Ponceau S solution from Santa Cruz Biotechnology, Inc.

Product Specs
ItemPonceau S solution
CompanySanta Cruz Biotechnology, Inc.
Price $81.00Supplier Page View Company Product Page
Catalog Numbersc-301558
Quantity1 L
TypeCell Stains and Indicators
Molecular Weight760.57

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) The Zn2+ transporter ZIP7 enhances endoplasmic-reticulum-associated protein degradation and prevents neurodegeneration in DrosophilaDevelopmental CellJuly 1, 2024Xiaoran Guo, Morgan Mutch, Alba Yurani Torres, Maddalena Nano, Nishi Rauth, Jacob Harwood, Drew McDonald, Zijing Chen, Craig Montell, Wei Dai, et al.Scientific, #A412P-4). Transfer quality was assessed by Ponceau staining (Santa Cruz Biotechnology, #sc-301558). Membranes were blocked 30 min at RT in agitation in Intercept® (TBS) Blocking Buffer (LI-COR Biosciences,

(2) Proteasome assembly chaperone translation upon stress requires Ede1 phase separation at the plasma membraneiScienceJanuary 19, 2024Thomas D. Williams, Aurellia Winaya, Ifeoluwapo Joshua, Adrien Rousseau30 min on the BioRad TurboBlot system. Membranes were stained with ponceau S solution (Santa Cruz, sc-301558), imaged, then soaked in TBS-T (TBS +0.1% Tween 20) containing 5% milk powder for at least 1 hour, before

(3) The ribosome‐associated chaperone Zuo1 controls translation upon TORC1 inhibitionThe EMBO JournalDecember 11, 2023Ailsa Black, Thomas D Williams, Flavie Soubigou, Ifeoluwapo M Joshua, Houjiang Zhou, Frederic Lamoliatte, Adrien Rousseau,Thermo Fisher,Cat #A25776 GFP binder beads,MRC PPU Reagents and Services,DU54283 Ponceau S,Santa Cruz,Cat #sc‐301558 Clarity Western ECL substrate,Bio‐Rad,Cat #170‐5061 Clarity Max Western ECL substrate,Bio‐Rad,Cat

(4) AGAP1-associated endolysosomal trafficking abnormalities link gene-environment interactions in neurodevelopmental disordersDisease models & mechanismsSeptember 1, 2023Lewis SA, Bakhtiari S, Forstrom J, Bayat A, Bilan F, Le Guyader G, Alkhunaizi E, Vernon H, Padilla-Lopez SR, Kruer MC.rabbit anti-Ref(2)P (1:500; Abcam, 178440) normalized using Ponceau staining (Santa Cruz Biotechnology, sc-301558), or mouse anti-β-actin (1:2000; Abcam, 8224) and rabbit anti-Atg8 (1:2000; Sigma-Aldrich, ABC974).

(5) Multiple phosphorylation of the Cdc48/p97 cofactor protein Shp1/p47 occurs upon cell stress in budding yeastLife science allianceApril 1, 2023Agrotis A, Lamoliatte F, Williams TD, Black A, Horberry R, Rousseau A.using semidry transfer apparatus (1704150; Bio-Rad). Membranes were stained with Ponceau S solution (sc-301558; Santa Cruz) before imaging on a Bio-Rad ChemiDoc MP System to confirm equal protein loading. Membranes

(6) AGAP1-associated endolysosomal trafficking abnormalities link gene-environment interactions in a neurodevelopmental disorderbioRxivFebruary 4, 2023Sara A. Lewis, Somayeh Bakhtiari, Jacob Forstrom, Allan Bayat, Frédéric Bilan, Gwenaël Le Guyader, Ebba Alkhunaizi, Hilary Vernon, Sergio R. Padilla-Lopez, Michael C. Kruerautophagy: 1:500 rabbit α Ref(2)P (Abcam 178440) normalized using Ponceau staining (Santa Cruz Biotechnology sc-301558) or 1:2000 mouse α beta actin (Abcam 8224) and 1:2000 rabbit α Atg8 (Sigma ABC974). Antibodies used

(7) Actin remodelling controls proteasome homeostasis upon stressNature cell biologyJuly 1, 2022Williams TD, Cacioppo R, Agrotis A, Black A, Zhou H, Rousseau A.TransBlot Turbo (Bio-Rad) (30 min, 2.5 Amp). Membranes were stained with Ponceau S solution (Santa Cruz, sc-301558), imaged, cut, washed in TBS and blotted for at least 1 h with TBS containing 5% milk, washed three

(8) Development of a novel model of intervertebral disc degeneration by the intradiscal application of monosodium iodoacetate (MIA) in ratThe spine journal : official journal of the North American Spine SocietyJanuary 1, 2022Suh HR, Cho HY, Han HC.

(9) Nedd9 Restrains Autophagy to Limit Growth of Early Stage Non-Small Cell Lung CancerCancer researchJuly 1, 2021Deneka AY, Kopp MC, Nikonova AS, Gaponova AV, Kiseleva AA, Hensley HH, Flieder DB, Serebriiskii IG, Golemis EA.

(10) Toll-like receptor 4 (TLR4) signaling in the trigeminal ganglion mediates facial mechanical and thermal hyperalgesia in ratsPhysiology & behaviorNovember 1, 2020Araya EI, Barroso AR, Turnes JM, Radulski DR, Jaganaught JA, Zampronio AR, Chichorro JG.

(11) Fruticuline A, a chemically-defined diterpene, exerts antineoplastic effects in vitro and in vivo by multiple mechanismsScientific reportsOctober 5, 2020Corso CR, Stipp MC, Radulski DR, Mariott M, da Silva LM, de Souza Ramos EA, Klassen G, Queiroz Telles JE, Oliveira CS, Stefanello MÉA, et al.[pH 7.6], 192 mM glicine, 20% methanol and 0.02% SDS). The membranes were stained using Ponceau S (sc-301558, Santa Cruz Biotechnologies, Dallas, TX, USA) and then cut. Nonspecific bindings were blocked using

(12) Ciliary Neurotrophic Factor Acts on Distinctive Hypothalamic Arcuate Neurons and Promotes Leptin Entry Into and Action on the Mouse HypothalamusFrontiers in cellular neuroscienceJanuary 1, 2020Venema W, Severi I, Perugini J, Di Mercurio E, Mainardi M, Maffei M, Cinti S, Giordano A.

(13) Effects of varying extracellular amino acid profile on intracellular free amino acid concentrations and cell signaling in primary mammary epithelial cellsJournal of dairy scienceOctober 1, 2019Yoder PS, Ruiz-Cortes T, Castro JJ, Hanigan MD.

(14) Leptin signaling impairs macrophage defenses against Salmonella TyphimuriumProceedings of the National Academy of Sciences of the United States of AmericaAugust 13, 2019Fischer J, Gutièrrez S, Ganesan R, Calabrese C, Ranjan R, Cildir G, Hos NJ, Rybniker J, Wolke M, Fries JWU, et al.was used as a loading control. For phagosome samples, Ponceau staining was used as loading control (sc-301558, Santa Cruz Biotechnology). After incubation with secondary horseradish peroxidase-conjugated antibodies

(15) Ponceau S waste: Ponceau S staining for total protein normalizationAnalytical biochemistryJune 15, 2019Sander H, Wallace S, Plouse R, Tiwari S, Gomes AV.designed for rapid (5 min) staining on nitrocellulose or PVDF membranes [26]. Santa Cruz Biotechnology,sc-301558,0.1 % (w/v) in 5% (v/v) acetic acid,Rapid (5 min) staining of protein bands on PVDF or nitrocellulose

(16) Biological Effects of Ciliary Neurotrophic Factor on hMADS AdipocytesFrontiers in endocrinologyJanuary 1, 2019Perugini J, Di Mercurio E, Tossetta G, Severi I, Monaco F, Reguzzoni M, Tomasetti M, Dani C, Cinti S, Giordano A.

(17) Solubility changes of promyelocytic leukemia (PML) and SUMO monomers and dynamics of PML nuclear body proteins in arsenite-treated cellsToxicology and applied pharmacologyDecember 1, 2018Hirano S, Udagawa O, Kobayashi Y, Kato A.

(18) Salt-losing nephropathy in mice with a null mutation of the Clcnk2 geneActa physiologica (Oxford, England)November 1, 2016Grill A, Schießl IM, Gess B, Fremter K, Hammer A, Castrop H.

(19) Skeletal Muscle Regeneration and Oxidative Stress Are Altered in Chronic Kidney DiseasePloS oneJanuary 1, 2016Avin KG, Chen NX, Organ JM, Zarse C, O'Neill K, Conway RG, Konrad RJ, Bacallao RL, Allen MR, Moe SM.

(20) Selective, rapid and optically switchable regulation of protein function in live mammalian cellsNature chemistryJuly 1, 2015Tsai YH, Essig S, James JR, Lang K, Chin JW.

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11/11/2015Ponceau S Solution M MSolution is used to detect protein bands on PVDF membranes to determine protein transfer efficiency in western blots. Easy to use solution that can be destained with NaOH.