Description
Butyl Cellulose is useful for the chromatography of hydrophobic proteins. Many proteins have hydrophobic amino acid residues which will interact with the phenyl, butyl and octyl functional groups. Factors that influence this hydrophobic interaction include salt concentration, temperature, pH, organic solvents and surfactants. Protein adsorption usually occurs at high ionic strength, while elution occurs at lower salt concentrations. This is the opposite of ion exchange chromatography and complementary