293T cells transfected with overexpressed human IL-17a were incubated with Protein TransportInhibitor MIX for 4 h after 24 h in a CO2 incubator at 37℃, fixed, permeabilized, and then stained with IL-17A PE.
Mouse spleen cell were stimulated with Cell Stimulation MIX and Protein Transport Inhibitor MIX for 5 hours, fixed, permeabilized, and then stained with CD3 APC and IFN-γ FITC.
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