Calcein-AM from BioLegend

Product Specs
ItemCalcein-AM
CompanyBioLegend
Price $165.00Supplier Page View Company Product Page
Catalog Number425201
Quantity10X50UG
ApplicationsFC

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Human bronchial air-liquid interface responses to nebulised protease exposureFront ImmunolAugust 7, 2026Georgina Hopkins, David Sheffield, Hugh Barlow, Anja Lalljie, Victoria James, Stella Cochrane, Lucy C. Faircloughremove any cells. 40 μL of supernatants were then immediately incubated with 10 μM calcein AM (Cat no. 425201, Biolegend), 2.5μg/mL of tetraspanins CD9 APC (Clone REA1071, Cat no. 130-118-808, RRID: AB_2733406,

(2) Impact of liquid dosing and associated cell ‘drowning’ in a 3D human bronchial epithelial model used for protease exposure studiesFrontiers in ToxicologyMay 27, 2026Georgina Hopkins, David Sheffield, Hugh Barlow, Anja Lalljie, Stella Cochrane, Lucy C Faircloughcells. Aliquots (40 μL) of supernatants were then immediately incubated with 10 μM calcein AM (Cat no. 425201, Biolegend), 2.5 μg/mL of tetraspanins CD9 APC, CD63 PE, and CD81 PEVio615 (Miltenyi) for 1 h at 37

(3) Modelling kidney cystogenesis using human kidney tubuloid culturesBMC Mol Cell BiolMay 11, 2026Jana Rohe, Arsila Palliyulla Kariat Ashraf, Melina Kehl, Xenia Thielmann, Jörg Ellinger, Glen Kristiansen, Dagmar Wachten, Marieta Ioana Tomain suspension tubuloids, dissociated single cells were incubated with 2 µM Calcein-AM (Biolegend, #425201) for 30 min at 37 °C under 5% CO2. Following, cells were seeded in a ULA 96-well multi-well plate at

(4) Genome-wide CRISPR screens in primary human natural killer cells identify countermeasures against immunosuppressive environmentNature CommunicationsApril 15, 2026Quoc Viet Nguyen, Yi-Jun Lan, Jason Cheng-Yu Chang, Hsin-An Shih, Jenifer Faustine, Cheng-Chieh Chen, Shu-Yu Ho, Ching-Wen Cheng, Tsu-Lan Chao, Steven Linresuspended at 1 × 106 cells/mL in Complete DMEM containing 1.6 μL of 10 μM Calcein-AM (BioLegend # 425201) and incubated at 37 °C for 30 min. After staining, cells were washed three times with DPBS and adjusted

(5) Synaptic proteins that aggregate and degrade slower with aging accumulate in microgliabioRxivApril 12, 2026Ian H. Guldner, Viktoria P. Wagner, Patricia Moran-Losada, Sophia M. Shi, Kelly Chen, Barbara T Meese, Hamilton Oh, Yann Le Le Guen, Nannan Lu, Pui Shuen Wong, et al.1:10 with APC/Cyanine7 anti-mouse/human CD11b Antibody (BioLegend, 101225) and Calcein-AM (BioLegend, 425201) at a final concentration of 1x in Cell Staining Buffer (BioLegend, 420201) for 30 minutes on ice. Cells

(6) Ageing promotes microglial accumulation of slow-degrading synaptic proteinsNatureFebruary 1, 2026Guldner IH, Wagner VP, Moran-Losada P, Shi SM, Golub SW, Hevler JF, Chen K, Meese BT, Ghoochani A, Pulido E, et al.1:10 with APC/cyanine7 anti-mouse/human CD11b antibody (BioLegend, 101225) and calcein-AM (BioLegend, 425201) at a final concentration of 1× in cell staining buffer (BioLegend, 420201) for 30 min on ice. Cells

(7) PURE-seq integrates FACS and PIP-seq for single-cell genomics of ultra-rare cellsNature CommunicationsJanuary 21, 2026Sixuan Pan, Inés Fernández-Maestre, Kai-Chun Chang, Stéphane Van Haver, Matthew G. Wereski, Alexandra M. Haugh, Katy K. Tsai, Adil I. Daud, Robert L. Bowman, Harish N. Vasudevan, et al.human TruStain FcX (Biolegend, 422301), APC anti-human CD45 (Biolegend, 304011), and Calcein AM (Biolegend, 425201). After staining, the cells were washed once with Cell Staining Buffer (Biolegend, 420201) and filtered

(8) Rbfox2 selectively governs hematopoietic stem cell self-renewal by regulating proteostasisSci AdvDecember 5, 2025Longfei Gao, Desmond Dickson, Huijuan Feng, Chaolin Zhang, Lei Dingwere washed twice with PBS. Cells were then stained with 0.5 μM calcein-AM (BioLegend, catalog no. 425201) for 5 min on ice. After being washed with PBS, the cells were analyzed with flow cytometry. Live cells

(9) HERPUD1 mediates palmitic acid-induced UPR sustaining TNBC aggressiveness and is destabilized by CK2 pharmacological inhibitionCell death & diseaseNovember 5, 2025Hernández-Torres L, Cavieres VA, Cortés O, Arias-Muñoz E, Cruzat-Arias F, Catalán-Aguilera J, Álvarez-Indo J, Aguilera-Olguín M, Hernández R, Silva-Pavez E, et al.Sytox (cat#10768273) were purchased from Invitrogen (Carlsbad, CA, United States). Calcein-AM (cat#425201) and FITC Annexin V Apoptosis Detection Kit with 7-AAD (cat#640922) were purchased from BioLegend (San

(10) The Contribution of CD26-Negative Fibroblasts to Endometrial ScarringBiomoleculesOctober 10, 2025Riaz MA, Pecher CM, Kary FL, Maoga JB, Dietze R, Zeppernick F, Meinhold-Heerlein I, Konrad L.Cell signaling technology); TrypLE enzyme (cat-no. 12604-021; ThermoFisher Scientific); Calcein-AM (cat-no. 425201; BioLegend; San Diego, CA, USA); Diprotin A (DPA, cat-no. ALX-260-036-M025; Enzo Life Sciences, New

(11) Rapid generation of functional vascular organoids via simultaneous transcription factor activation of endothelial and mural lineagesCell Stem CellAugust 7, 2025Liyan Gong, Yadong Zhang, Yonglin Zhu, Umji Lee, Allen Chilun Luo, Xiang Li, Xi Wang, Danyang Chen, William T. Pu, Ruei-Zeng Lin, et al.solidified Matrigel in EGM-2 medium. After 6 hours, cells were incubated with 1 μM Calcein-AM (BioLegend, 425201) for 10 minutes and imaged using a fluorescence microscope. Branch numbers were quantified with ImageJ. Leukocyte

(12) FOXA2 promotes metastatic competence in small cell lung cancerNat CommunMay 26, 2025Kenta Kawasaki, Sohrab Salehi, Yingqian A. Zhan, Kevin Chen, Jun Ho Lee, Eralda Salataj, Hong Zhong, Parvathy Manoj, Dennis Kinyua, Barbara P. Mello, et al.materials were FACS-sorted with DAPI (Thermo Fischer Scientific, Cat#:D1306), Calcein AM (Biolegend Cat#: 425201), PE anti-CD45 antibody (working dilution 3uL/100uL, Biolegend, Cat#: 368510) and processed for scRNA-seq

(13) Actionable heterogeneity of hepatocellular carcinoma therapy-induced senescenceCancer Immunol ImmunotherMay 15, 2025Pujan Engels, Andras Szolek, Sebastian Hörner, Georgios Vavouras Syrigos, Kim Hebbel, Michelle Schmidtke, Min Zhou, Maria Mateo-Tortola, Caroline Schönfeld, Sylwia Anna Stefanczyk, et al.NK cell-mediated cytotoxicity a calcein acetoxymethyl esther (calcein AM) assay was performed (Biolegend, 425201). Prior to the calcein AM assay, target cells were seeded in a 24-well plate and senescence was induced

(14) The Zika virus NS5 protein binds HSP90 to suppress EGF-induced Akt signaling and trophoblast cell migrationVirologyFebruary 1, 2025Nika Hajari, Megan Knoll, Amy Lu, Isaac Barber-Axthelm, Michael Galehours for migration to happen. Cells were then stained with a viability dye, Calcein-AM (Biolegend, no. 425201, 1:1000), in HBSS (Thermofisher, no. 14025092). Confocal immunofluorescence images of live cells from

(15) Inhibiting ADAM17 enhances the efficacy of olaparib in ovarian cancer spheroidsScientific ReportsNovember 6, 2024Christoph Rogmans, Jan Dittrich, Emily Hamm, Jörg Paul Weimer, David Holthaus, Norbert Arnold, Inken Flörkemeier, Nicolai Maass, Peer Jansen, Astrid Dempfle, et al.after treatment. Dye was applied to the wells to stain living cells with 0,1 µg/ml of Calcein-AM (BioLegend, #425201) and dead cells with 5 µg/mL of propidium iodide (PI) (BioLegend, #421301). As a nuclear counterstain

(16) Novel 3-D Macrophage Spheroid Model Reveals Reciprocal Regulation of Immunomechanical Stress and Mechano-Immunological ResponseCellular and Molecular BioengineeringOctober 14, 2024Alice Burchett, Saeed Siri, Jun Li, Xin Lu, Meenal DattaActivity Assays For live/dead staining, the gels were incubated with 2 μg/ml Calcein-AM (BioLegend, 425201) and 1 μg/ml Propidium Iodide (PI, MP Biomedicals, 0219545810) in complete medium for 30 min at 37 °C.

(17) Mucosal‐associated invariant T cells modulate innate immune cells and inhibit colon cancer growthScandinavian Journal of ImmunologySeptember 1, 2024Olivia J. Cheng, Eric J. Lebish, Owen Jensen, Damian Jacenik, Shubhanshi Trivedi, Jackson G. Cacioppo, Jeffrey Aubé, Ellen J. Beswick, Daniel T. Leungμg/ml. COLO 205 Killing Assays For Calcein Release Assay, 50,000 Calcein-AM-labeled (2 μM, Cat. #: 425201, Biolegend, San Diego, CA, USA) COLO 205 cells were co-cultured with 500,000 human whole leukocytes

(18) cAMP signaling of Bordetella adenylate cyclase toxin blocks M-CSF triggered upregulation of iron acquisition receptors on differentiating CD14 + monocytesmSphereAugust 28, 2024Jawid Nazir Ahmad, Peter SeboDNase I (Cat. number M0303) were from New England Biolabs. Calcein-AM was from Biolegend (Cat. number 425201), and trichostatin A was from MedChemExpress (Cat. number HY-15144). Monocyte separation and analysis

(19) Hematopoietic stem cell niche generation and maintenance are distinguishable by an epitranscriptomic programCellMay 23, 2024Gao L, Lee H, Goodman JH, Ding L.stained with anti-CD45 (30-F11), Ter119, and anti-PDGFRα (APA5) antibodies. Calcein-AM (BioLegend, Cat#425201) was applied to exclude dead cells during sorting for single-cell RNA sequencing. Osteolineage cells

(20) Nucleoporin93 limits Yap activity to prevent endothelial cell senescenceAging cellApril 1, 2024Nguyen TD, Rao MK, Dhyani SP, Banks JM, Winek MA, Michalkiewicz J, Lee MY.monocytes (2 × 105 cells/mL) were labeled with 5 μM cell‐permeant Calcein‐AM in DMSO (1:200, BioLegend, 425201) for 20 min at 37°C, centrifuged, and then resuspended in an endothelial cell growth medium. Labeled

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Calcein-AM from BioLegend

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