NEB ExpressIqCompetentE. coli(High Efficiency) from New England Biolabs

Product Specs
ItemNEB ExpressIqCompetentE. coli(High Efficiency)
CompanyNew England Biolabs
Price $190.00 Inquire
Catalog NumberC3037I
Quantity6 x 0.2 ml
GenotypefhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10--Tet^S)2 [dcm] R(zgb-210::Tn10--Tet^S) endA Δ(mcrC-mrr)114::IS10 [miniF-lacI^q Cam^R]
ApplicationsProteomics & Glycomics
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
SpeciesE. coli
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
2-6 x 10⁸ cfu/µg pUC19 DNA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applicationsNature CommunicationsMay 28, 2026Pao-Wan Lee, Seyed Saeed Mottaghi, Matthis Guillaume Lugnier, Sebastian J. Maerklvariants were cloned into the pQE30 vector and transformed into NEBExpress® Iq Competent E. coli(NEB, C3037I) for protein expression. The plasmid encoding ZF-NB23 was cloned into pET-21(+) and transformed into

(2) Comparative characterization of Cas12f orthologs reveals mechanistic features underlying enhanced genome editing efficiencyNature Structural & Molecular BiologyApril 13, 2026Kaoling Guan, Rodrigo Fregoso Ocampo, Paula B. Matheus Carnevali, Cindy J. Castelle, Liliana Gonzalez-Osorio, Dominic T. Castanzo, Nicole C. Thomas, Molly Brothers, Tyler L. Dangerfield, Matthew M. Hooper, et al.production Al3Cas12f proteins were expressed in the pMGB∆ vector. E. coli (NEBExpress Iq Competent E. coli; NEB, C3037I) cultures were grown at 37 °C in 2× YT medium (1.6% tryptone, 1% yeast extract and 0.5% NaCl) or Terrific

(3) Uropathogenic Escherichia coli invade luminal prostate cells via FimH–PPAP receptor bindingNature MicrobiologyJanuary 8, 2026Maria Guedes, Simon Peters, Amruta Joshi, Sina Dorn, Janina Rieger, Kimberly Klapproth, Tristan Beste, Alexander M. Leipold, Mathias Rosenfeldt, Antoine-Emmanuel Saliba, et al.into the EcoRI-BamHI sites of plasmid pQE-30 and transformed into E. coli T4 Express competent cells (C3037I, New England BioLabs). Expression of fimH-FLAG was induced with 1 mM IPTG (2316.3, Carl Roth) for 5

(4) Autophagy cargo profiles in skeletal muscle during starvation and exerciseAutophagy RepDecember 5, 2025Mohd Farhan, Shangze Lyu, Trezze P. Nguyen, Dakai Zhang, Hong Liang, Yong Zhou, Yu A. An, Jun Wang, Hongyuan Yang, Guangwei Du, et al.single or tandem GFP nanobody protein was expressed in NEBExpress® Iq E. coli (New England Biolab, C3037I) as described previously[59]. Briefly, an 80 ml pre-culture of the E. coli containing the expression

(5) Biophysics-based protein language models for protein engineeringNature MethodsSeptember 11, 2025Sam Gelman, Bryce Johnson, Chase R. Freschlin, Arnav Sharma, Sameer D’Costa, John Peters, Anthony Gitter, Philip A. Romerocodons. Clonal genes ordered from Twist Biosciences were transformed into NEBExpress Iq Competent E. coli (NEB, C3037I) cells and plated on Luria Broth plates with carbenecillin selection (0.1 mg ml−1). Proteins were expressed

(6) DNA Hanger: Surface‐Minimized Single‐Molecule Immunoassay PlatformSmallJune 8, 2025Jincheol Seol, Byungju Kim, Eui‐Sang Yu, Cherlhyun Jeong, Jong‐Bong Leesequence in the plasmid. Recombinant proteins were overexpressed in NEBExpress Iq Competent E. coli (C3037I, NEB) cultured in LB medium. Protein expression was induced by adding 0.4 mm IPTG, and the culture was

(7) Immobilization of KR-12 on a Titanium Alloy Surface Using Linking Arms Improves Antimicrobial Activity and Supports Osteoblast CytocompatibilityACS Applied Bio MaterialsApril 21, 2025Mohadeseh Zare, Laura Colomina Alfaro, Antonella Bandiera, Esra Cansever Mutlu, David Grossin, Fernando Albericio, Sarah A. Kuehne, Zubair Ahmed, Artemis Stamboulisconditions, as described in the literature.34,35 Briefly, recombinant clones of the Escherichia coli C3037I strain (New England Biolabs, Ipswich, MA) transformed with the plasmid carrying HELP DNA sequences were

(8) Targeting protein-ligand neosurfaces with a generalizable deep learning toolNatureMarch 1, 2025Marchand A, Buckley S, Schneuing A, Pacesa M, Elia M, Gainza P, Elizarova E, Neeser RM, Lee PW, Reymond L, et al.using Gibson assembly. The plasmid was then transformed into NEBExpress Iq competent E. coli (NEB, C3037I) for protein expression. Bacteria were precultured overnight and inoculated to a 500 ml Luria-Bertani

(9) Biophysics-based protein language models for protein engineeringbioRxivJanuary 14, 2025Sam Gelman, Bryce Johnson, Chase Freschlin, Arnav Sharma, Sameer D'Costa, John Peters, Anthony Gitter, Philip A Romerocodons. Clonal genes ordered from Twist Biosciences were transformed into NEBExpress Iq Competent E. coli (NEB C3037I) cells and plated on Luria Broth (LB) plates with carbenecillin selection (0.1 mg/mL). Proteins were

(10) A Versatile Elastin‐Like Carrier for Bioactive Antimicrobial Peptide Production and DeliveryMacromolecular BioscienceMarch 1, 2024Laura Colomina – Alfaro, Paola Sist, Silvia Marchesan, Ranieri Urbani, Artemis Stamboulis, Antonella Bandierasites.[ 12 ] The positive clones were sequenced to verify the open reading frame and then the E. coli C3037I (New England Biolabs Inc., Ipswich, MA) expression strain was transformed with the plasmid carrying

(11) Human Cytomegalovirus IE2 Both Activates and Represses Initiation and Modulates Elongation in a Context-Dependent MannermBioJune 28, 2022Christopher B. Ball, Ming Li, Mrutyunjaya Parida, Qiaolin Hu, Deniz Ince, Geoffrey S. Collins, Jeffery L. Meier, David H. Priceagarose gel electrophoresis and Sanger sequencing. Vectors were transformed into NEB Express BL21 (NEB C3037I) and selected on ampicillin agar plates overnight at 37°C. Colonies were picked up the following morning

(12) Correction of frameshift mutations in the atpB gene by translational recoding in chloroplasts of Oenothera and tobaccoThe Plant CellJuly 2, 2021Irina Malinova et al.restriction sites. Final constructs were transformed into NEBExpress chemically competent E. coli cells (C3037I, NEB). Cultures of three individual clones were grown at 37°C in LB medium supplemented with 100 µg

(13) WNK1 is an assembly factor for the human ER membrane protein complexMolecular cellJuly 1, 2021Pleiner T, Hazu M, Tomaleri GP, Januszyk K, Oania RS, Sweredoski MJ, Moradian A, Guna A, Voorhees RM.BL21 Competent E. coli,New England Biolabs,Cat# C2530H NEBExpress® Iq Competent E. coli,New England Biolabs,Cat# C3037I Oligonucleotides,Oligonucleotides,Oligonucleotides On-Targetplus siRNA against WNK1: GCAGUUGUCUCAAUAUCUA,Dharmacon,Cat#

(14) Streptococcus pneumoniae binds to host GAPDH on dying lung epithelial cells worsening secondary infection following influenzaCell ReportsJune 15, 2021Sang-Sang Park, Norberto Gonzalez-Juarbe, Ashleigh N. Riegler, Hansol Im, Yvette Hale, Maryann P. Platt, Christina Croney, David E. Briles, Carlos J. OrihuelaHui Wu Lab, University of Alabama at Birmingham",PMC6763767 NEB®Express Iq Competent E. coli,NEB,Cat#C3037I E. coli strain BL21(DE3),Novagen,Cat#69450-4 E. coli strain NovaBlue,Novagen,Cat#70181-3 "Influenza A virus

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