Description
Mouse Embryonic Fibroblasts (MEF) are used to support the growth of undifferentiated mouse or human ES and iPS cells. They provide both a substrate for the ES cells to grow on and secrete many factors necessary for ES cells to maintain pluripotency. MEF cells are isolated from mouse embryos and are used at their early passages. These cells have been mitomycin treated to prevent further cell division prior to cryopreservation. MEF cells from ScienCell Research Laboratories are isolated from E14 mouse embryos. These cells are cryopreserved at P3 after mitomycin C treatment and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume. MEF-mt are characterized by immunofluorescent imaging using antibodies to fibronectin. MEF-mt are negative for mycoplasma, bacteria, yeast and fungi. Recommended Medium It is recommended to use DMEM (Cat. #09221) supplemented with 10% fetal bovine serum (FBS, Cat. #0010, 0025, 0500) for culturing MEF-mt in vitro