NEB 5-alpha Competent E. coli (Subcloning Efficiency) from New England Biolabs

Product Specs
ItemNEB 5-alpha Competent E. coli (Subcloning Efficiency)
CompanyNew England Biolabs
Price $73.00 Inquire
Catalog NumberC2988J
Quantity6 x 0.4 ml
GenotypefhuA2 ?(argF-lacZ)U169 phoA glnV44 F80? (lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
ApplicationsDNA Manipulation
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
SpeciesE. coli
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
> 1 x 10⁶ cfu/µg

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Bacterial-derived cyclic lipopeptides suppress dauer arrest in C. elegansbioRxivSeptember 16, 2026Xiao Wang, Kelsie M. Nauta, Marco E. Mechan-Llontop, Darrick R. Gates, Nirmal Chaudhary, Amudha Ramchandran, Kersten K. Sykes, Corrie L. Sakowski, Vashnie H. Hartwell, Jade Tarango, et al.E2621). Assembled plasmids were transformed into E. coli DH5α competent cells (New England Biolabs, C2988J) according to the manufacturer’s instructions for propagation and sequence verification. All PCR amplified

(2) Structural insight into sodium-dependent bile acid transport by members of the SLC10 familyStructureSeptember 3, 2026Criska Yuanting Li, Aurélien A. Grob, Leah Repa, Oliver Huxley, Deborah H. Brotherton, Patrick Becker, Ruby Dadzie, Oliver Beckstein, Alexander D. Cameronstrains,Bacterial and virus strains NEB 5-alpha Competent E. coli (Subcloning Efficiency),New England Biolabs,C2988J E. coli Lemo21 (DE3),New England Biolabs,C2528H ,, Biological samples,, ,, "Chemicals, peptides,

(3) Productive Mayaro Virus Infection Requires Host Fatty Acid Synthase for nsP1 S-palmitoylationbioRxivJuly 26, 2026Paola N. Loperena González, Adam Brynes, Bryan Perez Soto, Jacqueline Corry, Taanishi Gulati, Hannah Tsui, Nicholas M. Chesarino, Jesse J. Kwiekbetween fragments. pcDNA3.1-Flag-nsP1 constructs were transformed into NEB® 5-alpha Competent E. coli (C2988J, NEB) and IMC constructs were transformed into NEB® Stable Competent E. coli (C3040H, NEB) selected

(4) Mechanistic design of cell-penetrating disruptors for phospho-dependent TACC3-CHC interactionStructure (London, England : 1993)June 4, 2026Gunning V, Batchelor M, Alexander KK, Walko M, Dill TC, Burgess SG, Royle SJ, Kennedy EJ, Bayliss R.Methods EXPERIMENTAL MODEL AND STUDY PARTICIPANT DETAILS DH5-α competent Escherichia coli cells (NEB, C2988J, RRID:SCR_021133) were used for molecular cloning and mutagenesis. BL21(DE3) RIL competent E. coli cells

(5) Development and Optimization of CRISPR/Cas9-Assisted Recombineering in Escherichia albertiiMicroPubl BiolMay 5, 2026Shahab Ahmad Khan, Tara Marie Miller, Shantanu Bhatt, Edwin LirelA1 gyrA96 deoR nupG ϕ80d lacZ ΔM15 Δ( lacZYA-argF ) U169 , hsdR17 (rK− mK+) λ−",New England Biolabs #C2988J LMG20976,E. albertii wild-type strain,Bhatt’s lab EL1001,"LMG20976 transformed with pEcCas, Km R",This

(6) Mitochondrial presequences harbor variable strengths to maintain organellar functionJournal of Cell BiologyApril 6, 2026Youmian Yan, Baigalmaa Erdenepurev, Thiago N. Menezes, Ian Collinson, Natalie M. Niemiligation products were transformed into DH5α competent E. coli cells (New England Biolabs, catalog #C2988J), and plasmids were isolated from transformants. All cloned plasmids were validated by Sanger sequencing. Presequences

(7) The Rpd3 histone deacetylase is a critical regulator of temperature-mediated morphogenesis and virulence in the human fungal pathogen HistoplasmaPLOS BiologyMarch 17, 2026Nebat Ali, Mark Voorhies, Rosa A. Rodriguez, Anita Silstudy are listed in S2 Table. Constructs were generated in E. coli DH5⍺ (New England Biolabs (NEB) C2988J) and OneShot TOP10 (Fisher Scientific C404003) cells. CRISPR/Cas9 plasmids for targeted KO were generated

(8) A One-Step Method for Efficient Purification of Functional Cas9 ProteinBIO-PROTOCOLFebruary 5, 2026Xinzhi Duan, Zhengrong Zhou, Aihua MaoE. coli BL21 (DE3) cells (NEB, catalog number: C2527H) 5. E. coli DH5α cells (NEB, catalog number: C2988J) Reagents 1. Phanta Max Super-Fidelity DNA Polymerase (Vazyme, catalog number: P505) 2. TIANgel Purification

(9) A One-Step Method for Efficient Purification of Functional Cas9 ProteinBio ProtocFebruary 5, 2026Xinzhi Duan, Zhengrong Zhou, Aihua MaoE. coli BL21 (DE3) cells (NEB, catalog number: C2527H) 5. E. coli DH5α cells (NEB, catalog number: C2988J) Reagents 1. Phanta Max Super-Fidelity DNA Polymerase (Vazyme, catalog number: P505) 2. TIANgel Purification

(10) Type I interferon restricts mRNA vaccine efficacy through suppression of antigen uptake in cDCsnpj VaccinesJanuary 5, 2026Tyson A. Lobb, Alexandria Dickson, Wenzheng Guo, Smrithi Beeram, Javier A. Carrero, Yago Dalben, Richard J. DiPaolo, Elise Alspach, Longping V. Tse, Stephen T. Ferrisin vitro mRNA transcription. The plasmid was transformed into DH5α competent E. coli cells (NEB Cat No. C2988J) and plated onto Ampicillin agar plates for resistant colonies to grow. Colonies were harvested and

(11) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce Dempleand methods Competent cells and transformation To prepare DH5α competent cells, DH5α cells (NEB, C2988J) were inoculated into 50 ml of LB broth (0.5% yeast extract, 1% tryptone, and 1% NaCl) and incubated

(12) A noncanonical polyamine from bacteria antagonizes host mitochondrial functionNature CommunicationsNovember 26, 2025Kelsie M. Nauta, Darrick R. Gates, Matthew Weiland, Marco E. Mechan-Llontop, Xiao Wang, Christine Isaguirre, Megan R. Gendjar, Jason Cooper, Sawyer F. Barton, Kim P. Nguyen, et al.the manufacturer’s instructions and maintained in E. coli DH5α competent cells (New England Biolabs, C2988J). Plasmids or purified chromosomal DNA were transformed into B. subtilis strains by natural competency35. All

(13) OLD amputates the anticodon arm of tRNAs during P2–Lambda interferenceNucleic Acids ResearchSeptember 12, 2025Apurva A Govande, Brian D Matibag, Irem Ünlü, Eric J Wolf, Molly R Sargen, Brooke Ramsey, Sangjin Kim, Sophie Helaine, Ivan R Corrêa, Hoong Chuin Limresources Escherichia coli str. K-12 substr. MG1655 New England Biolabs NCBI:txid511145 NEB 5alpha New England Biolabs C2988J MS5106 Laub Lab (MIT) E. coli MFDλpir/pMS749 MS5170 Laub Lab (MIT) E. coli C ΦP2 Δold MS5977 Laub Lab

(14) Large-scale transcriptomic analyses reveal downstream target genes of ZFY1 and ZFY2 transcription factors in male germ cellsCell Death & DifferentiationAugust 27, 2025Hayden Holmlund, Manon Coulée, Yasuhiro Yamauchi, Benazir Yarbabaeva, Muhammetnur Tekayev, Isabella R. Garcia, Olivier U. Feudjio, Alberto de la Iglesia, Lee Larcombe, Peter J. I. Ellis, et al.fusions. The resulting constructs were transformed into NEB 5-alpha competent E. coli cells (NEB, #C2988J) and subsequently purified using a QIAprep Spin Miniprep Kit (Qiagen, Cat. No./ID: 27104). To identify

(15) A high-throughput single-molecule platform to study DNA supercoiling effect on protein-DNA interactionsNucleic Acids ResJune 28, 2025Huijin Lee, Fahad Rashid, Jihee Hwang, James A London, Richard Fishel, James M Berger, Sua Myong, Taekjip Hainactivation at 60°C for 10 min. The mixture was added to competent DH5α Escherichia coli cells (NEB, C2988J) and incubated at 42°C for 30 s for transformation. Transformed cells were cultured in 1 ml of LB broth

(16) Mitochondrial presequences harbor variable strengths to maintain organellar functionbioRxivMay 31, 2025Youmian Yan, Baigalmaa Erdenepurev, Ian Collinson, Natalie M. Niemiligation products were transformed into DH5α competent E. coli cells (New England Biolabs, catalog #C2988J), and plasmids were isolated from transformants. All cloned plasmids were validated by Sanger sequencing. Yeast

(17) Cloning, expression, and characterization of collagen galactosyltransferases from human, sponge, and sea walnutProtein Expression and PurificationMay 1, 2025Jeong Seon Kim, Tingfei Chen, Botao Zhang, Tristin M. Miller, Marisa E. Gilliam, Houfu GuoGLT25Ds Traditional restriction enzyme-based cloning was carried out using the bacterial strain DH5α (NEB, C2988J), while protein expression was performed in E. coli strain BL21(DE3) (Sigma-Aldrich, CMC0014-20X160UL).

(18) Nucleotide-level characterization and improvement of l-arabinose- and l-rhamnose-inducible systems in E. coli using a high-throughput approachNucleic Acids ResApril 10, 2025Nancy M Kim, Danqia Peng, Nicholas R SandovalNEB® 5-alpha Competent E. coli (High Efficiency) (cat. #C2987) and NEB® 5-alpha Competent E. coli (Subcloning Efficiency) (cat. #C2988J) were purchased from New England Biolabs (Ipswich, MA). Electrocompetent ElectroMAX™ DH5α-E Competent

(19) Long-lasting B cell convergence to distinct broadly reactive epitopes following vaccination with chimeric influenza virus hemagglutininsImmunityApril 8, 2025Jenna J. Guthmiller, Linda Yu-Ling Yu-Ling Lan, Lei Li, Yanbin Fu, Sean A. Nelson, Carole Henry, Christopher T. Stamper, Henry A. Utset, Alec W. Freyn, Julianna Han, et al.Stock,N/A A/Michigan/45/2015 H1,Patrick Wilson’s Laboratory Stock,N/A NEB 5-alpha Competent E. coli,NEB,Cat# C2988J Biological samples,, PBMCs from cHA vaccinated subjects,Icahn School of Medicine at Mount Sinai,NCT03300050 PBMCs

(20) Redundant pathways for removal of defective RNA polymerase II complexes at a promoter-proximal pause checkpointMolecular CellDecember 19, 2024Daniel Blears, Jiangman Lou, Nova Fong, Richard Mitter, Ryan M. Sheridan, Dandan He, A. Barbara Dirac-Svejstrup, David Bentley, Jesper Q. Svejstrupstrains,Bacterial and virus strains,Bacterial and virus strains NEB 5-alpha Competent E. coli,NEB,C2988J One Shot BL21 Star (DE3),Thermo Fisher Scientific,C601003 Biological samples,, ,, ,, "Chemicals,

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9/17/2019Transformation of Ligated DNA into Chemically Competent Cells from NEB Atul SinghI would like to recommend these competent cells.