DpnII from New England Biolabs

Product Specs
ItemDpnII
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberR0543S
Concentration10-50 u/µL
Quantity1000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

/GATC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Highly efficient chromatin conformation capture with post-enrichment in single cells by HiChewGenome BiolApril 27, 2026Zhichao Chen, Yeming Xie, Chen Zhang, Chen Tan, Fengying Ruan, Wenfang Chen, Meng Luo, Mei Guo, Zhe Xie, Huimin Cao, et al.for 15 min. Enzyme digestion and ligation For restriction digestion, 200 U of DpnII (NEB, Cat. No. R0543L) or AluI (NEB, Cat. No. R0137L) was added to the reaction and incubated at 37 °C overnight. Aliquots

(2) Stage-specific epigenetic priming amplifies gene activation during lineage commitmentSci AdvMarch 27, 2026Arion Foertsch, Naama Hirsch, Janine Seyfferth, Ward Deboutte, Herbert Holz, Maria Shvedunova, Remzi Karayol, Thomas Stehle, Laura Arrigoni, Niyazi Umut Erdogdu, et al.was incubated again (1 hour, 37°C, 900 rpm). The chromatin was digested using 300 U of Dpn II (NEB, R0543L) in three stages (100 U for 2 hours, 100 U overnight, and 100 U for 2 hours) at 37°C and 900 rpm. The

(3) Global genetic rewiring during compensatory evolution in the yeast polarity networkEMBO ReportsFebruary 16, 2026Enzo Kingma, Marieke Glazenburg, Karel Olavarria, Liedewij Laan,Thermo Fisher Scientific,AM9520 "Sodium Acetate, 3 M, pH 5.2, Molecular Biology Grade",Merck,567422 RNAse A,QIAGEN,19101 DpnII,NEB,R0543L NlaIII,NEB,R0125L T4 DNA Ligase (5 U/μL),Thermo Fisher Scientific,EL0011 Software,Software,Software Transposonmapper,PyPI,v1.1.4 Cytoscape,cytoscape.org,Version

(4) Effects of topological domain disruption on transcriptional regulation are chromatin context dependentEpigenetics & ChromatinFebruary 1, 2026Ángel Josué Cerecedo-Castillo, Diana Itzé Mojica-Santamaría, Hober Nelson Núñez-Martínez, Carlos Alberto Peralta-Alvarez, Gustavo Tapia-Urzúa, Georgina Guerrero, Rodrigo Gacel Arzate-Mejía, Félix Recillas-TargaIgepal) with 1 × protease inhibitors. First, three rounds of digestion were carried out with DpnII (NEB R0543S) at 37°C in a thermomixer at 500 rpm (100 U for 4 h, 100 U overnight, and 100 U for another 4 h); the

(5) Neural stem cell quiescence is actively maintained by the epigenomeCell ReportsJanuary 27, 2026Anna Malkowska, Jan Ander, Andrea H. Brand0.5M,Millipore,324506 Phosphate Buffer Saline (1×PBS),–,– RNAseA (DNAse free),Roche,11119915001 DpnI,NEB,R0176 DpnII,NEB,R0543S Klenow 3′–5′ exo-enzyme,NEB,M9212L Klenow Fragment (5 U/μL),NEB,M0210S MyTaq HS DNA Polymerase,Bioline,BIO-21113 NEBNext

(6) Pre-marking chromatin with H3K4 methylation is required for accurate zygotic genome activation and developmentNat CommunDecember 19, 2025Meghana S. Oak, Marco Stock, Ana Janeva, Matthias Mezes, Antony M. Hynes-Allen, Tobias Straub, Ignasi Forné, Andreas Ettinger, Stephan Hamperl, Axel Imhof, et al.M0202S; 2 h at 16 °C, heat inactivation at 65 °C for 20 min). Fragments were digested with DpnII (NEB, R0543S), purified with a 1:1.5 ratio of Seramag beads (Fisher Scientific, 65152105050250), and PCR amplified

(7) A chromosomal-level genome assembly of Thanatophilus sinuatus Fabricius (Coleoptera: Staphylinidae)Sci DataDecember 10, 2025Yingna Zhang, Liangliang Li, Shipeng Shao, Jianfeng Jin, Yu Wang, Ruixin Zhu5 min. The cross-linked cells were lysed, and isolated nuclei were digested with 80 U of MboI (NEB, R0543L) at 37 °C for 4 hours. The digested DNA was labeled with biotin-14-dCTP (Invitrogen) and ligated with

(8) Nanoscale dynamics of enhancer-promoter interactions during exit from pluripotencyNucleic Acids ResDecember 8, 2025Gabriela Stumberger, David Hörl, Dimitra Tsouraki, Clemens Steinek, A Marieke Oudelaar, Heinrich Leonhardt, Hartmann Harzmin and washed once with cold PBS. Restriction enzyme digestion was performed with 750 U DpnII (NEB, R0543S) at 37°C for 24 h. DpnII was subsequently inactivated at 65°C for 20 min and proximity ligation took

(9) Endogenous fine-mapping and prioritization of functional regulatory elements in complex genetic lociCell GenomNovember 12, 2025Zhao K, Zhou Y, Wang X, Wang Z, Xu X, Chen Y, Zhao L, Wu C, Wang J, Yao H, et al.Inhibitor Cocktail,Roche,Cat# C762Q77 BsmBI,New England Biolabs,Cat#R0580L DpnII,New England Biolabs,Cat#R0543L T4 DNA Ligase,New England Biolabs,Cat#M0202L CviQI,New England Biolabs,Cat#R0639L NlaIII,New England Biolabs

(10) Gene-Pseudogene Inversions as a Hidden Source of Missing HeritabilitymedRxivOctober 7, 2025Ilaria Quartesan, Stefano Facchini, Arianna Manini, Ricardo Parolin Schnekenberg, Chiara Pisciotta, Stefania Magri, Sara Negri, Carlo Gaetano, Adriana Rebelo, Jacquelyn Schatzman Raposo, et al.cells for fixation in 2% formaldehyde. Fixed cells were then lysed and digested using DpnII enzyme (R0543S, New England BioLabs, Ipswich, MA, USA). After digestion, restricted fragments were marked incorporating

(11) DNA methylation insulates genic regions from CTCF loops near nuclear speckleseLifeSeptember 3, 2025Shelby A Roseman, Allison P Siegenfeld, Ceejay Lee, Nicholas Z Lue, Amanda L Waterbury, Brian B Liauincubated at 37°C for 15 min. The samples were then digested with 375 units of DpnII (New England Biolabs, R0543L) for 2 hr at 37°C with shaking at 900 rpm, and then the enzyme was heat-inactivated at 65°C for 20 min.

(12) Single-stranded DNA-binding proteins are essential components of the architectural LDB1 protein complexMolecular CellAugust 21, 2025Xiaokang Wang, Nicholas G. Aboreden, Ying Cai, Jessica C. Lam, Kate A. Henderson, Jiaqi Xiang, Belinda M. Giardine, Ross C. Hardison, Cheryl A. Keller, Lalitha Nagarajan, et al.generate 4C templates, 10 million cells were fixed and processed as previously described91. DpnII (NEB, R0543L) and NlaIII (NEB, R0125L) were used as first and second restriction enzyme, respectively. The following

(13) β-catenin functions as a molecular adapter for disordered cBAF interactionsMolecular CellAugust 21, 2025Yuen San Chan, Qinyu Gao, Sarah A. Robinson, Wenzhi Wang, Ruzena Filandrova, Lisa-Maria Weinhold, Mario Loeza Cabrera, Miao Zhang, Chandra Shekar R. Ambati, Antonio M. Lerario, et al.2.5% Triton. DNA digestion and ligation Resuspended nuclei were digested with 100 U of DpnII (NEB #R0543S) at 37 °C for 3 hours, followed by an additional overnight incubation with another 100 U of DpnII. DpnII

(14) YTHDF3 recognizes DNA N6-methyladenine and recruits ALKBH1 for 6mA removal from genomic DNAThe EMBO JournalJuly 25, 2025Xin-Hui Chen, Zi-Lu Wang, Jincui Yang, Min Chen, Si-Yi Zhao, Kun-Xiong Guo, Xuelong Zheng, Zhengwei Zhao, Xiaoqiang Chen, Jing Li, et al.In brief, genomic DNA or 6mA-modified or unmodified dsDNA oligos were treated with DpnII (NEB, cat# R0543S) restriction enzyme or ddH2O at 25 °C overnight. The digested DNA and nondigested DNA were then purified

(15) Protocol for investigating chromatin architecture in adult skeletal muscle stem cells using high-throughput chromosome conformation captureSTAR ProtocJuly 21, 2025Jinjin Kang, Yaxin Zeng, Nianle Li, Liangqiang He, Huating Wang, Kun Sun, Yu ZhaoTriton X-100,Sigma-Aldrich,Cat# T8787 MgCl2 (1 M),Invitrogen,Cat# AM9530G DpnII,New England Biolabs,Cat# R0543L NEBuffer 3.1,New England Biolabs,Cat# B7203 dCTP,Takara,Cat# 4028 dGTP,Takara,Cat# 4027 dTTP,Takara,Cat#

(16) Klf5-adjacent super-enhancer functions as a 3D genome structure-dependent transcriptional driver to safeguard ESC identityNat CommunJuly 1, 2025Guangsong Su, Bohan Chen, Yingjie Song, Qingqing Yin, Wenbin Wang, Xueyuan Zhao, Sibo Fan, Jie Lian, Dongqing Li, Jinfang Bi, et al.light shaking) and centrifuged to remove supernatants. Nuclei were then digested with DpnII (NEB, ref. R0543L) at 37 °C. After inactivation for 20 min in 1.6% SDS at 65 °C, samples were diluted in 6.125 ml of 1.15×

(17) TAC-C uncovers open chromatin interaction in crops and SPL-mediated photosynthesis regulationSci AdvMay 30, 2025Jingmin Kang, Zhaoheng Zhang, Xuelei Lin, Fuyan Liu, Yali Song, Peng Zhao, Yujing Lin, Xumei Luo, Xiaoyi Li, Yanyan Yang, et al.library construction, the nuclei were resuspended in 500 μl 1.2× NEBuffer r2.1, and 40 μl of Dpn II (NEB, R0543L) was added. The reaction was carried out overnight at 37°C with constant agitation at 900 rpm in a metal

(18) JMJD1C forms condensates to facilitate a RUNX1-dependent gene expression program shared by multiple types of AML cellsProtein & CellMay 28, 2025Qian Chen, Saisai Wang, Juqing Zhang, Min Xie, Bin Lu, Jie He, Zhuoran Zhen, Jing Li, Jiajun Zhu, Rong Li, et al.treated with 0.5% SDS at 62°C for 5 min. Next, the nuclei were digested with 400 Units DpnII (NEB, R0543L) at 37°C three rounds and each round for 8–12 h. A 1/50 aliquot was removed as an “undigested control”

(19) Methyl-Micro-C: simultaneous characterization of chromatin accessibility, interactions, and DNA methylationNAR Genom BioinformMay 27, 2025Leonardo Gonzalez-Smith, Claire Stevens, Huan Cao, Zexun Wu, Suhn K Rhiebuffer and incubated on ice for 1 hour. Chromatin was then digested overnight with 100 U DpnII (NEB, R0543L) at 37°C. DpnII was inactivated, and the nuclei were cooled to room temperature for biotin fill-in.

(20) Condensin Accelerates Long-Range Intra-Chromosomal InteractionsbioRxivMay 25, 2025Fan Zou, Yi Li, Timothy Földes, Henrik Dahl Pinholt, Courtney Smith, Leonid Mirny, Lu Baicentrifugation, washed with 1 mL TBS, resuspended in 500 μL 10 mM Tris-HCl buffer and digested with DpnII (NEB, R0543L) overnight at 37 °C. Digested DNA fragments were filled in with biotin-labeled dATP by incubating with

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DpnII

DpnII from New England Biolabs

Sequence

New England Biolabs

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DpnII from New England Biolabs

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