Description
A withanolide steroidal lactone with anti-inflammatory and anticancer activities; inhibits LPS-induced NO production in RAW 264.7 cells and TNF-α-induced NF-κB activity in HEK293 cells (IC50s = 0.32 and 0.04 µM, respectively); inhibits LPS-induced increases in iNOS and COX-2 levels and Akt and STAT1 phosphorylation in RAW 264.7 cells at 5 µM; inhibits Wnt signaling in HCT116 and SW480 colorectal cancer cells (IC50s = 1.85 and 2.67 µM, respectively); inhibits the proliferation of HCT116, SW480, HT-29, and LoVo cells (IC50s = 0.24-0.51 µM); halts the cell cycle at the G0/G1 phase in HCT116 and HT-29 cells and induces apoptosis in HCT116 and SW480 cells; reduces tumor growth in an HCT116 mouse xenograft model at 10 mg/kg per day for 14 days