Chromatography, including liquid and gas chromatography, is a staple tool in biological research. Preparative chromatography is used in the purification of proteins, antibodies, pharmaceuticals, bioactive compounds, and even cell populations. Meanwhile, analytical chromatography enables the measurement of target analytes and also the discovery of new compounds. Products in the scope of the chromatography application include packed (or empty) columns, chromatography media like resins or beads, filtration and concentration devices, kits, and accessories. The chromatography mode is the physical or chemical means of separation, such as via affinity binding, ionic interactions, hydrophobicity, or size exclusion. Use the provided filters to help navigate our extensive catalog of chromatography tools and reagents.
Reverse phase chromatography is a specific type of liquid chromatography that has a non-polar, or hydrophobic, stationary phase through which a polar, or hydrophilic, mobile phase is eluted. Nucleic acid analysis, separation and isolation of biomolecules, and therapeutic drug monitoring can be done using reverse phase chromatography. As the mobile phase moves through the column, the different components adsorb to it with varying affinities. They are separated and detected as they move out of the stationary phase. One of the reasons reverse phase chromatography is so useful is because the mobile phase can be made more or less hydrophilic, the pH can be changed, and the temperature can be altered to provide the optimum conditions for a wide range of samples to be analyzed.