Fluo-4 AM from MedChemExpress

Product Specs
ItemFluo-4 AM
CompanyMedChemExpress
Price $150.00Supplier Page View Company Product Page
Catalog NumberHY-101896
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
Purity≥99.0%
FormulaC51H50F2N2O23
Research AreaOthers
TypeChemical
Molecular Weight1096.94
Quantity100 µg
Molecule NameFluo-4 AM

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(1) Bellidifolin Improves Pulmonary Artery Smooth Muscle Cells Proliferation by Targeting the IGFBP5-Mediated PI3K-AKT-mTOR Pathway and Dilates the Pulmonary ArteryBiomoleculesJuly 19, 2026Qiuqin Hu, Hongmai Wang, Yujie Qiao, Qingqing Xia, Jiacheng Hu, Xiangyun Gai, Yulin Li, Tao Chen, Zhanqiang LiTiangen Biochemical Technology Co., Ltd. (Beijing, China). KC7F2 (Cat# HY-18777) and Fluo-4-AM (Cat# HY-101896) were purchased from MedChemExpress (Shanghai, China). BCA Protein Assay Kit was purchased from Boster

(2) Dracocephalum moldavica L. Flavonoids Alleviate Doxorubicin-Induced Cardiotoxicity by Activating the AMPK/PGC1αPathway to Preserve Mitochondrial HomeostasisInternational Journal of Molecular SciencesJune 23, 2026Ruifang Zheng, Yanwen Du, Shoubao Wang, Wenling Su, Kaderyea Kader, Lijuan Zhang, Zihan Wang, Diwei Liu, Jianguo Xing, Shifeng Chu, et al.Intracellular Ca2+ Content H9c2 Cell pellets following two PBS washes were incubated with 4 μΜ Fluo-4 AM (HY-101896, MCE, Shanghai, China) for 20 min at 37 °C under protection from light. Following 3 washes with chilled

(3) The MCU-MECOM Axis Orchestrates Glioblastoma Progression by Remodeling Mitochondrial Dynamics and Quality Control via MAMsInt J Biol SciMay 1, 2026Xiaodong Li, Yaoliang Wang, Feifei Wu, Xin Huang, Ziwei Ni, Guangzhen Xu, Weizhong Wang, Jingjing Tie, Yunqiang Huang, Yuze Sun, et al.calcium and ER calcium in primary cells, U87MG, and GSCs were detected by using the indicator Rhod-2 AM (HY-101896, MedChemExpress, USA) and the indicator Mag-Fluo-4 AM (HY-D1498, MedChemExpress, USA) respectively (detailed

(4) ALKBH3 m1A Demethylase Deficiency Reduces Alzheimer's Amyloid‐β PathologyAdvanced ScienceMarch 12, 2026Yueyang Li, Sifei Yu, Kaidong Lu, Yujie Zhang, Mingjie Dong, Yan Peng, Liang Xue, Waleed Alam, Yuxuan Shui, Yi Zhou, et al.in R (v4.5). 4.12 Fluo‐4AM Assay Calcium imaging in SH‐SY5Y cells was performed using Fluo‐4AM (#HY‐101896, MCE, USA). Prior to experiments, a 1 mM stock solution of Fluo‐4AM was prepared in anhydrous DMSO,

(5) Simvastatin Alleviates ConA-Induced Autoimmune Hepatitis by Inhibiting CD4+ T Cell Activation via Calcium-NFATC3 PathwayInflammationJanuary 30, 2026Xiyu Wang, Tianhang Long, Longyang Zhou, Nan Xu, Peiyang Fang, Buer Li, Haozhe Xu, Guangyong Sun, Dong Zhang, Hua JinDetection Intracellular calcium concentration in CD4+ T cells was evaluated using the Fluo-4 AM fluorescent probe (HY-101896, MCE, USA). Briefly, the cells were resuspended in PBS at a density of 2 × 106/ml and incubated with

(6) Senotoxins target senescence via lipid binding specificity, ion imbalance and lipidome remodelingNature AgingJanuary 12, 2026Javier Moral-Sanz, Isabel Fernández-Carrasco, Valentina Ramponi, Amanda Garrido, Izhar Karbat, Pablo Cabezas-Sainz, Esperanza Rivera-de-Torre, Osama Elsallabi, Roberto Martín-Hernández, José L. López-Aceituno, et al.10.1.0. Determination of basal content of free calcium Intracellular free calcium was measured using Fluo-4AM (HY-101896, MedChemExpress). Cells were stained with 1 µM Fluo-4AM and 0.02% poloxamer 407 (HY-D1005, MedChemExpress)

(7) Gastrodin alleviates high fructose-induced podocyte mitochondria-mediated apoptosis by inhibiting NLRP6 to facilitate TRIM7-triggered Bok mRNA degradationInternational Journal of Biological SciencesJanuary 1, 2026Hong Ding, Wen-Xuan Wang, Qiong-Dan Liang, Chuan-Feng Tang, Tang-Di Xu, Zi-An Miao, Bang-Xing Han, Ling-Dong Kong(Ca2+) flux The intracellular Ca2+ concentration of podocytes were measured by the Fluo-4 AM probe (HY-101896, MedChemExpress). 1 × 105 cells were gathered and stained 1 μM working solution (Ca2+-free HBSS) at

(8) Glycine-GLRA1-calmodulin signaling regulates endoplasmic reticulum calcium to sustain insulin secretion and β-cell functionLife MetabolismDecember 19, 2025Jiarui Zhang, Zehui Cao, Jinbao Yang, Kuo Zhu, Shuai Liu, Weijie Liu, Jin Lu, Hongfang Zhao, Wei Dai, Chunmei Chang, et al.Biotechnology, Mag-Fluo-4-AM (20401) from AAT Bioquest, Bitopertin (HY-10809), W7 (HY-100912), Fluo-4AM (HY-101896) from MedChemExpress, streptozocin (STZ, A610130) from Sangon Biotech, insulin from Novo Nordisk, glucose

(9) Exploiting eNOS activation to achieve tumor vascular normalization via endothelial transcytosis of lipid nanoparticlesActa Pharmaceutica Sinica BDecember 3, 2025Huisong Hao, Yunfei Yi, Yanan Fu, Shengjie Sun, Zhangwen Peng, Jia Tang, Yixuan Fang, Shihao Zhuang, Yaqi Ouyang, Tianqi Wang, et al.PBS. The intracellular Ca2+ and H2O2 levels were detected by CLSM after being stained by Fluo-4 AM (HY-101896, MCE) and ROSGreen H2O2 Probe (MX5202, MKBio), respectively. 2.15 Intracellular hypoxia relief The

(10) Novel intranasal delivery of sihosogansan demonstrates rapid antidepressant activity via GABAergic and BDNF/TrkB pathways: identification of potential bioactive quality markersChinese medicineOctober 6, 2025Tran KN, Munni YA, Nguyen LTH, Oh TW, Choi HJ, Moon IS, Yang IJ.After 48 h of incubation, cells were rinsed with 1 × DPBS and treated with 1 μM Fluo-4 (MedChemExpress, HY-101896) for 40 min at 37 °C. Subsequently, the cells were rinsed twice with 1 × DPBS and observed under a fluorescence microscope

(11) Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunityJ Immunother CancerOctober 5, 2025Haiyang Yu, Xin Sun, Yan Li, Jing Pan, Xuemei Liu, Hongbin He, Haibo Wu, Yubei Sun, Yueyin Pan, Xiaojun Qian(Xiamen University, Xiamen, China). MCC950 was purchased from Selleck. MnTBAP (HY-126397), Fluo-4 (HY-101896), ML-SI3 (HY-139426), 2-APB (HY-W009724), and BAF-A1 (HY-100558) were purchased from MedChemExpress.

(12) Identification of Phialomustin-B (PHL-B) as a Novel Natural TRPA1 Channel InhibitorACS OmegaSeptember 2, 2025Priyanka Yadav, Ashutosh Sharma, Aditya Singh, Mansi Sharma, Appu Kumar Singh, Asif Ali, Aravind Singh Kshatricells were seeded on 12 × 12 mm glass coverslips for 24 h before being loaded with 5 μM Fluo-4 AM (HY-101896, MedChemExpress) in standard buffer solution (SBS) for 30 min in 5% CO2 at 37 °C. To prevent dye compartmentalization

(13) SETD2 drives METTL14-mediated m6A to suppress Piezo1 Attenuation and activate TGM2 to promote pulmonary hypertensionCell Mol Life SciAugust 8, 2025Shuai-Shuai Zhao, Chuan Yuan, Jin-Long Liu, Qi-Cai Wu, Xue-Liang ZhouChemiDoc MP imaging system. Ca2+ detection A solution of 1 mg of Fluo-4 acetoxymethyl ester (Fluo-4 AM) (HY-101896, MCE, China) in 442 µl of dimethyl sulfoxide (DMSO) was prepared to a concentration of 2 mM, and 16.5

(14) Lactobacillus johnsonii Generates Cyclo(pro‐trp) and Promotes Intestinal Ca2+ Absorption to Alleviate CKD–SHPTAdvanced ScienceJanuary 31, 2025Xiong Zeng, Lihua Sun, Huichao Xie, Shenhai Gong, Caibao Lu, Zhongwei Xu, Haidi Guan, Ben Han, Wei Wang, Zhengmin Zhang, et al.according to the manufacturer's instructions. In vitro Ca2+ imaging: [Ca2+]i was measured using Fluo‐4 AM (HY‐101896, MCE) as previously described.[ 89 ] MODE‐K cells were cultured in RPMI 1640 medium. One day later,

(15) Low expression of CCKBR in the acinar cells is associated with insufficient starch hydrolysis in ruminantsCommunications BiologyDecember 20, 2024Yan Cheng, Tianxi Zhang, Chao Yang, Kefyalew Gebeyew, Chengyu Ye, Xinxin Zhou, Tianqi Zhang, Ganyi Feng, Rui Li, Zhixiong He, et al.Fluo-4 by preincubation in 5 μM Fluo-4-am (working fluids: 100 μg Fluo-4-am and 18.2325 ml PBS; MCE HY101896) for 30 min at 37 °C. After 1 minute of exposure, Fluo-4-acinar cells were loaded with a physiologically

(16) Calcium influx: An essential process by which α-Synuclein regulates morphology of erythrocytesJournal of advanced researchAugust 1, 2024Yang Y, Shi M, Liu X, Zhu Q, Xu Z, Liu G, Feng T, Stewart T, Zhang J.RBCs were washed with Ringer buffer and then incubated with 2 μM of the cell-permeable Fluo-4 AM dye (HY-101896, MCE) in Ringer buffer at 37 °C for 1 h. Subsequently, RBCs were treated with 0.15 μg/ml, 0.75 μg/ml,

(17) SLC24A-mediated calcium exchange as an indispensable component of the diatom cell density-driven signaling pathwayThe ISME JournalMarch 8, 2024Xuehua Liu, Zhicheng Zuo, Xiujun Xie, Shan Gao, Songcui Wu, Wenhui Gu, Guangce WangSolution (HBSS, without Ca2+, Mg2+, and phenol red). Then, the cells were loaded with Fluo-4-AM (1 μM) (HY-101896, MCE) for 1 h at 20°C. After the dye loading, the cells were rinsed with HBSS for three times, then

(18) Enhanced macromolecular substance extravasation through the blood-brain barrier via acoustic bubble-cell interactionsUltrasonics SonochemistryFebruary 1, 2024Jifan Chen, Jean-Michel Escoffre, Oliver Romito, Tarik Iazourene, Antoine Presset, Marie Roy, Marie Potier Cartereau, Christophe Vandier, Yahua Wang, Guowei Wang, et al.generated by acoustically stimulated cells. 2.10 Cytosolic Ca2+ and Ca2+ related pathway Fluo-4-AM (HY-101896; MedChemExpress, USA) was used to detect the relative cytosolic Ca2+ concentration change. The working

(19) DNA methylation-dependent epigenetic regulation of Verticillium dahliae virulence in plantsaBIOTECHSeptember 20, 2023Yun-Ya Chen, Chen Zhu, Jian-Hua Zhao, Ting Liu, Feng Gao, Ying-Chao Zhang, Cheng-Guo Duanas previously described (Zhao et al. 2016). In brief, a stock solution of Fluo-4AM (MedChemExpress, HY-101896), 4 mM in dimethyl sulfoxide (DMSO), was prepared and diluted with double distilled water to make a

(20) Berbamine promotes macrophage autophagy to clear Mycobacterium tuberculosis by regulating the ROS/Ca 2+ axismBioJune 29, 2023Su Zhang, Xuefeng Zhou, Min Ou, Xiangdong Fu, Qiao Lin, Xiaoyu Tao, Zhaoqin Wang, Aimei Liu, Guobao Li, Yuzhong Xu, et al.level of cytoplasmic Ca2+ (cyto-Ca2+) was measured by the fluorescent probe Fluo-4 AM (MedChemExpress, HY-101896), the level of mitochondrial Ca2+ (mito-Ca2+) was measured by the fluorescent dye Rhod-2 AM (MedChemExpress,

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Fluo-4 AM from MedChemExpress

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Fluo-4 AM from MedChemExpress

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