Description
Mouse Embryonic Fibroblasts (MEF) are used to support the growth of mouse and human pluripotent stem cells [1]. MEF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. MEF are isolated from mouse embryos and used at early passages [2]. To serve as feeder cells, MEF must be treated with mitomycin C or by irradiation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells. MEF-mt (Cat. No. M7570-mt) from ScienCell Research Laboratories are isolated from E13 embryos of CF1 mice and have been treated with mitomycin C to prevent further cell division. They are cryopreserved at passage 3 and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume. MEF-mt are characterized by immunofluorescence with antibodies specific to fibronectin. MEF-mt are negative for mycoplasma, bacteria, yeast and fungi