Rhod-2 AM, fluorescent Ca2+ indicator from Abcam

Product Specs
ItemRhod-2 AM, fluorescent Ca2+ indicator
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab142780
Quantity1 mg
Purity0.93
TypeChemical
Molecule NameRhod-2 AM

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(1) ChREBP-β Exacerbates Renal Tubular Disorders Caused by Fructose via ATF4FASEB JApril 30, 2026Ting Fang, Xinyu Yang, Xiaoqing Deng, Jingyi Wang, Ting Li, Hang Guo, Bei Sun, Liming ChenMeasurements Human kidney‐2 (HK2) cells were treated with 2 μM Fura‐2 AM (ab120873, Abcam) and 1 μM Rhod2‐AM (ab142780, Abcam) in a Pluronic‐F27 solution for 15 min at 37°C, followed by a wash. Time‐lapse fluorescence imaging

(2) JP2 and JCN Crosstalk Abrogate MURF1-Mediated JCN Ubiquitination and Degradation in CardiomyocytesJACC Basic Transl SciApril 17, 2026Xiaoyun Ji, Yifan Huang, Jinxi Wang, Zhifeng Dong, Rui Ni, Zijun Su, Dong Zheng, Guo-Chang Fan, Douglas L. Jones, Long-Sheng Song, et al.Methods.45 Briefly, after treatments, cardiomyocytes were stained with Rhod-2 AM (2 μmol/L, Abcam, ab142780) for 30 minutes at room temperature, washed twice and transferred to a bath chamber. The cardiomyocytes

(3) Clinically Relevant Doses of Remimazolam Modulate Cardiac Electrophysiology: Late Repolarization Prolongation and Increased Conduction Dispersion With Preserved QTcCardiol Res PractApril 13, 2026Zijun Wang, Ying Cao, Gao Su, Yanyan Feng, Rongfeng Yang, Xue Bai, Hong Gaoloading. For dual‐parameter optical mapping, RH237 (Chem Cruz, sc‐499456; 1 μg/mL) and Rhod‐2 AM (Abcam, ab142780; 1 μg/mL) were introduced sequentially. Illumination was provided by 530 nm light‐emitting diode (LED)

(4) Pim1 Serves as a Therapeutic Target for Inflammatory Arthritis via Mitochondrial Metabolism and Th17 Cell DifferentiationResearch (Wash D C)February 27, 2026Zepeng Su, Yipeng Zeng, Qibo Li, Jinteng Li, Guan Zheng, Weihao Zhang, Zipeng Xiao, Zibin Chen, Yangfeng Lin, Ziqian Liu, et al.#C2001S) for detection of the mitochondrial membrane potential, stained with 5 μM Rhod-2AM (Abcam, #ab142780) for detection of mito-Ca2+, and stained with 10 μM 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA;

(5) MiR-499-5P/PACS2/TRPV1 Axis Maintains Mitochondrial Homeostasis and Left Ventricular Function after Extreme Cold StressJ Cardiovasc Transl ResFebruary 5, 2026Renzheng Chen, Yan Ma, Sijia Chen, Cheng Qin, Hui Li, Naiyuan Sun, Feng Caoseeded on glass-bottomed cell culture dishes and incubated with 1μM of the calcium indicator Rhod2-AM (ab142780, Abcam) at 37 °C in the dark for 30 min. Next, cells were washed twice with Hanks’ Balanced Salt Solution

(6) Super mitochondria-enriched extracellular vesicles enable enhanced mitochondria transferNat CommunOctober 27, 2025Yi Wang, Hao-Yuan Yu, Zi-Juan Yi, Lian-Yu Qi, Jing-Song Yang, Hai-Xin Xie, Min Zhao, Na-Hui Liu, Jia-Qi Chen, Tian-Jiao Zhou, et al.HuaBio (Hangzhou, China). Anti-Vimentin mAb (ab92547), Anti-Complex I mAb (ab109798) and Rhod-2 AM (ab142780) were purchased from Abcam (Cambridge, UK). The siRNA targeting IP3R (siIP3R) from Riobio Company Limited

(7) Abnormal Ca2+ handling and reduced Ito contribute to citalopram-induced QT prolongation and cardiac arrhythmiasFrontiers in PharmacologySeptember 4, 2025Yangpeng Li, Jiamin Xie, Yuqing Zheng, Jianhong Li, Min Zhao, Li Liu, Hongping Shen, Ming Lei, Xiaoqiu Tan, Juan Xiao, et al.Then, 15 µL stock solution of Rhod2-AM (final concentration 0.356 mM, 1 mg/mL, stock solution, Cat: ab142780, Abcam, Cambridge, United Kingdom) and 15 µL Pluronic F127 (20% w/v in DMSO, Cat: P3000MP, Invitrogen,

(8) Targeting CB1R Rewires Ca2+-Dependent Mitophagy to Promote Nerve RegenerationTheranosticsAugust 11, 2025Ningning Wang, Weizhen Li, Tuo Yang, Baolong Li, Chuikai Meng, Xiongyao Zhou, Jialu Sun, Kaiming Yu, Shusen Cui, Rangjuan Caoactivity. Calcium imaging using Rhod-2 AM Mitochondrial Ca2+ levels were measured using Rhod-2 AM (Abcam, ab142780). Cells were incubated with 5 μM Rhod-2 AM in glass-bottom culture dishes at 37 °C for 30 min in the

(9) Filamented Light (FLight) Bioprinting of Mini‐Muscles with Self‐Renewal PotentialAdvanced MaterialsJuly 23, 2025Hao Liu, Michael Winkelbauer, Jakub Janiak, Patrick Weber, Ali Kerem Kalkan, Inseon Kim, Parth Chansoria, Ori Bar‐Nur, Marcy Zenobi‐Wongfluorescent Ca2+ indicator). Briefly, 10 µL of Rhod‐2 AM calcium dye (Stock solution: 1 mg mL−1, Abcam, AB142780) in dimethyl sulfoxide (DMSO) was carefully mixed with 10 µL of Pluronic F‐127 (diluted in DMSO as 20%

(10) Oleanolic acid enhanced the anticancer effect of fluorouracil by regulating Ca2+ levels in hepatocellular carcinoma cellsBraz J Med Biol ResApril 14, 2025Jing Dong, Yin Gao, Penghui Li, Ping Chen, Yanxin Lv, Yanan Liu, Song Zhang, Minglong Zhang, Yu Wangindicator (Thermo Fisher Scientific, USA, M14206); Rhod-2AM calcium ion fluorescent probe (Abcam, UK, ab142780); D-Hanks’ solution (PYG0079, Wuhan Doctoral Biotechnology Co., Ltd., China); MTT Cell Proliferation

(11) IL-1β enhances susceptibility to atrial fibrillation in mice by acting through resident macrophages and promoting caspase-1 expressionNature Cardiovascular ResearchFebruary 6, 2025Oscar Moreno-Loaiza, Vinicius Cardoso Soares, Manuela de Assumpção Souza, Narendra Vera-Nuñez, Ainhoa Rodriguez Rodriguez de Yurre Guirao, Tatiana Pereira da Silva, Ana Beatriz Pozes, Larissa Perticarrari, Evelin Monteiro, Maria Clara Albino, et al.saturated with O2. After approximately 5 min of stabilization, a fluorophore dye (Rhod-2- AM – ABCAM – #ab142780) diluted in oxygenated Tyrode’s solution was perfused. After dye-loading, Blebbistatin (4 μM) (Selleckchem,

(12) Dual calcium-voltage optical mapping of regional voltage and calcium signals in intact murine RyR2-R2474S heartsJournal of Molecular and Cellular Cardiology PlusDecember 1, 2024Yangpeng Li, Zhu Liu, Christopher O'Shea, Jianhong Li, Xian Luo, Tangting Chen, Xianhong Ou, Weichao Liu, Guoliang Hao, Christopher L.-H. Huang, et al.stock solution Cat: S1109, Thermo Fisher Scientific, USA) and Rhod2-AM (1 mg/mL, stock solution, Cat: ab142780, Abcam, UK) were perfused to enable simultaneous membrane voltage (Vm) and intracellular Ca2+ measurements

(13) Brain-to-heart cholinergic synapse-calcium signaling mediates ischemic stroke-induced atrial fibrillationTheranosticsOctober 7, 2024Yingran Liang, Gongxin Wang, Siwen Fan, Junyi Zhang, Shuang He, Guixiang Pan, Guoliang Hao, Yan ZhuLemeitian Pharmaceutical Technology Co., Ltd (Chengdu, China). Blebbistatin (ab120425) and Rhod-2AM (ab142780) were obtained from Abcam Plc (Cambridge, United Kingdom). F127 (P3000MP) was purchased from Invitrogen

(14) Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventionsNature communicationsSeptember 12, 2024Yang P, Zhu L, Wang S, Gong J, Selvaraj JN, Ye L, Chen H, Zhang Y, Wang G, Song W, et al.eliminate motion artifacts. Intracellular calcium changes were measured using 1 mg/mL RHOD-2AM (Abcam, ab142780). The entire heart was illuminated with 549 nm excitation light (MappingLab, LEDC-2001), and CMOS cameras

(15) CKB Promotes Mitochondrial ATP Production by Suppressing Permeability Transition PoreAdvanced ScienceAugust 1, 2024Le He, Jianghua Lin, Shaojuan Lu, Hao Li, Jie Chen, Xinyi Wu, Qixin Yan, Hailiang Liu, Hui Li, Yufeng Shimitochondrial Ca2+ concentrations were made using cells incubated with 5 µm Rhod‐2 Am probe (Abcam, ab142780) for 30 min to detect mCa2+, followed by incubation of 200 nm Mito‐tracker Green for another 20 min

(16) DDAH-1 maintains endoplasmic reticulum-mitochondria contacts and protects dopaminergic neurons in Parkinson’s diseaseCell Death & DiseaseJune 7, 2024Yichen Zhao, Weiwei Shen, Minjie Zhang, Min Guo, Yunxiao Dou, Sida Han, Jintai Yu, Mei Cui, Yanxin Zhaocalcium imaging Cells were loaded with the fluorescent mitochondrial Ca2+ indicator Rhod2-AM (Abcam, ab142780, 1μm) following the manufacturer’s guidelines. Cells were washed three times with PBS and then were

(17) Morroniside Protects C2C12 Myoblasts from Oxidative Damage Caused by ROS-Mediated Mitochondrial Damage and Induction of Endoplasmic Reticulum StressBiomolecules & TherapeuticsMay 1, 2024Hyun Hwangbo, Cheol Park, EunJin Bang, Hyuk Soon Kim, Sung-Jin Bae, Eunjeong Kim, Youngmi Jung, Sun-Hee Leem, Young Rok Seo, Su Hyun Hong, et al.M36008, Thermo Fisher Scientific), and Rhod-2 AM, fluorescent mitochondrial Ca2+ indicator (Cat. No. ab142780, Abcam, Inc.) in accordance with each manufacturer’s recommendations (Duan et al., 2023). GSH measurement Cells cultured

(18) Mitochondrial dysfunction induced by HIF-1α under hypoxia contributes to the development of gastric mucosal lesionsClinical and translational medicineApril 1, 2024Xiao Y, Liu X, Xie K, Luo J, Zhang Y, Huang X, Luo J, Tan S.mitochondrial Ca2+ concentration ([Ca2+]m) was examined by Rhod‐2AM (a fluorescent Ca2+ indicator, ab142780, Abcam). The fluorescence signals were detected with a Leica STELLARIS STED confocal microscope. The

(19) Lyssavirus M protein degrades neuronal microtubules by reprogramming mitochondrial metabolismmBioMarch 13, 2024Yueming Yuan, An Fang, Haoran Wang, Caiqian Wang, Baokun Sui, Jianqing Zhao, Zhen F. Fu, Ming Zhou, Ling Zhaoprobe The Ca2+ testing of N2a cells was performed using a Fluo-4 AM (S1060, Beyotime) or a Rhod-2 AM (ab142780, Abcam) according to the manufacturer’s instructions. Briefly, the cultured cells to be tested were

(20) A Dual Role of Mesenchymal Stem Cell Derived Small Extracellular Vesicles on TRPC6 Protein and Mitochondria to Promote Diabetic Wound HealingACS nanoFebruary 13, 2024Wang M, Yang D, Li L, Wu P, Sun Y, Zhang X, Ji C, Xu W, Qian H, Shi H.Measurement Mitochondrial Ca2+ uptake was measured using Rhod-2 AM, a fluorescent Ca2+ indicator (Abcam, #ab142780-1 mg). A total of 8 × 104 cells were seeded onto the coverslips and subjected to different treatments.

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Rhod-2 AM, fluorescent Ca2+ indicator from Abcam

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Rhod-2 AM, fluorescent Ca2+ indicator from Abcam

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