Description
Activated Thiol Agarose Fast Flow medium is a medium used for reversible immobilization of molecules containing thiol groups under mild conditions. Optimized for immobilization of large molecules. Activated Thiol Agarose Fast Flow reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for immobilizing thiol containing biomolecules1. Reversible coupling of proteins and large biomolecules with thiol groups via a glutathione spacer arm2. Well suited for covalent chromatography of large molecules such as enzymes and nucleic acids3. Gel also reacts with heavy metal ions and with alkyl and aryl halides. Addition reactions occur with compounds containing C=O, C=C, and N=N bonds4. Separate thiol-containing proteins from non-thiol-containing proteins