Description
The Cytiva fibro dT is an adsorber of electrospun cellulose fiber functionalized with dT oligonucleotides designed to isolate RNA with polyA tail from crude sample. The fibro dT 0.4 mL unit is developed for high binding capacity of the target mRNA and for rapid chromatographic cycles. The intended use is with ÄKTA chromatography systems in RNA research laboratories.
Rapid chromatography cycle time
Less than 15 minutes incl. cleaning
High capacity
Over 6 mg/mL is repeatedly observed for 2000-mer mRNA
High reproducibility
Over at least 20 cycles and between different fibro dT batches
These ready-to-use units are designed for research. They are suitable for screening and optimization of process conditions. The housing is made of polypropylene plastic with stoppers at the inlet and outlet. Find more information in the data file found in literature section below.
The open structure of the fibro adsorber enables high mass transfer at high flow rates. In addition, the fibro properties allow for high binding capacity, particularly in vitro transcribed RNA longer than 1000 nucleotides. The RNA must have a polyA-tail to bind complementary to the fibro dT adsorber.