DpnII from New England Biolabs

Product Specs
ItemDpnII
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberR0543T
Concentration50,000 units/ml
Quantity1000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

5'-/GATC-3'

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) LINE-1 Locus Transcription Nucleates Oncogenic Chromatin ArchitectureCancer DiscoveryApril 1, 2026Michael Lee, Yuannyu Zhang, Jun Yi Stanley Lim, Tao Dai, James Ye, Margaret B. Cervantes, Varun Sondhi, Sisi Zheng, Yoon Jung Kim, Brandon Chen, et al.genomic DNA integrity, and the remaining samples were treated with 300 U DpnII (New England Biolabs, R0543M) overnight at 37°C with shaking at 2,000 rpm. After the overnight digestion period, another 10 µL was

(2) The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37Cell ReportsApril 1, 2026Joana R. Loureiro, Ana F. Castro, Ana S. Figueiredo, Ana Eufrásio, Ashutosh Dhingra, Mafalda Galhardo, Hugo Marcelino, Catarina C. Rodrigues, Paula Sampaio, Maria Azevedo, et al.(ND41866 and GM23280), as previously described30 with few modifications. The restriction enzymes DpnII (#R0543M, NEB) and Csp6I (#ER0211, Thermo Fisher Scientific) were used in the first and second restriction, respectively.

(3) A functional overlap between actively transcribed genes and chromatin insulator elementsThe EMBO JournalMarch 16, 2026Lucy J Cornell, Caroline L Harrold, Susannah Holliman, Felice Tsang, Matthew E Gosden, Lars L P Hanssen, Rosa Stolper, Damien J Downes, Daniel Biggs, Chris Preece, et al.screentape,Aligent,5067-5583 Tapestation RNA screentape,Aligent,5067-5576 DpnII,New England Biolabs,R0543M "T4 DNA Ligase, HC",Life Technologies Ltd,EL0013 Dynabeads M-280 Streptavidin,ThermoFisher,11205D NimbleGen SeqCap

(4) Multidimensional mapping of stimulation-responsive regulatory elements and candidate causal variants in T cell activationSci AdvJanuary 1, 2026Zhao Wang, Wei Dong, Xiangling Feng, Xinran Xu, Yao An, Huiling Xiao, Menghan Luo, Qian Liang, Ruoxuan Bai, Ke Zhao, et al.0.5% NP-40, 1% Triton X-100, and 1× protease inhibitors. Cells were then digested with Dpn II (NEB, R0543M) for 4 hours at 37°C while shaking at 1000g until the digestion efficiency exceeded 90%. Following digestion,

(5) Functional architecture of cardiac TF regulatory landscapes in control of mammalian heart developmentbioRxivDecember 23, 2025Virginia Roland, Johannes Tuechler, Andrea Esposito, Mattia Conte, Matteo Zoia, Ekapaksi Wisnumurti, Virginie Tissieres, Julie Gamart, Raquel Rouco Garcia, Ines J. Marques, et al.frozen in liquid nitrogen. 3C libraries were generated from thawed nuclei digested with DpnII (NEB, R0543M), followed by religation with T4 DNA ligase (Fisher Scientific) and subsequent de-crosslinking, as described

(6) Pre-established ATF4 occupancy and chromatin organization instruct selective transcription activation during integrated stress responseNat CommunOctober 28, 2025Peipei Jiang, Qian Biandigestion was carried out overnight at 37 °C by adding 50 μL of 10× NEB Buffer 2400 U of DpnII (NEB #R0543M), and 400 U of DdeI (NEB #R0175L). After digestion, restriction enzymes were inactivated by incubating

(7) Chromatin landscape, transcriptomic and ChIP-seq profiling of Anopheles stephensi MSQ43 cell lineScientific DataSeptember 26, 2025Varvara Lukyanchikova, Miroslav Nuriddinov, Anna Khabarova, Maria Gridina, Andrey Popov, Polina Belokopytova, Igor V. Sharakhov, Veniamin Fishmanwas replaced with fresh Neb 3.1 containing 1.8% triton X-100 and 400U of DpnII (New England Biolabs, R0543M) were added for overnight incubation. Next day, to improve restriction efficiency the reaction was supplemented

(8) Human papillomavirus genomes associate with active host chromatin during persistent viral infectionPLOS PathogensSeptember 2, 2025Alix Warburton, Tovah E. Markowitz, JJ L. Miranda, Kinjal Majumder, Alison A. McBrideInvitrogen, 15593–031) and chloroform (Macron Fine Chemicals) extractions and digested with 150U DpnII (NEB, R0543M) overnight at 37°C, with shaking at 900 rpm, prior to heat inactivation at 65°C for 20 min. Digested

(9) Xist upstream deletion leads to dysregulation of Xist and autosomal gene expressionGenome ResearchSeptember 2, 2025Sudeshna Majumdar, Lakshmi Sowjanya Bammidi, Hemant C. Naik, Avinchal Manhas, Runumi Baro, Akash Kalita, Amlan Jyoti Naskar, Sundarraj Nidharshan, Girija S. Bariha, Dimple Notani, et al.Proteinase K (Roche, #3115887001), PCR-purified, and ethanol-precipitated. Fifty units of DpnII (NEB, #R0543M) were used for the second restriction digestion, following which samples were again ligated, purified,

(10) NASP implication in the androgen receptor associated with castration resistance in prostate cancerCell communication and signaling : CCSJuly 10, 2025Feng Y, Sun J, Kang X, Wang Y, Liu K, Wang W, Wang C, Li Y, Zhao Q, Li K, et al.restriction enzymes, DdeI (New England Biolabs, cat. no. R0175L) and DpnII (New England Biolabs, cat. no. R0543M). The raw sequencing data were aligned to the human genome reference hg19 via Bowtie2 (version 2.3.4)

(11) Liebenberg syndrome severity arises from variations in Pitx1 locus topology and proportion of ectopically transcribing cellsNat CommunJuly 9, 2025Olimpia Bompadre, Raquel Rouco, Fabrice Darbellay, Antonella Rauseo, Fanny Guerard-Millet, Claudia Gentile, Marie Kmita, Guillaume Andreyal., 2019. In this process, chromatin underwent digestion with the DpnII enzyme (1000U total; NEB, R0543M) at 37 °C overnight, supplemented with 20% SDS and 20% Triton X-100. Subsequent ligation was carried

(12) Deletion of a single CTCF motif at the boundary of a chromatin domain with three FGF genes disrupts gene expression and embryonic developmentDevelopmental CellJuly 7, 2025Shreeta Chakraborty, Nina Wenzlitschke, Matthew J. Anderson, Ariel Eraso, Manon Baudic, Joyce J. Thompson, Alicia A. Evans, Lilly M. Shatford-Adams, Raj Chari, Parirokh Awasthi, et al.Cocktail",Millipore Sigma,Cat #11836170001 D1000 Screen Tape,Agilent,Cat #5067-5585 DpnII,New England Biolabs,Cat #R0543T "Proteinase K, recombinant, PCR Grade",Millipore Sigma,Cat #3115836001 "DNA Polymerase I, Large (Klenow) Fragment

(13) Brucella infection induces chromatin restructuring in host cells to activate immune responsesFront ImmunolJune 5, 2025Dejian Xie, Heling Xu, Changwei Su, Jingjing Lu, Wenlong Shen, Ping Li, Bingyu Ye, Jiabao Hou, Junwei Deng, Yan Zhang, et al.DdeI (New England Biolabs, Cat# R0175L) and 200U DpnII restriction enzymes (New England Biolabs, Cat# R0543M) for 16 hours. The digestion was terminated by incubating at 65°C for 20 minutes. The DNA ends were

(14) DNA G-quadruplex structures act as functional elements in α- and β-globin enhancersGenome BiolJune 4, 2025Colm Doyle, Krzysztof Herka, Sean M. Flynn, Larry Melidis, Somdutta Dhir, Stefan Schoenfelder, David Tannahill, Shankar BalasubramanianSigma-Aldrich) was added to each tube and incubated for 1 h at 37 °C at 950 rpm. Five hundred units of DpnII (R0543M, New England Biolabs) was added to each tube and incubated overnight at 37 °C. After centrifugation

(15) Rules of engagement for condensins and cohesins guide mitotic chromosome formationScienceApril 11, 2025Kumiko Samejima, Johan H. Gibcus, Sameer Abraham, Fernanda Cisneros-Soberanis, Itaru Samejima, Alison J. Beckett, Nina Puǎčeková, Maria Alba Abad, Christos Spanos, Bethan Medina-Pritchard, et al.10 minutes, quenched by 1% Triton X-100 (Sigma, 93443), and digested with 400 units of DpnII (NEB, R0543M) overnight at 37°C in a total volume of 475 μl. DpnII was inactivated at 65°C for 20 minutes. Digested

(16) Extensive mutual influences of SMC complexes shape 3D genome foldingNatureApril 10, 2025Han Zhao, Lirong Shu, Shiyi Qin, Fangxuan Lyu, Fuhai Liu, En Lin, Sijian Xia, Baiyue Wang, Manzhu Wang, Fengnian Shan, et al.to quench SDS. Restriction digestion was performed by adding 25U DpnII restriction enzyme (NEB, Cat#R0543M) and incubating the mixture at 37°C overnight with shaking (950rpm on a thermal mixer). After overnight

(17) TOP2B is required for compartment strength changes upon retinoic acid treatment in SH-SY5Y cellsChromosome ResApril 4, 2025Erica M. Hildebrand, Ian G. Cowell, Mushtaq M. Khazeem, Snehal Sambare, Ozgun Uyan, Job Dekker, Caroline A. AustinC. Flash-frozen cross-linked cells were thawed on ice for Hi-C, and were then lysed and digested with DpnII (NEB, R0543M) at 37 degrees C overnight, following the Hi-C 2.0 protocol (Belaghzal et al. 2017). The overhanging

(18) Identification of a SNAI1 enhancer RNA that drives cancer cell plasticityNat CommunMarch 25, 2025Chuannan Fan, Qian Wang, Peter H. L. Krijger, Davy Cats, Miriam Selle, Olga Khorosjutina, Soniya Dhanjal, Bernhard Schmierer, Hailiang Mei, Wouter de Laat, et al.After cell lysis and nuclei isolation, the samples were digested with 100 U DpnII (New England Biolabs, R0543M) and ligated using 50 U T4 DNA ligase (Thermo Fisher Scientific; EL0012). Next DNA samples were reverse

(19) Resolving the three-dimensional interactome of human accelerated regions during human and chimpanzee neurodevelopmentCellMarch 20, 2025Atreyo Pal, Mark A. Noble, Matheo Morales, Richik Pal, Marybeth Baumgartner, Je Won Yang, Kristina M. Yim, Severin Uebbing, James P. NoonanHiC protocol.29 Briefly, frozen nuclei pellets were thawed on ice, digested using 50U/ul DpnII (NEB # R0543M) at 37 °C overnight, followed by labeling with biotin-14 dATP (Thermofisher # 19524016) for 1 hr and

(20) Improved cohesin HiChIP protocol and bioinformatic analysis for robust detection of chromatin loops and stripesCommun BiolMarch 14, 2025Karolina Buka, Zofia Parteka-Tojek, Abhishek Agarwal, Michał Denkiewicz, Sevastianos Korsak, Mateusz Chiliński, Krzysztof H. Banecki, Dariusz Plewczynski(37 °C) incubation was carried out. Next, 75 μl of NEBuffer™ DpnII (NEB, B0543S) and 600U of DpnII (R0543M) were added to the sample and incubated for 1 h at 37 °C. To inactivate the restriction enzyme, the

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DpnII

DpnII from New England Biolabs

Description

Sequence

New England Biolabs

Citations (116)

Reviews

DpnII from New England Biolabs

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