Description
L67 (DNA Ligase Inhibitor) is a competitive DNA ligase inhibitor that effectively inhibits DNA ligases I/III (both IC 50 are 10 µM). L67 (DNA Ligase Inhibitor) can cause nuclear DNA damage by reducing levels of mitochondrial DNA and increasing levels of mitochondrially-generated ROS. L67 (DNA Ligase Inhibitor) also activates the Caspase 1 -dependent apoptosis pathway in cancer cells, can be used in cancer research.In VitroL67 (10, 15 µM; 24 h) promotes nuclear DNA damage and (0-50 µM) increases level of mSOX by inhibiting mitochondrial LigIIIα in HeLa cells. L67 (10 µM; 24 h) induces changes in mitochondrial function that causes a reduction in OCR and mitochondrial DNA, and abnormal mitochondrial morphology in HeLa. L67 (10, 100 µM; 24 h) induces apoptosis in cancer cells. L67 (0-30 µM; 24 h) selectively induces cell death in cancer cells by activating a caspase 1-dependent apoptotic pathway. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: HeLa cells Concentration: 10, 15 µM; 0-50 µM Incubation Time: 24 h Result: Increased the formation of nuclear γH2AX foci and steady state levels of γH2AX when at 10 or 15 µM. (γH2AX: a sign of DNA double-strand breaks). Resulted in a concentration (0-50 µM)-dependent increase in mSOX (mitochondrial superoxide) levels. (mSOX is a major cause of the cellular oxidative damage). Cell Viability AssayCell Line: HeLa cells Concentration: 10 µM Incubation Time: 24 h Result: Reduced oxygen consumption rate (OCR) approximately 20%. Resulted in about a 25% reduction in mitochondrial DNA. Apoptosis AnalysisCell Line: HeLa cells Concentration: 10, 100 µM Incubation Time: 24 h Result: Result:Induced apoptosis, and at 100 µM with apoptotic cells constituting about 50% of the HeLa cell population. Cell Viability AssayCell Line: HeLa cells Concentration: 0-30 µM Incubation Time: 24 h Result: Activates a caspase 1-dependent cell death pathway in cancer cells