E. coliDNA Ligase from New England Biolabs

Product Specs
ItemE. coliDNA Ligase
CompanyNew England Biolabs
Price $271.00 Inquire
Catalog NumberM0205L
Concentration10,000 U/ml
Quantity1000 units
ApplicationsDNA Manipulation
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of lambda DNA in 30 minutes at 16°C.

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Self-resistance-gene-guided, high-throughput automated genome mining of bioactive natural products from StreptomycesCell SystemsMarch 19, 2025Yujie Yuan, Chunshuai Huang, Nilmani Singh, Guanhua Xun, Huimin ZhaoSynthesis Kit (E2040S), NAD+ (B9007S), dNTPs (N0447S), T4 DNA polymerase (M0203L), E. coli DNA ligase (M0205L), proteinase K (P8107S) for gDNA digestion, and restriction enzymes for BGC plasmid verification were

(2) Machine learning-optimized targeted detection of alternative splicingNucleic Acids ResearchJanuary 24, 2025Kevin Yang, Nathaniel Islas, San Jewell, Di Wu, Anupama Jha, Caleb M Radens, Jeffrey A Pleiss, Kristen W Lynch, Yoseph Barash, Peter S Choimixture containing a final concentration of 0.2 mM of each dNTP, 0.55 µl of 10 U/µl E. coli DNA Ligase (#M0205L, NEB), 2.08 µl of 10 U/µl E. coli DNA Polymerase I (#18010025, Invitrogen), 0.55 µl of 5 U/µl E. coli

(3) Multi-omics analysis reveals distinct gene regulatory mechanisms between primary and organoid-derived human hepatocytesDisease Models & MechanismsJanuary 1, 2025Haoyu Wu, Annie S. P. Yang, Suzan Stelloo, Floris J. M. Roos, René H. M. te Morsche, Anne H. Verkerk, Maria V. Luna-Velez, Laura Wingens, Johannes H. W. de Wilt, Robert W. Sauerwein, et al.60 min; 70°C for 10 min. Next, 960 nl second-strand reaction mix containing E. coli DNA ligase (NEB, M0205L), E. coli DNA polymerase I (NEB, M0209L) and random hexamer (Sigma-Aldrich, 11034731001) were added

(4) VarID2 quantifies gene expression noise dynamics and unveils functional heterogeneity of ageing hematopoietic stem cellsGenome BiologyJune 23, 2023Reyna Edith Rosales-Alvarez, Jasmin Rettkowski, Josip Stefan Herman, Gabrijela Dumbović, Nina Cabezas-Wallscheid, Dominic Grünsecond-strand buffer were replaced with E. coli DNA polymerase (NEB, M0209L), E. coli DNA ligase (NEB, M0205L), RNaseH (NEB, M0297S), and 10 × Second-Strand Buffer (NEB, B6117S) respectively. The water volume was

(5) T-RHEX-RNAseq – a tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cellsBMC GenomicsApril 17, 2023Charlotte Gustafsson, Julia Hauenstein, Nicolai Frengen, Aleksandra Krstic, Sidinh Luc, Robert Månssonenzymes were added (1U Sulfolobus DNA Polymerase IV NEB cat# M0327S and 10U E. coli DNA Ligase NEB cat# M0205L) and incubation continued for an additional 30 min at 37 °C. Samples were purified using AmPure XP beads

(6) Promoter‐pervasive transcription causes RNA polymerase II pausing to boost DOG1 expression in response to saltThe EMBO JournalMarch 1, 2023Miguel Montez, Maria Majchrowska, Michal Krzyszton, Grzegorz Bokota, Sebastian Sacharowski, Magdalena Wrona, Ruslan Yatusevich, Ferran Massana, Dariusz Plewczynski, Szymon SwiezewskiT40,Sigma‐Aldrich,31389‐500G dNTP Mix 10 mM,ThermoFisher,R0192 DTT,ThermoFisher,DTT001.10 E. coli DNA Ligase,New England Biolabs,M0205L E. coli DNA polymerase,New England Biolabs,M0209L EDTA Sterile Solution 0.5 M pH 8.0,BioShop,EDT111.500 Empigen,Sigma‐Aldrich,30326‐50ML Ethanol

(7) A single‐cell RNA labeling strategy for measuring stress response upon tissue dissociationMolecular Systems BiologyFebruary 10, 2023Anika Neuschulz, Olga Bakina, Victor Badillo‐Lisakowski, Pedro Olivares‐Chauvet, Thomas Conrad, Michael Gotthardt, Helmut Kettenmann, Jan Philipp JunkerSecond‐strand buffer (ThermoFisher #10812014), 0.25 μl dNTPs (10 mM total), 0.09 μl DNA ligase (New England Biolabs #M0205L), 0.35 μl E. coli DNA Pol I (New England Biolabs #M0209L), 0.09 μl RNase H (New England Biolabs #M0297L))

(8) Varying strength of selection contributes to the intragenomic diversity of rRNA genesNature CommunicationsNovember 25, 2022Daniel Sultanov, Andreas Hochwagen[Fisher #BP212R-1]), 2 μl 10 mM dA/G/C/UTPs (Promega #U1335), 0.5 μl 1U/μl E. coli DNA ligase (NEB #M0205L), 2 μl 10U/μl DNA Pol I (Enzymatics #P705L), and 0.5 μl 5U/μl RNase H (Enzymatics #Y922L). The mixture

(9) BCG Vaccination Induces Long-Term Functional Reprogramming of Human NeutrophilsCell reportsNovember 17, 2020Moorlag SJCFM, Rodriguez-Rosales YA, Gillard J, Fanucchi S, Theunissen K, Novakovic B, de Bont CM, Negishi Y, Fok ET, Kalafati L, et al.,New England Biolabs,Cat#M0209S USER enzyme,New England Biolabs,Cat#M5505L E.Coli Ligase,New England Biolabs,Cat#M0205L Rnasin Plus Rnase Inhibitor,Promega,Cat#N2615 Ribonuclease H,Life Technologies,Cat#AM2293 T4 DNA polymerase

(10) Transcriptome dynamics of the Myxococcus xanthus multicellular developmental programElifeOctober 14, 2019José Muñoz-Dorado, Aurelio Moraleda-Muñoz, Francisco Javier Marcos-Torres, Francisco Javier Contreras-Moreno, Ana Belen Martin-Cuadrado, Jared M Schrader, Penelope I Higgs, Juana PérezK Ambion Cat No./ID: 25530–015 Chemical compound, drug E. coli DNA ligase New England Biolabs Cat No./ID: M0205L Chemical compound, drug lysozyme Roche Cat No./ID: 10837059001 Commercial assay or kit RNeasy

(11) Transcriptome dynamics of the Myxococcus xanthus multicellular developmental programeLifeOctober 14, 2019José Muñoz-Dorado, Aurelio Moraleda-Muñoz, Francisco Javier Marcos-Torres, Francisco Javier Contreras-Moreno, Ana Belen Martin-Cuadrado, Jared M Schrader, Penelope I Higgs, Juana PérezK,Ambion,Cat No./ID: 25530–015, "Chemical compound, drug",E. coli DNA ligase,New England Biolabs,Cat No./ID: M0205L, "Chemical compound, drug",lysozyme,Roche,Cat No./ID: 10837059001, Commercial assay or kit,RNeasy

(12) Microscale Objects via Restructuring of Large, Double-Stranded DNA MoleculesACS Applied Materials & InterfacesDecember 5, 2018Samuel J. W. Krerowicz, Juan P. Hernandez-Ortiz, David C. Schwartznicks and gaps are removed through the addition of ten units of E. coli ligase (New England Biolabs, M0205L) and five units of E. coli polymerase I (Roche Applied Science, 10642720001) in the presence of a healing

(13) Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination systemPloS oneJanuary 1, 2018Szczesny RJ, Kowalska K, Klosowska-Kosicka K, Chlebowski A, Owczarek EP, Warkocki Z, Kulinski TM, Adamska D, Affek K, Jedroszkowiak A, et al.dUTP, 10 mM each, R0182, R0133, Thermo Fisher Scientific), water to 57 μl, 5 U E. coli DNA Ligase (M0205L, NEB), 20 U E. coli PLOS ONE | https://doi.org/10.1371/journal.pone.0194887 March 28, 2018 6 / 29 Streamlined

(14) β-Glucan Reverses the Epigenetic State of LPS-Induced Immunological ToleranceCellNovember 17, 2016Novakovic B, Habibi E, Wang SY, Arts RJW, Davar R, Megchelenbrink W, Kim B, Kuznetsova T, Kox M, Zwaag J, et al."DNA polymerase I, E. coli",New England Biolabs,M0209S USER enzyme,New England Biolabs,M5505L E.Coli Ligase,New England Biolabs,M0205L Rnasin Plus Rnase Inhibitor,Promega,N2615 Ribonuclease H,Life Technologies,AM2293 T4 DNA polymerase,New England Biolabs

(15) Genome-wide binding of transcription factors in inv(16) acute myeloid leukemiaGenomics dataDecember 1, 2014Mandoli A, Prange K, Martens JH.buffer, 40 U of Escherichia coli DNA polymerase (NEB # M02095S), 10 U of E. coli DNA ligase (NEB # M0205L) and 2 U of E. coli RNase H (Ambion # AM2293), and incubated at 16 °C for 2 h. This was followed by

(16) Changes in extreme cold tolerance, membrane composition and cardiac transcriptome during the first day of thermal acclimation in the porcelain crab Petrolisthes cinctipesJournal of Experimental BiologyJune 1, 2012Daria Ronges, Jillian P. Walsh, Brent J. Sinclair, Jonathon H. Stillmanpolymerase (M0209L, New England Biolabs). Following 2h incubation at 16°C, 1U E. coli DNA ligase (M0205L, New England Biolabs) and 11l 10⫻ DNA ligase buffer were added and incubated at room temperature for

Be the First to Write a Review!

Conversion of mRNA into Double- Stranded cDNA
January 01, 1995

E. coliDNA Ligase

E. coliDNA Ligase from New England Biolabs

Description

Specifications/Features

New England Biolabs

Citations (16)

Reviews

Protocols

Conversion of mRNA into Double- Stranded cDNA

E. coliDNA Ligase from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map