Macro-Prep DEAE Support from Bio-Rad

Product Specs
ItemMacro-Prep DEAE Support
CompanyBio-Rad
Price $53.00Supplier Page View Company Product Page
Catalog Number1580020

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Bacteriophage SPO1 protein Gp46 suppresses functions of HU protein in Francisella tularensisFront MicrobiolDecember 8, 2023Petra Spidlova, Eliska Sokolova, Pavla Pavlikpossibility of E. coli HU protein co-purification, anion exchange chromatography was performed using Macro-Prep DEAE Support (Bio-Rad, 1560020). The concentration of the protein samples was determined using a Qubit assay (Invitrogen,

(2) Ferritin microheterogeneity, subunit composition, functional, and physiological implicationsScientific reportsNovember 14, 2023Srivastava AK, Reutovich AA, Hunter NJ, Arosio P, Bou-Abdallah F.applied to a DEAE column (25 ml Macro-Prep DEAE, 50 μm particle size, 10 ml column volume, Catalog no 1580020 from Bio-Rad Laboratories, Inc.) equilibrated with 20 mM Tris buffer, pH 7.4. The elution buffer was

(3) Biochemical characterization and thermal inactivation of polyphenol oxidase from elephant foot yam (Amorphophallus paeoniifolius)Journal of Food Science and TechnologyJune 1, 2017Anuradha Singh, Neeraj Wadhwa(sodium dodecyl sulphate), DTT (dithiothreitol) were procured from Sigma Chemical Co (St. Louis, USA). Macro-prep DEAE support of Biorad was used for purification of enzyme. All reagents used were of analytical grade. Preparation

(4) Characterization of recombinant human lysosomal beta-hexosaminidases produced in the methylotrophic yeast Pichia pastorisUniversitas ScientiarumSeptember 29, 2016Angela Johana Espejo Mojica, Angela Rocío Mosquera, Edwin Alexander Rodríguez-López, Dennis Johana Díaz, Laura Milena Betrán, Francy Liliana Hernández, Carlos Javier Alméciga Díaz, Luis Alejandro BarreraBio-Rad Laboratories), using Q-Sepharose Fast Flow column (Amersham Biosciences) for rhHex-S, Q-Sepharose Fast Flow or Macro-Prep DEAE Support (Bio-Rad Laboratories) for rhHex-A, while rhHex-B was purified by using Macro-Prep High S Support. Protein

(5) Mutational and Computational Evidence That a Nickel-Transfer Tunnel in UreD Is Used for Activation of Klebsiella aerogenes UreaseBiochemistryOctober 20, 2015Mark A. Farrugia, Beibei Wang, Michael Feig, Robert P. Hausinger100,000 g for one h at 4 °C. Supernatants were diluted 1:1 in TEB buffer before being applied to a 90 mL Macro-Prep® DEAE Support (Bio-Rad) column pre-equilibrated in TEB buffer. The samples were washed with one volume of TEB buffer

(6) Structureâ€“function relationships for Schizophyllum commune trehalose phosphorylase and their implications for the catalytic mechanism of family GT-4 glycosyltransferasesBiochemical JournalAugust 1, 2006Christiane Goedl, Richard Griessler, Alexandra Schwarz, Bernd Nidetzkysupernatant (∼ 15 ml containing 450 mg of protein) was applied on to an XK 26/40 column packed with 50 ml of Macro-Prep DEAE Support gel (Bio-Rad) and equilibrated with 50 mM potassium phosphate buffer (pH 7.0). (Note that isolation

(7) Cell surface heparan sulfate proteoglycans contribute to intracellular lipid accumulation in adipocytesliphdisJanuary 6, 2005Larissa C Wilsie, Shree Chanchani, Deepti Navaratna, Robert A Orlandowas removed by centrifugation (10,000 × g, 4°C, 5 min) and supernatant was batch adsorbed onto 0.5 ml Macro-Prep DEAE Support (BioRad) (pre-washed with solubilization buffer) with gentle agitation for 1 h at 4°C. DEAE resin was

(8) Expanding the Functional Repertoire of CTD Kinase I and RNA Polymerase II: Novel PhosphoCTD-Associating Proteins in the Yeast ProteomeBiochemistryDecember 21, 2004Hemali P. Phatnani, Janice C. Jones, Arno L. Greenleafequivalent to that of 0.2 M (NH4)2SO4. This material was then passed through a DEAE column (200 mL, Macro-Prep DEAE support, Bio-Rad) which had been preequilibrated in buffer BH + 0.2 M (NH4)2SO4. The flow-through (FT) from

(9) Cloning, expression and biochemical characterization of one Epsilon-class (GST-3) and ten Delta-class (GST-1) glutathione S-transferases from Drosophila melanogaster, and identification of additional nine members of the Epsilon classBiochemical JournalMarch 1, 2003RafaÅ‚ SAWICKI, Sharda P. SINGH, Ashis K. MONDAL, Helen BENEÅ , Piotr ZIMNIAK7.5), 50 mM KCl, centrifuged for 30 min at 30 000 g and the supernatant was passed over a DEAE column (Macro-Prep DEAE Support ; BioRad Laboratories), which did not retain DmGSTE1-1. The flowthrough was then applied to a Macro-Prep High Q Support

(10) The Maize Homologue of the Cell Cycle Checkpoint Protein MAD2 Reveals Kinetochore Substructure and Contrasting Mitotic and Meiotic Localization PatternsJ. Cell BiolMay 3, 1999Hong-Guo Yu, Michael G. Muszynski, R. Kelly Dawea nickel column (Invitrogen), and then purified to near-homogeneity by ion-exchange chromatography (Macro-Prep DEAE Support; BioRad). Polyclonal antibodies against the purified thioredoxin-MAD2 protein were produced in rabbits

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