Fluo-3 AM, Fluorescent calcium Indicator from Abcam

Product Specs
ItemFluo-3 AM, Fluorescent calcium Indicator
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab145254
Quantity1 mg
TypeChemical
Molecule NameFluo-3 AM, Fluorescent calcium Indicator

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Silymarin Modulates 3-Nitropropionic Acid-Induced Oxidative Neurotoxicity and Apoptosis Through the Inhibition of TRPV1 Channel in Mouse Brain and Hippocampal NeuronsJ Biochem Mol ToxicolJuly 17, 2026Abdulsamed Kükürt, Ömer Faruk Başerhippocampal cells cultured in 35 mm glass‐bottom dishes were incubated with 1 µM Fluo‐3/AM dye (Cat # ab145254, Abcam Inc., Cambridge, UK) for 60–90 min at 37°C in the dark. Following incubation, cells were washed

(2) Boric Acid Diminishes Sciatic Nerve Injury-Induced Apoptosis, Oxidative Stress, and Pain via The Block of TRPV1 Channel in MiceBiological Trace Element ResearchJune 28, 2025Kemal Ertilav, Mustafa Nazıroğluat 488 nm, the whole sciatic nerve samples were dyed for 60—90 min using 1 µM Fluo −3/AM dye (Cat # ab145254, Abcam Inc., Cambridge, UK) [15]. Changes in fluorescence intensity in collected green images were measured

(3) Multifunctional piezoelectric hydrogels under ultrasound stimulation boost chondrogenesis by recruiting autologous stem cells and activating the Ca2+/CaM/CaN signaling pathwayBioact MaterApril 18, 2025Yu-Bao Liu, Xu Liu, Xiao-Fei Li, Liang Qiao, Hao-Liang Wang, Yue-Fu Dong, Feng Zhang, Yang Liu, Hao-Yang Liu, Ming-Liang Ji, et al.a single-cell suspension was prepared. The cells were stained with the calcium indicator Fluo-3AM (ab145254, Abcam), incubated, washed, and then subjected to machine detection (LSRFortessa X-20, BD Biosciences). 4.20

(4) Resveratrol Modulates Diabetes-Induced Neuropathic Pain, Apoptosis, and Oxidative Neurotoxicity in Mice Through TRPV4 Channel InhibitionMolecular NeurobiologySeptember 1, 2024Haci Ömer Osmanlıoğlu, Mustafa Nazıroğlustimulated by an argon laser at 488 nm, the DRGs were stained for an hour using 1 µM Fluo-3/AM dye (Cat # ab145254, Abcam Inc., Cambridge, UK) [6]. Using a special program (ZEN, blue edition), differences in the intensity

(5) Mitochondrial dysfunction induced by HIF-1α under hypoxia contributes to the development of gastric mucosal lesionsClinical and translational medicineApril 1, 2024Xiao Y, Liu X, Xie K, Luo J, Zhang Y, Huang X, Luo J, Tan S.intracellular Ca2+ concentration ([Ca2+]i) was measured by Fluo‐3AM (a fluorescent Ca2+ indicator, ab145254, Abcam) and the mitochondrial Ca2+ concentration ([Ca2+]m) was examined by Rhod‐2AM (a fluorescent Ca2+

(6) Soluble (pro)renin receptor induces endothelial dysfunction and hypertension in mice with diet-induced obesity via activation of angiotensin II type 1 receptorClinical science (London, England : 1979)March 26, 2021Fu Z, Wang F, Liu X, Hu J, Su J, Lu X, Lu A, Cho JM, Symons JD, Zou CJ, et al.calcium concentration (Ca2+) The intracellular Ca2+ concentration was measured using Fluo-3-AM (Abcam, ab145254). HUVECs were washed three times with PBS and incubated with 10μM Fluo-3-AM for 30 min at 37 °C in 5%

(7) Protective Effect of Epigallocatechin-3-Gallate in Hydrogen Peroxide-Induced Oxidative Damage in Chicken LymphocytesOxidative Medicine and Cellular LongevityDecember 31, 2020Xiaoqing Chi, Xiaodan Ma, Zoushuyi Li, Yong Zhang, Yong Wang, Lijia Yuan, Ye Wu, Wei Xu, Songhua Hugenomic DNA extraction kit (ab156900), and intracellular calcium ion ([Ca2+]i) detection kit (Fluo3/AM) (ab145254) were the products of Abcam (Cambridge, UK); apoptosis detection kit (556547) was the product of BD Biosciences

(8) Obesity-induced overexpression of miR-802 impairs insulin transcription and secretionNature CommunicationsDecember 1, 2020Fangfang Zhang et al.anti-miR-802 (len-anti-miR-802) for 48 h. The intracellular calcium contents were measured by Fluo-3M (Abcam, ab145254), according to the manufacturer’s instructions, using flow cytometry and immunofluorescence. RNA-sequencing

(9) Calcium‑sensing receptor promotes high glucose‑induced myocardial fibrosis via upregulation of the TGF‑β1/Smads pathway in cardiac fibroblastsMolecular medicine reportsAugust 1, 2019Yuan H, Fan Y, Wang Y, Gao T, Shao Y, Zhao B, Li H, Xu C, Wei C.treated with HG (40 mM), R568 (5 µM) or Calhex231 (3 µM) were stained using 5 mM Fluo-3/AM (cat. no. ab145254; Abcam) for 30 min at 37°C in the dark. Subsequently, the cells were washed with Ca2+-free Tyrode's

(10) Baicalein inhibits fibronectin-induced epithelial-mesenchymal transition by decreasing activation and upregulation of calpain-2Cell death & diseaseApril 18, 2019Chen Y, Chen L, Hong D, Chen Z, Zhang J, Fu L, Pan D, Zhang Y, Xu Y, Gan S, et al.flow cytometric estimation of Fluo-3 AM. The cells were collected and loaded with 3 μM Fluo-3 AM (Abcam, ab145254) for 1 h at 37 °C in the dark, and then resuspended with 500 μl phosphate-buffered saline. The fluorescence

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Fluo-3 AM, Fluorescent calcium Indicator from Abcam

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Fluo-3 AM, Fluorescent calcium Indicator from Abcam

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