector were subjected to double digestion with EcoR I (New England Biolabs Inc., Cat. No.: R0101S, Ipswich, MA, USA) and Xho I (New England Biolabs Inc., Cat. No.: R0146S, Ipswich, MA, USA), followed

(12) Elucidating the protein interaction network of one of the largest icosahedral capsids in the virosphereThe EMBO JournalApril 10, 2026Hela Safi, Alain Schmitt, Alwena Tollec, Lucid Belmudes, Agathe M G Colmant, Olivier Poirot, Sebastien Santini, Matthieu Legendre, Yohann Couté, Hugo Bisio, et al.Premix,Takara,639286 GoTaq,Promega,M3008 NotI-HF,New England Biolabs (NEB),R3189S EcoRI-HF,New England Biolabs (NEB),R0101S HindIII-HF,New England Biolabs (NEB),3104S SsoAdvanced Universal SYBR® Green Supermix,Bio-Rad,1725274 Prestained

(13) Generation of Nano-Antibodies Targeting the SNMP1 Membrane Receptor of Spodoptera littoralis Using Extracellular VesiclesJ Extracell BiolApril 7, 2026Alexis Dogliani, Laura Fradale, Céline Valeille, Christian Cambillau, Emmanuelle Jacquin-Joly, Alain Roussel, Josiane Kassis-Sahyounampicillin resistance, was digested with the restriction enzymes BglII (NEB #R0144S) and EcoRI (NEB #R0101S) for 1 h at 37°C. The linearized vector was then separated from the mCherry insert using 1% (w/v) agarose gel electrophoresis

(14) OSBPL3 promotes LUAD metastasis and glycolysis through transcriptional upregulation of PLAU via NFE2L2Translational OncologyApril 1, 2026Qingqiong Zhang, Jingshun Zhang, Junxian Li, Shuang Chen, Yijun Liu, Kaidi Li, Minzhang Guooligonucleotides was synthesized by Sangon Biotech (Shanghai). Restriction sites EcoRI (5′-GAATTC-3′; NEB #R0101L) and AgeI (5′-ACCGGT-3′; NEB #R3552L) were utilized for cloning. The annealed double-stranded oligonucleotides

(15) Dnmt3a-dependent de novo DNA methylation enforces lineage commitment and preserves functionality of memory Th1 and Tfh cellsCell ReportsMarch 24, 2026Bryant Perkins, Camille L. Novis, Andrew Baessler, Linda M. Sircy, Monyca M. Thomas, Malia Harrison-Chau, Andrew W. Richens, Bryce Fuchs, Nguyen X. Nguyen, Kaitlyn Flint, et al.Scientific,Catalog # 111456D AgeI-HF,New England Biolabs,Catalog #R3552S EcoRI,New England Biolabs,Catalog #R0101S Streptavadin-APC,Invitrogen,Catalog #S868 Influenza An NP 311–325 I-Ab QVYSLIRPNENPAHK APC,NIH Tetramer

(16) MASTR-seq enables multiplexed analysis of short tandem repeats with sequencingCell Rep MethodsMarch 23, 2026Chuanbin Su, Han-Seul Ryu, Keerthivasan Raanin Chandradoss, Thomas Malachowski, Ravi Boya, Linda Zhou, Hoa Emma Nguyen, Esteban O. Mazzoni, Kristen J. Brennand, Jennifer E. Phillips-Creminsfrom NEBNext Quick Ligation Module",New England Biolabs,Cat# E6056S EcoRI,New England Biolabs,Cat# R0101S BssH II,New England Biolabs,Cat# R0199S AP Detection buffer 10X (Alkaline Phos. buffer),Gene Link,Cat#

(17) The mechanism of ribosomal recruitment during translation initiation on the Type 2 encephalomyocarditis virus IRESThe EMBO JournalMarch 18, 2026Sayan Bhattacharjee, Irina S Abaeva, Zuben P Brown, Yani Arhab, Hengameh Fallah, Christopher U T Hellen, Joachim Frank, Tatyana V PestovaTranscriptase,Promega,M5108 T4 Polynucleotide Kinase,New England Biolabs,M0201S EcoRI,New England Biolabs,R0101S SpeI,New England Biolabs,R3133S BstNI,New England Biolabs,R0168S RNase T1,Thermo Fisher Scientific,EN0541 Isopropyl-beta-D-thiogalactoside,Gold Biotechnology

(18) Poly(A) variants supporting robust transmission stability in bacteria and high protein expression in animals for mRNA in vitro transcriptionMolecular Therapy Nucleic AcidsMarch 12, 2026Hua Chen, Wei Qin, Hui Bao, Xuwei Chen, Xu Ye, Yi Wang, Liang Liu, Yanguang Zhang, Ying Sun, Tingting Zhang, et al.respectively. The annealing product was then ligated into the pUC57 vector digested by EcoRI (NEB, Cat# R0101S) and SapI (NEB, Cat# R0569S) using T4 DNA ligase (NEB, Cat# M0202S). Positive clones were screened and

(19) CREB suppresses PGRP-SC2 to drive age-related immune senescence and gut dysbiosis in DrosophilaCell Death DiscoveryFebruary 24, 2026Saifei Wang, Bohan Qi, Peng Ma, Yao Zhang, Youjie Yin, Shuxin Chen, Hansong Dengligated into the pUAST vector using In-Fusion cloning. The vector cleavage site used was EcoRI (NEB, Cat#R0101S). Sequencing was performed to confirm the inserted sequence. Transgenic flies were generated using standard

(20) Molecular basis for anti-jumbo phage immunity by AVAST type 5Molecular CellFebruary 19, 2026Aswin Muralidharan, Ana Rita Costa, Desi Fierlier, Daan Frits van den Berg, Halewijn van den Bossche, Adja Damba Zoumaro-Djayoon, Alicia Rodríguez-Molina, Martin Pabst, Martin Pacesa, Bruno E. Correia, et al.SI-BG01 Dithiothreitol,Sigma-Aldrich,Cat# 43815-5G DpnI,New England Biolabs,Cat# R0176S EcoRI,New England Biolabs,Cat# R0101S FastAP,Fischer Scientific,Cat# EF0651 Glycerol,Fisher Scientific,Cat# 158920025 Isopropyl β-d-1-thiogalactopyranoside

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EcoRI

EcoRI from New England Biolabs

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New England Biolabs

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EcoRI from New England Biolabs

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