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DAPI
DAPI
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DAPI Chemicals
DAPI from Santa Cruz Biotechnology, Inc.
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Citations:
(>1k)
Supplier Page
Supplier Page from
Santa Cruz Biotechnology, Inc. for
DAPI
Description
Fluorescent dye specific for DNA. Used to stain DNA chromosomes, nuclei for fluorescence microscopy. FACS, counterstain.
Binds preferentially to DNA rich in adenine (A) and thymidine (T).
Store at -20°C.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
DAPI
Company
Santa Cruz Biotechnology, Inc.
Price
$108.00
Supplier Page
View Company Product Page
Catalog Number
sc-3598
Quantity
10 mg
Type
Biochemical Reagents
Molecular Weight
350.24
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Santa Cruz Biotechnology, Inc.
10410 Finnell Street
Dallas
,
Texas
75220
United States
Phone
: 1-800-457-3801
831-457-3801
Company Profile
Website:
http://www.scbt.com/
Citations (1206)
(21)
Knocking down the neuronal lactate transporter MCT2 in the arcuate nucleus of female rats increases food intake and body weight
Sci Rep
March 3, 2025
Alanis Coca, Sergio López, Patricio Órdenes, Vania Sepúlveda, Diego Cuevas, Andrés Villarroel, Javiera Álvarez-Indo, Patricia V. Burgos, Estefanía Tarifeño, Roberto Elizondo-Vega,
et al.
111545003
and Cat#
703605155
respectively) and
DAPI
nuclear
staining
(
1:800
,
Santa Cruz Biotechnology
, Cat#
sc-3598
). Finally, the slices were mounted with Dako Fluorescent Mounting Medium (Dako Cytomation, Campintene,
(22)
Cholecystokinin facilitates the formation of long-term heterosynaptic plasticity in the distal subiculum
Commun Biol
February 1, 2025
Fengwen Huang, Abdul Baset, Stephen Temitayo Bello, Xi Chen, Jufang He
Anti-CCK BR (
Thermo Fisher
, Cat#
PA3-201
, RRID: AB_10979062);
DAPI
(
Santa Cruz Biotechnology
, Cat#
sc-3598
). Pro-CCK antibody (Frontier Institute, rabbit, RRID: AB_2571674). AAV
injection
Mice
were anesthetized
(23)
Saponin-based adjuvant uptake and induction of antigen cross-presentation by CD11b+ dendritic cells and macrophages
NPJ vaccines
January 22, 2025
Huis In 't Veld LG, Cornelissen LA, van den Bogaard L, Ansems M, Ho NI, Adema GJ.
washed with PBS, fixed with 4% PFA for 15 min at RT, washed in PBS and stained with DAPI (3 μg/ml,
sc-3598
,
Santa Cruz Biotechnology
) in PBS for 10 min at RT. Images were directly acquired (without mounting)
(24)
The CXCR4 antagonist R54 targets epithelial-mesenchymal transition (EMT) in human ovarian cancer cells
PLOS ONE
December 19, 2024
Daniela Russo, Anna Spina, Luigi Portella, Anna Maria Bello, Francesca Galdiero, Anna Maria Trotta, Caterina Ieranò, Giuseppina Rea, Sabrina Chiara Cecere, Elisabetta Coppola,
et al.
surface of the insert were fixed in 4% (w/v) PFA in PBS and stained for 15 min with
DAPI
(
1:25
.000,
sc-3598
Santa Cruz
). Cells were visualized with a
fluorescent microscope
(Carl Zeiss, Axio Scope.A1) and counted
(25)
Mechanical Stretch Control of Adipocyte AKT Signaling and the Role of FAK and ROCK Mechanosensors
Bioengineering (Basel)
December 16, 2024
Tasneem Bouzid, Eunju Kim, Brandon D. Riehl, Ruiguo Yang, Viswanathan Saraswathi, Jason K. Kim, Jung Yul Lim
Invitrogen
, Carlsbad, CA, USA), LipidSpot (
1:1000
,
70069
,
Biotium
, Fremont, CA, USA), and
DAPI
(
1:1000
,
sc-3598
,
Santa Cruz
, Dallas, TX, USA). To detect
GLUT4
, an
immunofluorescence analysis
was performed using a
(26)
Flexible adjustment of oxytocin neuron activity in mouse dams revealed by microendoscopy
Science Advances
December 13, 2024
Kasane Yaguchi, Kazunari Miyamichi, Gen-ichi Tasaka
solution; and 15 min in 4′,6-diamidino-
2-phenylindole
(2.5 μg/ml;
Santa Cruz Biotechnology
, catalog no.
sc-3598
) in PBS. Sections were mounted on slides and cover-slipped with mounting medium (Fluoromount, Diagnostic
(27)
Acer tegmentosum Maxim and Bacillus subtilis-fermented products inhibit TNF-α-induced endothelial inflammation and vascular dysfunction of the retina: the role of tyrosol moiety in active compounds targeting Glu230 in SIRT1
Frontiers in Pharmacology
November 20, 2024
Phuc Anh Nguyen, Jong Soon Won, Min Kyung Cho
molecule-1 (VCAM-1) (
sc-8304
) antibodies, anti-IgG-FITC (
sc-2010
), protein G agarose (
sc-2002
),
DAPI
(
sc-3598
), and
Immuno In situ Mount
(
sc-45088
) were obtained from
Santa Cruz Biotechnology
(CA, United States).
(28)
The therapeutic use of clonal neural stem cells in experimental Parkinson´s disease
Stem cell research & therapy
October 9, 2024
Nelke A, García-López S, Caso JR, Pereira MP.
incubated with adequate secondary antibodies and 4′,6-diamidino-
2-phenylindole
(
1:1000
;
Santa Cruz
sc-3598
), in 1% serum in 1X PBS/0.3% TritonX-100 at RT for 2 h. The following secondary antibodies were used:
(29)
Characterizations of Protein Arginine Deiminase 1 as a Substrate of NTMT1: Implications of Nα-Methylation in Protein Stability and Interaction
Journal of Proteome Research
October 4, 2024
Ying Meng, Zhouxian Li, Ming He, Quanqing Zhang, Youchao Deng, Yinsheng Wang, Rong Huang
at room temperature for 2 h. Finally, the nuclei were stained with
DAPI
(
Santa Cruz Biotechnology
#
sc-3598
). Images were captured using a Lionheart FX microscope with a 40× objective.
Cycloheximide
(CHX) chase
(30)
Monoamine Oxidase Contributes to Valvular Oxidative Stress: A Prospective Observational Pilot Study in Patients with Severe Mitral Regurgitation
International Journal of Molecular Sciences
September 25, 2024
Raluca Șoșdean, Maria D. Dănilă, Loredana N. Ionică, Alexandru S. Pescariu, Monica Mircea, Adina Ionac, Cristian Mornoș, Constantin T. Luca, Horea B. Feier, Danina M. Muntean,
et al.
as previously described [24]. Nuclear
staining
was obtained with
DAPI
(
Santa Cruz
, Dallas, TX, USA,
SC3598
). The slides were examined using an Olympus Fluoview FV1000
confocal
microscope (Hachioji, Tokyo, Japan).
(31)
Human papillomavirus 16 replication converts SAMHD1 into a homologous recombination factor and promotes its recruitment to replicating viral DNA
Journal of Virology
September 17, 2024
Claire D. James, Aya Youssef, Apurva T. Prabhakar, Raymonde Otoa, Jenny D. Roe, Austin Witt, Rachel L. Lewis, Molly L. Bristol, Xu Wang, Kun Zhang,
et al.
(Molecular Probes). Cellular DNA was stained with 4’,6-diamidino-
2-phenylindole
(
DAPI
,
Santa Cruz
,
sc-3598
). The coverslips were mounted onto slides using Vectashield medium (Thermo Fisher) and were visualized,
(32)
Impact of Uniaxial Static Strain on Myoblast Differentiation in Collagen-Coated PCL Microfilament Scaffolds: Role of Onset Time of Mechanical Stimulation
Bioengineering (Basel, Switzerland)
September 13, 2024
Espinoza-Álvarez ML, Rojas-Rojas L, Morales-Sánchez J, Guillén-Girón T.
BP9700-100), 4′, 6-diamidino-
2-phenylindole
(
DAPI
) (
Santa Cruz Biotechnology
, Dallas, TX, USA, Catalog No.
sc-3598
, CAS No. 28718-90-3),
Alexa Fluor 488 Phalloidin
(
Invitrogen
by Thermo Fisher Scientific, Catalog No.
(33)
Wound Closure Promotion by Leucine-Based Pseudo-Proteins: An In Vitro Study
International Journal of Molecular Sciences
September 6, 2024
Mariam Ksovreli, Tinatin Kachlishvili, Mariam Skhvitaridze, Lili Nadaraia, Rusudan Goliadze, Luka Kamashidze, Knarita Zurabiani, Tatuli Batsatsashvili, Nino Kvachantiradze, Marekhi Gverdtsiteli,
et al.
(Carl Roth GmbH, Art.-Nr. 0335.1, Karlsruhe, Germany), 4′,6-diamidino-
2-phenylindole
(
DAPI
) (Cat. No.
SC-3598
),
dimethyl sulfoxide (DMSO
) (Cat. No.
SC 202581A
), phosphate-buffered saline (PBS) (Cat. No.
SC-24947
),
(34)
Sex-dependent regulation of retinal pigment epithelium and retinal function by Pgc-1α
Frontiers in Cellular Neuroscience
September 2, 2024
Kaan Taskintuna, Mohd Akbar Bhat, Tasneem Shaikh, Jacob Hum, Nady Golestaneh
incubated for 1.5 h at room temperature, along with counterstains
DAPI
for nuclei (
1:1000
dilution;
sc-3598
;
Santa Cruz Biotechnology
, Santa Cruz, CA, United States),
Texas Red-X Phalloidin
for cytoskeletal
actin filaments
(35)
Creg1 Regulates Erythroid Development via TGF-β/Smad2-Klf1 Axis in Zebrafish
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
September 1, 2024
Han X, He W, Liang D, Liu X, Zhou J, de Thé H, Zhu J, Yuan H.
(
Thermo Fisher Scientific
,
BMS500FI‐300
) for 10 min in the dark at room temperature. Then 1 µL
DAPI
(
Santa Cruz
,
sc‐3598
) was added to the samples immediately before the
flow cytometric analysis
. Data acquisition was performed
(36)
Methylene blue reduces monoamine oxidase expression and oxidative stress in human cardiovascular adipose tissue
Molecular and Cellular Biochemistry
August 21, 2024
Oana-Maria Aburel, Laurențiu Brăescu, Darius G. Buriman, Adrian P. Merce, Anca M. Bînă, Claudia Borza, Cristian Mornoș, Adrian Sturza, Danina M. Muntean
anti-rabbit antibody Alexa Fluor labeled (
Invitrogen
,
A32731
). Nuclei were stained with
DAPI
(
Santa Cruz
,
SC3598
). The slides were analyzed in
confocal microscopy
(Olympus Fluoview FV1000
confocal
microscope) by means
(37)
CXCL12-loaded-hydrogel (CLG): A new device for metastatic circulating tumor cells (CTCs) capturing and characterization
Heliyon
August 15, 2024
Portella L, Bertolini G, Guardascione G, Di Febbraro DG, Ieranò C, D'Alterio C, Rea G, Napolitano M, Santagata S, Trotta AM,
et al
.
the
membrane
insert were fixed in 4 % (w/v) paraformaldehyde in PBS and stained with
DAPI
(
1:25
.000,
sc-3598
Santa Cruz
for 15min). Cells were imaged fluorescent microscope (Carl Zeiss, Axio Scope.A1) and counted
(38)
Empagliflozin and dapagliflozin decreased atrial monoamine oxidase expression and alleviated oxidative stress in overweight non-diabetic cardiac patients
Molecular and Cellular Biochemistry
July 23, 2024
Loredana N. Ionică, Darius G. Buriman, Adina V. Lința, Raluca Șoșdean, Ana Lascu, Caius G. Streian, Horea B. Feier, Lucian Petrescu, Ioana M. Mozoș, Adrian Sturza,
et al.
Alexa Fluor-labeled secondary
goat
anti-rabbit antibody (
Invitrogen
,
A32731
), while
DAPI
(
Santa Cruz
,
SC3598
) was used for the nuclear
staining
. The slides were examined in
confocal microscopy
(Olympus Fluoview
(39)
Knockout of the longevity gene Klotho perturbs aging and Alzheimer’s disease-linked brain microRNAs and tRNA fragments
Communications Biology
June 11, 2024
Serafima Dubnov, Estelle R. Bennett, Nadav Yayon, Or Yakov, David A. Bennett, Sudha Seshadri, Elliott Mufson, Yonat Tzur, David Greenberg, Makoto Kuro-o,
et al.
min
incubation
. Re-pelleted cells were resuspended in
staining
buffer with
DAPI
(
1:1000
;
Santa Cruz
,
sc-3598
). All steps were performed on ice.
Iba1
- and NeuN-positive cells were sorted through a 85 μm nozzle
(40)
Helicobacter pylori disrupts gastric mucosal homeostasis by stimulating macrophages to secrete CCL3
Cell Communication and Signaling
May 10, 2024
Yan-Fei Wei, Xue Li, Meng-Ran Zhao, Si Liu, Li Min, Sheng-Tao Zhu, Shu-Tian Zhang, Si-An Xie
594 was added and incubated for 1 h at room temperature. Finally, the nuclei were stained with
DAPI
(
sc3598
,
Santa Cruz
, USA), and the cells were observed under a
confocal
microscope (
Olympus
FluoView™
FV1200
,
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Reviews
Be the First to Write a Review!
DAPI from Santa Cruz Biotechnology, Inc.
Supplier Page
View Company Product Page
$108.00
Product Specs
Company Info
Citations
Reviews
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