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Protein Biochemistry
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Ponceau S solution
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Ponceau S solution
Ponceau S solution from Santa Cruz Biotechnology, Inc.
(1)
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Citations:
(21)
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Supplier Page from
Santa Cruz Biotechnology, Inc. for
Ponceau S solution
Description
Ponceau S solution
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Ponceau S solution
Company
Santa Cruz Biotechnology, Inc.
Price
$81.00
Supplier Page
View Company Product Page
Catalog Number
sc-301558
Quantity
1 L
Type
Cell Stains and Indicators
Molecular Weight
760.57
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Santa Cruz Biotechnology, Inc.
10410 Finnell Street
Dallas
,
Texas
75220
United States
Phone
: 1-800-457-3801
831-457-3801
Company Profile
Website:
http://www.scbt.com/
Citations (21)
(1)
The Zn2+ transporter ZIP7 enhances endoplasmic-reticulum-associated protein degradation and prevents neurodegeneration in Drosophila
Developmental Cell
July 1, 2024
Xiaoran Guo, Morgan Mutch, Alba Yurani Torres, Maddalena Nano, Nishi Rauth, Jacob Harwood, Drew McDonald, Zijing Chen, Craig Montell, Wei Dai,
et al.
Scientific, #A412P-4). Transfer quality was assessed by Ponceau
staining
(
Santa Cruz Biotechnology
, #
sc-301558
).
Membranes
were blocked 30 min at RT in agitation in Intercept® (TBS) Blocking Buffer (
LI-COR Biosciences
,
(2)
Proteasome assembly chaperone translation upon stress requires Ede1 phase separation at the plasma membrane
iScience
January 19, 2024
Thomas D. Williams, Aurellia Winaya, Ifeoluwapo Joshua, Adrien Rousseau
30 min on the BioRad TurboBlot system.
Membranes
were stained with
ponceau S solution
(
Santa Cruz
,
sc-301558
), imaged, then soaked in TBS-T (TBS +0.1% Tween 20) containing 5% milk powder for at least 1 hour, before
(3)
The ribosome‐associated chaperone Zuo1 controls translation upon TORC1 inhibition
The EMBO Journal
December 11, 2023
Ailsa Black, Thomas D Williams, Flavie Soubigou, Ifeoluwapo M Joshua, Houjiang Zhou, Frederic Lamoliatte, Adrien Rousseau
,
Thermo Fisher
,Cat #
A25776
GFP
binder beads,MRC PPU Reagents and Services,DU54283 Ponceau S,
Santa Cruz
,Cat #
sc‐301558
Clarity Western ECL substrate,Bio‐Rad,Cat #170‐5061 Clarity Max Western ECL substrate,Bio‐Rad,Cat
(4)
AGAP1-associated endolysosomal trafficking abnormalities link gene-environment interactions in neurodevelopmental disorders
Disease models & mechanisms
September 1, 2023
Lewis SA, Bakhtiari S, Forstrom J, Bayat A, Bilan F, Le Guyader G, Alkhunaizi E, Vernon H, Padilla-Lopez SR, Kruer MC.
rabbit anti-Ref(2)P (
1:500
; Abcam, 178440) normalized using Ponceau
staining
(
Santa Cruz Biotechnology
,
sc-301558
), or
mouse
anti-β-actin (
1:2000
;
Abcam
, 8224) and rabbit
anti-Atg8
(
1:2000
;
Sigma-Aldrich
,
ABC974
).
(5)
Multiple phosphorylation of the Cdc48/p97 cofactor protein Shp1/p47 occurs upon cell stress in budding yeast
Life science alliance
April 1, 2023
Agrotis A, Lamoliatte F, Williams TD, Black A, Horberry R, Rousseau A.
using semidry transfer apparatus (
1704150
;
Bio-Rad
).
Membranes
were stained with
Ponceau S solution
(
sc-301558
;
Santa Cruz
) before imaging on a
Bio-Rad
ChemiDoc MP
System to confirm equal protein loading.
Membranes
(6)
AGAP1-associated endolysosomal trafficking abnormalities link gene-environment interactions in a neurodevelopmental disorder
bioRxiv
February 4, 2023
Sara A. Lewis, Somayeh Bakhtiari, Jacob Forstrom, Allan Bayat, Frédéric Bilan, Gwenaël Le Guyader, Ebba Alkhunaizi, Hilary Vernon, Sergio R. Padilla-Lopez, Michael C. Kruer
autophagy:
1:500
rabbit α Ref(2)P (Abcam 178440) normalized using Ponceau
staining
(
Santa Cruz Biotechnology
sc-301558
) or
1:2000
mouse
α beta actin (Abcam 8224) and
1:2000
rabbit α Atg8 (
Sigma
ABC974
). Antibodies used
(7)
Actin remodelling controls proteasome homeostasis upon stress
Nature cell biology
July 1, 2022
Williams TD, Cacioppo R, Agrotis A, Black A, Zhou H, Rousseau A.
TransBlot Turbo
(
Bio-Rad
) (30 min, 2.5 Amp).
Membranes
were stained with
Ponceau S solution
(
Santa Cruz
,
sc-301558
), imaged, cut, washed in TBS and blotted for at least 1 h with TBS containing 5% milk, washed three
(8)
Development of a novel model of intervertebral disc degeneration by the intradiscal application of monosodium iodoacetate (MIA) in rat
The spine journal : official journal of the North American Spine Society
January 1, 2022
Suh HR, Cho HY, Han HC.
(9)
Nedd9 Restrains Autophagy to Limit Growth of Early Stage Non-Small Cell Lung Cancer
Cancer research
July 1, 2021
Deneka AY, Kopp MC, Nikonova AS, Gaponova AV, Kiseleva AA, Hensley HH, Flieder DB, Serebriiskii IG, Golemis EA.
(10)
Toll-like receptor 4 (TLR4) signaling in the trigeminal ganglion mediates facial mechanical and thermal hyperalgesia in rats
Physiology & behavior
November 1, 2020
Araya EI, Barroso AR, Turnes JM, Radulski DR, Jaganaught JA, Zampronio AR, Chichorro JG.
(11)
Fruticuline A, a chemically-defined diterpene, exerts antineoplastic effects in vitro and in vivo by multiple mechanisms
Scientific reports
October 5, 2020
Corso CR, Stipp MC, Radulski DR, Mariott M, da Silva LM, de Souza Ramos EA, Klassen G, Queiroz Telles JE, Oliveira CS, Stefanello MÉA,
et al
.
[pH 7.6], 192 mM glicine, 20% methanol and 0.02% SDS). The
membranes
were stained using Ponceau S (
sc-301558
,
Santa Cruz Biotechnologies
, Dallas, TX, USA) and then cut. Nonspecific bindings were blocked using
(12)
Ciliary Neurotrophic Factor Acts on Distinctive Hypothalamic Arcuate Neurons and Promotes Leptin Entry Into and Action on the Mouse Hypothalamus
Frontiers in cellular neuroscience
January 1, 2020
Venema W, Severi I, Perugini J, Di Mercurio E, Mainardi M, Maffei M, Cinti S, Giordano A.
(13)
Effects of varying extracellular amino acid profile on intracellular free amino acid concentrations and cell signaling in primary mammary epithelial cells
Journal of dairy science
October 1, 2019
Yoder PS, Ruiz-Cortes T, Castro JJ, Hanigan MD.
(14)
Leptin signaling impairs macrophage defenses against
Salmonella
Typhimurium
Proceedings of the National Academy of Sciences of the United States of America
August 13, 2019
Fischer J, Gutièrrez S, Ganesan R, Calabrese C, Ranjan R, Cildir G, Hos NJ, Rybniker J, Wolke M, Fries JWU,
et al
.
was used as a loading control. For
phagosome
samples, Ponceau
staining
was used as loading control (
sc-301558
,
Santa Cruz Biotechnology
). After
incubation
with secondary
horseradish
peroxidase-conjugated antibodies
(15)
Ponceau S waste: Ponceau S staining for total protein normalization
Analytical biochemistry
June 15, 2019
Sander H, Wallace S, Plouse R, Tiwari S, Gomes AV.
designed for rapid (5 min)
staining
on nitrocellulose or PVDF
membranes
[26].
Santa Cruz Biotechnology
,
sc-301558
,0.1 % (w/v) in 5% (v/v) acetic acid,Rapid (5 min)
staining
of protein bands on PVDF or nitrocellulose
(16)
Biological Effects of Ciliary Neurotrophic Factor on hMADS Adipocytes
Frontiers in endocrinology
January 1, 2019
Perugini J, Di Mercurio E, Tossetta G, Severi I, Monaco F, Reguzzoni M, Tomasetti M, Dani C, Cinti S, Giordano A.
(17)
Solubility changes of promyelocytic leukemia (PML) and SUMO monomers and dynamics of PML nuclear body proteins in arsenite-treated cells
Toxicology and applied pharmacology
December 1, 2018
Hirano S, Udagawa O, Kobayashi Y, Kato A.
(18)
Salt-losing nephropathy in mice with a null mutation of the Clcnk2 gene
Acta physiologica (Oxford, England)
November 1, 2016
Grill A, Schießl IM, Gess B, Fremter K, Hammer A, Castrop H.
(19)
Skeletal Muscle Regeneration and Oxidative Stress Are Altered in Chronic Kidney Disease
PloS one
January 1, 2016
Avin KG, Chen NX, Organ JM, Zarse C, O'Neill K, Conway RG, Konrad RJ, Bacallao RL, Allen MR, Moe SM.
(20)
Selective, rapid and optically switchable regulation of protein function in live mammalian cells
Nature chemistry
July 1, 2015
Tsai YH, Essig S, James JR, Lang K, Chin JW.
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11/11/2015
Ponceau S Solution
M M
Solution is used to detect protein bands on PVDF membranes to determine protein transfer efficiency in western blots. Easy to use solution that can be destained with NaOH.
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Ponceau S solution from Santa Cruz Biotechnology, Inc.
Supplier Page
View Company Product Page
$81.00
Product Specs
Company Info
Citations
Reviews
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