Description
MV1 is an antagonist of IAP (inhibitor of apoptosis protein), leads to protein knockdown of HaloTag-fused proteins when combined with HaloTag ligand.In VitroMV1 (0.08-20 µM; 24 h) inhibits the growth of EVSAT cells. MV1 (5 µM; 0-60 min) treatment causes rapid loss of c-IAP1 and c-IAP2 in MDA-MB-231 cells. MV1 (5 µM; 1 h) treatment inducing degradation of c-IAP1 and c-IAP2 is dependent on proteasomal machinery but not on caspase activation. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: EVSAT cells Concentration: 0.08-20 µM Incubation Time: 24 hours Result: Showed IC 50 value of 5 µM for EVSAT cells. Western Blot AnalysisCell Line: MDA-MB-231 cells Concentration: 5 µM Incubation Time: 0-60 min Result: Showed the decreasement of c-IAP1 and 2 protein levels by as early as two minutes following exposure.Form:Solid