UNICORN™ 7 control software from Cytiva

Product Specs
ItemUNICORN™ 7 control software
CompanyCytiva Life Sciences
Price Get Pricing Get Quote for this Product
Catalog NumberUNICORN 7
QuantityEA
Electrical Requirements300 VA

Cytiva Life Sciences

(1) Transcriptomics analysis identifies folding and secretion related genes for improving monoclonal antibody production in Thermothelomyces heterothallica C1Microbial Cell FactoriesApril 2, 2026Mari Mäkinen, Antti Aalto, Tiina Pakula, Marilyn G. Wiebe, Anne Huuskonen, Marika Vitikainen, Mari Valkonen, Sami Havukainen, Ellinor Englund, Veera Korja, et al.purified mAb was quantified based on absorbance at 280 nm by integrating the elution peak with the Unicorn 7 software (Cytiva, USA). RNA extraction Total RNA was isolated using TRIzol reagent (Thermo Fisher Scientific

(2) Immunogenicity and protective efficacy of MERS CoV, NL140422, and HKU4 spike protein nanoparticle vaccinesnpj VirusesFebruary 19, 2026Peter J. Halfmann, Jeong Soo Lee, Tong Wang, Augustine Duffy, Yoshihiro Kawaoka, Ravi S. Kanecontained 8 μg of S. The solution was run on Superdex 200 Increase 10/300 Column (Cytiva) using the Unicorn 7 control system (Cytiva) and was eluted with 1 CV of PBS at 0.65 mL/minute. The absorbance at 210 nm

(3) On-Column Dual-Gradient Refolding for Efficient Recovery of Insoluble Affinity-Tagged Recombinant ProteinsBio ProtocFebruary 5, 2026Anna Vlaskina, Dmitry Petrenko, Yulia Agapova, Anastasia Kuzminkova, Marta Evteeva, Maxim Patrushev223TV) 24. Refrigerator (2–8 °C) ХЛ-250 POZIS (Pozis, catalog number: 223TV) Software and datasets 1. UNICORN 7 control software (Cytiva) 2. Image Lab Software (Bio-Rad) Procedure A. Expression of recombinant proteins Note: In

(4) On-Column Dual-Gradient Refolding for Efficient Recovery of Insoluble Affinity-Tagged Recombinant ProteinsBIO-PROTOCOLFebruary 5, 2026Anna Vlaskina, Dmitry Petrenko, Yulia Agapova, Anastasia Kuzminkova, Marta Evteeva, Maxim Patrushev223TV) 24. Refrigerator (2–8 °C) ХЛ-250 POZIS (Pozis, catalog number: 223TV) Software and datasets 1. UNICORN 7 control software (Cytiva) 2. Image Lab Software (Bio-Rad) Procedure A. Expression of recombinant proteins Note: In

(5) Mechanisms of HSV-1 helicase-primase inhibition and replication fork complex assemblyCellJanuary 22, 2026Zishuo Yu, Pradeep Sathyanarayana, Cong Liu, Joel M.J. Tan, Pan Yang, Biswajit Das, Side Hu, Xiaoyi Fan, Chenggong Ji, Sandra K. Weller, et al.RRID:SCR_002798" UNICORNTM version 7.8,Cytiva,https://www.cytivalifesciences.com/en/us/shop/chromatography/software/unicorn-7-p-05649 AlphaFold Server,"Abramson et al., 202441","https://deepmind.google/technologies/alphafold/alphafold-server/,

(6) Targeted computational design of an interleukin-7 superkine with enhanced folding efficiency and immunotherapeutic efficacyeLifeJanuary 16, 2026See-Khai Lim, Wen-Ching Lin, Yi-Chung Pan, Sin-Wei Huang, Yao-An Yu, Cheng-Hung Chang, Che-Ming Jack Hu, Chung-Yuan Mou, Kurt Yun Mouand (D) Neo-7-Q6P-T45I. Percentage of purity is calculated from the SEC-FPLC peak profile via Cytiva Unicorn 7 software after affinity chromatography purification. SPR (Biacore) characterization of the binding kinetics

(7) Targeted computational design of an interleukin-7 superkine with enhanced folding efficiency and immunotherapeutic efficacyElifeJanuary 16, 2026See-Khai Lim, Wen-Ching Lin, Yi-Chung Pan, Sin-Wei Huang, Yao-An Yu, Cheng-Hung Chang, Che-Ming Jack Hu, Chung-Yuan Mou, Kurt Yun Mouand (D) Neo-7-Q6P-T45I. Percentage of purity is calculated from the SEC-FPLC peak profile via Cytiva Unicorn 7 software after affinity chromatography purification. SPR (Biacore) characterization of the binding kinetics

(8) De novo design of protein nanoparticles with integrated functional motifsbioRxivJanuary 5, 2026Cyrus M. Haas, Sanela Rankovic, Hanul K. Lewis, Kenneth D. Carr, Connor Weidle, Sophie S. Gerdes, Lily R. Nuss, Felicitas Ruiz, Syed Moiz, Maggie Fiorelli, et al.500 mM NaCl, 100 mM L-Arginine). Instrument control and elution profile analysis were performed using UNICORN 7 software (Cytiva). HEK293F expression and purification Plasmids for mammalian expression were purified

(9) A novel chromatography method for high purity production of Recombinant human parathyroid hormone from E. coliScientific ReportsDecember 30, 2025Farhad Mollasoltani, Milad Adak, HomaSadat Esfahani, Elaheh Roshani Yasaghi, Sirwan Khanchezarwith Unicorn 7.0 version software (https://www.cytivalifesciences.com/en/us/shop/cell-therapy/software/unicorn-7-p-05649?psmenu=0, Cytiva, Uppsala, Sweden). Capto™ Adhere Impres resin (Catalog Number: [17371502],

(10) Molecular characterisation of the Bacillus subtilis SpbK antiphage defence systemNature CommunicationsDecember 29, 2025Biswa P. Mishra, Christian L. Loyo, Yanyao Cai, Thomas Litfin, Gause Miraj, Lou Brillault, Veronika Masic, Tamim Mosaiab, Premraj Rajaratnam, Santosh Rudrawar, et al.Pure device (Cytiva) at 0.5 mL/min. Analysis of chromatograms and image preparation were performed in Unicorn 7 software (Cytiva) and Excel (Microsoft). Peak fractions were analysed by SDS-PAGE. Mass photometry Mass

(11) From sequence to scaffold: Computational design of protein nanoparticle vaccines from AlphaFold2-predicted building blocksProceedings of the National Academy of SciencesNovember 11, 2025Cyrus M. Haas, Naveen Jasti, Annie Dosey, Joel D. Allen, Rebecca Gillespie, Jackson McGowan, Elizabeth M. Leaf, Max Crispin, Cole A. DeForest, Masaru Kanekiyo, et al.25 mM Tris pH 8.0, 150 mM NaCl. Instrument control and elution profiles analysis were performed with UNICORN 7 software (Cytiva). Mammalian Protein Expression and Purification. For purification of plasmid DNA

(12) Structural basis of apoptosis induction by the mitochondrial voltage-dependent anion channelNat CommunOctober 27, 2025Melina Daniilidis, Umut Günsel, Georgios Broutzakis, Kira D. Leitl, Robert Janowski, Kai Fredriksson, Dierk Niessing, Christos Gatsogiannis, Franz HagnNi-NTA and a HiLoad 16/600 Superdex 200 pg column (Cytiva) on an Äkta Purifier system controlled by Unicorn 7 software. For the preparation of nanodiscs for cryo-EM, the size-exclusion chromatography buffer was

(13) Production of Homogeneous, Functional Zinc-Finger Arrays in High Yield With Two Chromatographic StepsBio ProtocAugust 20, 2025Jingchang Liang, Maia Azubel, Guanqiao Wang, Yan Nie, Roger D. Kornberg, Andrew J. Beel, Pierre-Jean MatteïÄKTA purifier (GE Healthcare, https://www.cytivalifesciences.com/en/us/shop/chromatography/software/unicorn-7) Procedure A. Transformation of E. coli BL21 (DE3) with ZF-array expression plasmids 1. DNA fragments

(14) Production of Homogeneous, Functional Zinc-Finger Arrays in High Yield With Two Chromatographic StepsBio ProtocAugust 20, 2025Jingchang Liang, Maia Azubel, Guanqiao Wang, Yan Nie, Roger D. Kornberg, Andrew J. Beel, Pierre-Jean MatteïÄKTA purifier (GE Healthcare, https://www.cytivalifesciences.com/en/us/shop/chromatography/software/unicorn-7) Procedure A. Transformation of E. coli BL21 (DE3) with ZF-array expression plasmids 1. DNA fragments

(15) De Novo Design of High-Performance Cortisol Luminescent BiosensorsJournal of the American Chemical SocietyAugust 6, 2025Julie Yi-Hsuan Chen, Xue Peng, Chenggang Xi, Gyu Rie Lee, David Baker, Andy Hsien-Wei Yehwithout further purification. Size exclusion chromatography was performed using a KTA pure M system with UNICORN 7 Workstation control (GE Healthcare) coupled with a Superdex 75 Increase 10/300 GL column. DNA and protein

(16) DNA-encoded library screening uncovers potent DNMT2 inhibitors targeting a cryptic allosteric binding siteiScienceAugust 5, 2025Ariane F. Frey, Merlin Schwan, Annabelle C. Weldert, Valerie Kadenbach, Jürgen Kopp, Zarina Nidoieva, Robert A. Zimmermann, Lukas Gleue, Collin Zimmer, Marko Jörg, et al.compound 3. The resulting chromatograms were analyzed by superposing their 280 nm signals using the Unicorn7 evaluation software (Cytiva). Crystallization, data collection, model building, and refinement Crystals

(17) Teicoplanin Nonribosomal Peptide Synthetase Is Unable to Incorporate Alpha-Ketoacid Building BlocksBiochemistryMay 6, 2025Minuri S. Ratnayake, Xinyun Jian, Julien Tailhades, Gregory L. Challis, Mathias H. Hansen, Józef R. Lewandowski, Max J. Crylecrude, 5 mL, Cytiva) pre-equilibrated in lysis buffer using an Akta Start Purification System (running Unicorn 7, Cytiva). The target protein was eluted in fractions with a linear gradient from 10 mM to 300 mM imidazole

(18) Antibody Purification Using Spy ChemistryBio ProtocApril 20, 2025Xiaofeng Yang, Zhanglin Lin, Ya Xiang, Zisha LaoAKTA purifier (GE Healthcare, https://www.cytivalifesciences.com/en/us/shop/chromatography/software/unicorn-7-p-05649 ) Procedure A. Protein expression 1. Transform E. coli BL21(DE3) bacterial strain with the

(19) A Robust and Easy Protein Purification Method Using SpyDock-Modified ResinBio ProtocApril 20, 2025Xiaofeng Yang, Zhanglin Lin, Ya Xiang, Binrui Chen, Zisha LaoAKTA purifier (GE Healthcare, https://www.cytivalifesciences.com/en/us/shop/chromatography/software/unicorn-7-p-05649) Procedure Note: Perform most procedures at room temperature (25 °C), except for steps where

(20) Antibody Purification Using Spy ChemistryBio ProtocApril 20, 2025Xiaofeng Yang, Zhanglin Lin, Ya Xiang, Zisha LaoAKTA purifier (GE Healthcare, https://www.cytivalifesciences.com/en/us/shop/chromatography/software/unicorn-7-p-05649 ) Procedure A. Protein expression 1. Transform E. coli BL21(DE3) bacterial strain with the

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