Magnesium Sulfate (MgSO4) Solution from New England Biolabs

Product Specs
ItemMagnesium Sulfate (MgSO4) Solution
CompanyNew England Biolabs
Price $26.00 Inquire
Catalog NumberB1003S
Quantity6 ml
ApplicationsDNA Analysis
Temperature-20 C

Specifications/Features

Storage:
-20°C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Click-code-seq protocol for genome-wide single-nucleotide-resolution mapping of major types of DNA modificationsSTAR ProtocAugust 10, 2026Lucie Lefevre, Emilie A. Aghajani, Navnit K. Singh, Livia Maria Anna Pietrow, Nikolai J.L. Püllen, Vakil Takhaveev, Shana J. Sturlamix,NEB,Cat#N0447S β-Nicotinamide adenine dinucleotide (NAD+),NEB,Cat#B9007S Magnesium Sulfate (MgSO4) Solution,NEB,Cat#B1003S Tween 20,Sigma-Aldrich,Cat#P9416 Ethanol,Merck Millipore,Cat#1.100983.1011 "UltraPure 1M Tris-HCl,

(2) Programmable one-pot polymerase-mediated DNA synthesis via temperature controlNature CommunicationsJuly 2, 2026Jinho Kim, Jangho Choi, Woojin Kim, Namjin Cho, Yushin Jung, Seongjun Park, Eunjin Choi, Chaerim Lee, Youngeun Kim, Taehoon Ryu, et al.at 25 °C; New England Biolabs, Cat. No. B9004S), 1 µL of 100 mM MgSO₄ (New England Biolabs, Cat. No. B1003S), and nuclease-free water to a final volume of 6 µL. The mixture was heated to 95 °C for 3 min, and

(3) Development of a loop‐mediated isothermal amplification‐lateral flow dipstick ( LAMP ‐ LFD ) assay and on‐site rapid detection evaluation for Rodentibacter heylii and Rodentibacter pneumotropicusAnimal Models and Experimental MedicineMay 28, 2026Huiqiong Yan, Sisi Chen, Yuhan Gan, Jing Xing, Yufang Feng, Xin Wen, Jie Fang, Jiangtao Du, Shasang Zhou, Honggang Guo, et al.Biotech, #B500055‐0500), 1 μL of 8 U Bst 2.0 DNA polymerase (NEB, #M0537S), and 6 mmol/L MgSO4 (NEB, #B1003S). Final working concentrations of the primers and probe were 5 μmol/L for F3 and B3, 40 μmol/L for biotinylated

(4) A One-Pot CRISPR/Cas12a-Based Platform for Contamination-Free Nucleic Acid Amplification DetectionBiosensors (Basel)March 19, 2026Wei Tantai, Qinfeng Xu, Wenjuan Zhang, Yanni Li, Hao Liuthermolabile UDG (M0372S), and the LAMP reagents, including Bst 2.0 WarmStart DNA polymerase (M0538S), MgSO4 (B1003S), dNTPs (N0447S), and 10× LAMP amplification buffer (2 mM MgSO4, 10 mM (NH4)2SO4, 50 mM KCl, 20 mM Tris-HCl,

(5) Strain-level antigen variation facilitates immune evasion in Bacteroides thetaiotaomicronJ ImmunolMarch 18, 2026Robert W P Glowacki, Jessica M Till, Orion D Brock, Vladimir Makarov, Morgan J Engelhart, Philip P Ahernno. B9004S), 125 µM dNTPs (Thermo Fisher Scientific, Cat. no. 10297018), 2.5 mM MgSO4 (NEB, Cat. no. B1003S), 1X SYBR Green I (Lonza, Cat. no. 50513), 500 nM gene specific or 65 nM 16S rRNA primer specific to

(6) Multiplexed TrAEL-seq captures DNA replication dynamics in mammalian cellsNucleic Acids ResMarch 14, 2026Neesha Kara, Laura Biggins, Alex Whale, Kieron May, Vera Grinkevich, Paola Garran-Garcia, Jhanavi Srinivasan, Peter J Rugg-Gunn, Claudia Ribeiro de Almeida, Samantha J Walker, et al.reaction mix containing 5 µl isothermal amplification buffer (NEB, B0537S), 3 µl 100 mM MgSO4 (NEB, B1003S), 2 µl 10 mM dNTPs, and 1 µl Bst 2 WarmStart DNA polymerase (NEB, M0538S) was added to the sample and

(7) Enhanced SfaTnpB enables single-base-specific, one-pot nucleic acid detection for high-sensitivity diagnosticsNucleic Acids ResearchJanuary 8, 2026Bingrong Xu, Sheng Li, Yong Li, Shuhong Zhao, Xinyun Li, Jianlin Han, Di Wu, Shuaicheng Li, Ling Chen, Shengsong Xie, et al.No. N0447L, USA), Isothermal Amplification Buffer (NEB, Cat. No. B0374S, USA), MgSO4 (NEB, Cat. No. B1003S, USA), SfaTnpB nuclease, engineered ωRNA, 10× FastDigest Buffer, and an ssDNA-FQ reporter (5′-ROX/GTATCCAGTGCG/BHQ2-3′).

(8) Single cell transcriptional perturbome in pluripotent stem cell modelsMolecular Systems BiologyDecember 10, 2025Elisa Balmas, Maria L Ratto, Kirsten E Snijders, Silvia Becca, Carla Liaci, Irene Ricca, Giorgio R Merlo, Raffaele A Calogero, Luca Alessandrì, Sasha Mendjan, et al.Elution buffer,QIAGEN,#19086 Deoxynucleotide (dNTP) Solution Mix (10 mM),New England Biolabs,#N0447 MgSO4 (100 mM),New England Biolabs,#B1003S Isothermal Amplification Buffer II (10X),New England Biolabs,#B0374S Bst 3.0 DNA Polymerase (8 U/µL),New England Biolabs

(9) A specific, stable, and accessible LAMP assay targeting the HSP70 gene of Trypanosoma cruziMicrobiology SpectrumNovember 4, 2025Sneider Alexander Gutierrez Guarnizo, Beth Jessy Condori, Luciana Basma, Shirley Equilia, Edith Malaga, Siena Defazio, Emily Arteaga, Jean Karla Velarde, Martín Obregón, Anshule Takyar, et al.primer, 0.5 pmol LF primer, 0.5 pmol LB primer, 2.5 mM dNTPs (NEB, CN: N0447S), 0.8 mM MgSO₄ (NEB, CN: B1003S), 1 M Betaine (Sigma-Aldrich, CN: B0300-5VL), 1× Isothermal Amplification Buffer (20 mM Tris-HCl pH

(10) Click-code-seq reveals strand biases of DNA oxidation and depurination in human genomeNature Chemical BiologyOctober 31, 2025Vakil Takhaveev, Nikolai J. L. Püllen, Navnit K. Singh, Lucie Lefevre, Emilie A. Aghajani, Sabrina M. Huber, Stefan Schauer, Hailey L. Gahlon, Anna R. Poetsch, Shana J. Sturlaµl of 10× ThermoPol buffer (NEB, B9004S), 5 µl of dNTP mix (NEB, N0447S, 2 mM), 1 µl of MgSO4 (NEB, B1003S), 1 µl of ex_UMI (10 µM; Supplementary Table 1), 1 µl of Pr_P7 (10 µM; Supplementary Table 1) and 0.5

(11) An extraction-free and one-pot two-temperature CRISPR/Cas12b system for visual detection of Group B Streptococcus by integrating with RPAJournal of Clinical MicrobiologyOctober 8, 2025Chengman Zhao, Ge Li, Chen Shen, Yanjun Xie, Yun Chen, Xiaobo Ying, Yan Chen, Chuanling ZhangCat. No. 25102);1.0 µL dNTP mix (25 mM; Hongene, Cat. No. DN32);2.0 µL MgSO₄ (100 mM; NEB, Cat. No. B1003S);2.5 µL 10 × LAMP primer mix;6.0 µL glycine (2 M; Sangon Biotech, Cat. No. A610235);1.0 µL Bst DNA polymerase

(12) A rapid and accurate method for Helicobacter pylori detection via integrating LAMP assay with CRISPR/Cas12b detection by one-step in one-potFrontiers in Cellular and Infection MicrobiologyAugust 26, 2025Yeqian Zhang, Tao Liu, Puhua Zhang, Bo Ni, Xingang Wang, Long Bai, Wei Sun, Yujing Guan, Xiang Xia, Hui Cao, et al.(25102, Tolo Biotech, China), 1.0 μL dNTP mix (25 mM, DN32, Hongene, China), 1.5 μL MgSO4 (100 mM, B1003S, NEB, USA), 2.5 μL 10× HP-LAMP primer mix, 6 μL Glycine (2 M, A610235, Sangon, China), 1.25 μL HOLMES

(13) Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNANat CommunJuly 1, 2025Menglu Hu, Bingni Zhang, Yuanyue Shan, Feng Cao, Yihui Wang, Weiwei Qi, Xue Wang, Yuting Shen, Xinyi Guo, Mengmeng Zhang, et al.nM F3/B3 primers, 1.6 µM FIP/BIP primers, 400 nM Loop F/B primers, 6 mM MgSO4 (NEB, catalog number: B1003S), 1.4 mM dNTP mix (Sangon, catalog number: A610056), and 2 µL template in 1 \documentclass[12pt]{minimal}

(14) Oligonucleotide subsets selection by single nucleotide resolution barcode identificationNat CommunFebruary 12, 2025Woojin Kim, Mingweon Chon, Yoonhae Koh, Hansol Choi, Eunjin Choi, Hyewon Park, Yushin Jung, Taehoon Ryu, Sunghoon Kwon, Yeongjae Choi1 μL of Bst DNA Polymerase, 3 μL of 100 mM Magnesium Sulfate (MgSO4) (New England Biolabs, cat. no. B1003S) Solution, 5 μL of ThermoPol® Reaction Buffer, 1 μL of 10 mM dNTP, and 40 μL of nuclease-free water.

(15) A specific, stable, and accessible LAMP assay targeting the HSP70 gene of Trypanosoma cruzimedRxivJanuary 13, 2025Sneider Alexander Gutierrez Guarnizo, Luciana Basma, Shirley Equilia, Beth Jessy Condori, Edith Malaga, Siena Defazio, Emily Arteaga, Jean Karla Velarde, Martín Obregón, Anshule Takyar, et al.primer, 0.5 pmol LF primer, 0.5 pmol LB primer, 2.5 mM dNTPs (NEB, CN: N0447S), 0.8 mM MgSO₄ (NEB, CN: B1003S), 1 M Betaine (Sigma-Aldrich, CN: B0300–5VL), 1X Isothermal Amplification Buffer (20 mM Tris-HCl pH

(16) DEPICT-seq: Single-Cell Transcriptomic Analysis of Rare Cell Subsets Isolated via Nucleic Acid CytometryAnalytical ChemistryOctober 15, 2024Kaixuan Bao, Xiaochun Jiang, Hong-min Hu, Tiemin Liu, Jingwei ZhangμL droplet pellet was placed in 20 μL of DE-PCR outer buffer with an additional 2.4 mM MgSO4 (NEB, B1003S) to increase Taq activity. PCR reactions were performed as follows: 95 °C 5 min denature, followed by

(17) Comparison and Optimization of Simple DNA Extraction Methods for LAMP-Based Point-of-Care Applications Employing Submillimeter Skin BiopsiesACS OmegaSeptember 17, 2024Juan M. Boza, Jason Cade Manning, David C. Erickson2a–d). The LAMP assay composition (Table S3) includes 10× isothermal buffer (NEB B0537S), 100 mM MgSO4 (NEB B1003S), 10 mM dNTPs (NEB N0447L), and BST 2.0 WarmStart polymerase (NEB M0538S), which were purchased from

(18) An accurate and convenient method for Mycoplasma pneumoniae via one-step LAMP-CRISPR/Cas12b detection platformFrontiers in Cellular and Infection MicrobiologyAugust 8, 2024Tao Liu, Qing Liu, Fuqun Chen, Ying Shi, Guliya Maimaiti, Zhanhua Yang, Shutao Zheng, Xiaomei Lu, Hui Li, Zhaoyun ChenLAMP nuclear amplification kit (25102, TOLO Biotech, China) and dNTP mix (DN32, Hongene, China) MgSO4 (B1003S, NEB, USA), Glycine (A610235, Sangon, China), SYTO-9 (S34854ThermoFisher, USA), AapCas12 (32118, TOLO

(19) Thorough molecular configuration analysis of non-canonical AAV genomes in AAV vector preparationsMolecular Therapy - Methods & Clinical DevelopmentMarch 14, 2024Junping Zhang, Xiangping Yu, Matthew Chrzanowski, Jiahe Tian, Derek Pouchnik, Ping Guo, Roland W. Herzog, Weidong XiaoBuffer (New England Biolabs, catalog no. B9004S)100 mM MgSO4 solution (New England Biolabs, catalog no. B1003S)dNTP Set 100 mM Solutions (Thermo Scientific, catalog no. R0181)Bst 2.0 polymerase (New England Biolabs,

(20) Evaluation of molecular inhibitors of loop-mediated isothermal amplification (LAMP)Sci RepMarch 11, 2024May Khat Nwe, Nisachon Jangpromma, Lapatrada TaemaitreeCYTB-LF and CYTB-LB (10 µM, 0.5 µL), dNTPs (10 mM, 1.5 µL, NEB, N0447S), MgSO4 (100 mM, 0.5 µL, NEB, B1003S), reaction buffer ([200 mM Tris-HCl, 100 mM (NH4)2SO4, 500 mM KCl, 20 mM MgSO4, 1% Tween® 20, pH 8.8

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