Description
M2 macrophages are derived from purified CD14+ monocytes. CD14+ monocytes were positively selected via immunomagnetic beads and were cultured in the presence of M-CSF for 10 days. The M2 phenotyping was confirmed by morphology and the expression of specific biomarkers which monitored by flow cytometry. The cells were harvested after 10 days of in vitro culture.
Macrophages are a type of white blood cell found in all tissues patrolling for potential pathogens. Macrophages are key players in the immune response, basically ridding the body of worn-out cells, foreign substances, microbes, and cancer cells and alerting the immune system to the presence of invaders. Because macrophages possess the ability to eliminate pathogens and recruit and instruct other immune cells, they play a central role in protecting the body and contribute to the pathogenesis of inflammatory and degenerative diseases. Macrophages can modify themselves and change their functional phenotype depending on the environmental. HemaCare supplies both types of macrophages. M2 macrophages decrease inflammation and encourage tissue repair