Primary Gingival Fibroblast; Normal, Human, Adult (HGF) from ATCC

Product Specs
ItemPrimary Gingival Fibroblast; Normal, Human, Adult (HGF)
CompanyATCC
Price Supplier Page View Company Product Page
Catalog NumberPCS-201-018
ApplicationsHuman gingival fibroblasts (hGF) could potentially be an alternative source of mesenchymal stem cells (MSC) for regenerative medicine studies as they share similar morphology, CD markers, and differentiation lineage
SpeciesHomo sapiens
TypeCell Biology
TissueGingiva
Cell Typefibroblast
Age60 years

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

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Website: http://www.atcc.org

(1) Benzyl-Naphthoquinones as Selective Anticancer Agents for Oral Squamous Cell Carcinoma via Apoptosis InductionBiomedicinesMarch 26, 2026Antonio Mendonça Marconi-Nicolau, Rebeca Gripp de Sá, Caroline Reis Santiago Paschoal, Lethícia Andrade de Almeida, Gabriel Ouverney, Ana Caroline dos Santos-Diniz, Anamel Blaudt Meira, João Pedro da Costa Faria Brunhosa, Luiz Carlos da Silva Pinheiro, Paula Alvarez Abreu, et al.CRL-2539, respectively). Primary normal human gingival fibroblasts (HGFs) were obtained from ATCC (PCS-201-018). SCCs were maintained in 1:1 DMEM/F12 (Dulbecco’s modified Eagle medium (DMEM) and Ham’s F12 medium

(2) Effects of Thermal Aging on Optical, Surface, Mechanical, and Biological Properties of CAD/CAM Polymer-Based Dental MaterialsPolymers (Basel)March 20, 2026Gül Ateş, Mustafa Gungormusthe obtained extracts were used immediately after preparation. Human gingival fibroblast (HGF) cells (PCS-201-018™, ATCC, Manassas, VA, USA) were used for cytotoxicity evaluation. Cells (passage 7) were thawed at 37

(3) Novel Insights on Benzo[b]thiophene Analogues for MAO-B Inhibition and Neuroprotection: Design, Synthesis, Molecular Modelling Studies and Biological ActivityAntioxidants (Basel)March 10, 2026Francesca Arrighi, Emanuela Berrino, Paolo Guglielmi, Simone Carradori, Guya Diletta Marconi, Jacopo Pizzicannella, Simone Guarnieri, Tiziano Tuccinardi, Giulio Poli, Federico Pepi, et al.−Ki [46]. 2.3.3. Cell Culture The human gingival fibroblast (hGF) cell line was acquired from ATCC (PCS-201-018 ATCC, Manassas, VA, USA) and cultured with Fibroblast Basal Medium (485 mL; ATCC PCS-201-030) included

(4) Targeted photodynamic therapy for multispecies dental biofilms using liposome encapsulated p-ToluquinoneChemical Engineering JournalFebruary 15, 2026Chen Liang, Yongli Li, Zuan-Tao Lin, Zhilong Wang, Ya He, Yan Dong, Zihan Ran, Sophia Zhang, Ying Zhao, Songling Wang, et al.relevant groups was measured by flow cytometry. Cytotoxicity Human gingival fibroblasts (HGF; ATCC PCS-201–018) were cultured in DMEM in 48-well plates (Corning). Cells were divided into treatment groups: sham control,

(5) Targeted Apoptosis Induction in Oral Squamous Cell Carcinoma by Goniothalamus umbrosus : A Pathway Through Bax, Bcl-2, and Caspase 3European Journal of DentistryDecember 18, 2025Nuraini Che Aziz, Basma Ezzat Mustafa Alahmad, Muhanad Ali Kashmoola, Widya Lestari, Nik Mohd Mazuan Nik Mohd Rosdy, Khairani Idah Mokhtarprimarily excised from the gingiva of the donor (American Type Culture Collection [ATCC]; catalog no.: PCS-201-018). Both cell lines were cultured in DMEM media (ATCC catalog no.: 30-2006) supplemented with 10% FBS

(6) Coumarin-1,2,3-Triazole Conjugates as Molecular Scaffolds for the Selective Induction of ROS-Driven Apoptosis in Cancer Cells in the Development of Pyruvate Kinase M2 InhibitorsACS OmegaDecember 9, 2025Gabriel Ouverney, Amanda de Andrade Borges, Acácio Souza da Silva, Caroline Reis Santiago Paschoal, Paula Alvarez Abreu, Analice Gonçalves Rodrigues da Cruz, Mateus de Freitas Brito, Lucas Silva Abreu, Vitor Francisco Ferreira, Fernando de Carvalho da Silva, et al.and CRL-6475, respectively). Primary normal human gingival fibroblasts (HGF) were obtained from ATCC (PCS-201-018). SCCs were maintained in 1:1 DMEM/F12 (Dulbecco’s modified Eagle medium and Ham’s F12 medium; Gibco

(7) 3D Printing of Shape Memory Resin for Orthodontic Aligners with Green Synthesized Antimicrobial ZnO Nanoparticles Coatings: Toward Bioactive DevicesBioengineering (Basel)November 1, 2025Airy Teramoto-lida, Rafael Álvarez-Chimal, Lorena Reyes-Carmona, Marco Antonio Álvarez-Pérez, Amaury Pozos-Guillen, Febe Carolina Vázquez-Vázquezguidelines. 2.6. Biocompatibility Evaluation 2.6.1. Cell Culture Human gingival fibroblasts (hGFs; ATCC PCS-201-018) were used for the biocompatibility assay. Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM)

(8) Effects of silica particle size on the mechanical, surface and printing-trueness properties of 3D-printing dental resinSci RepOctober 13, 2025Di Chen, Gan Jin, Zhihao Zhang, Yifan Ma, Yunqi Liu, Jung-Hwa Lim, Reuben Kim, Jong-Eun Kimcalculated. Relative cell viability The cytotoxicity of the specimens toward human gingival fibroblasts (HGFs, PCS-201-018, ATCC) was evaluated with a WST-8 cell-proliferation assay following established method62. Frozen HGFs

(9) Mechanical and Antimicrobial Evaluation of Chitosan-Coated Elastomeric Orthodontic ModulesDentistry JournalSeptember 29, 2025Lucía Gabriela Beltrán-Novelo, Fernando Javier Aguilar-Pérez, Myriam Angélica De La Garza-Ramos, Arturo Abraham Cienfuegos-Sarmiento, José Rubén Herrera-Atoche, Martha Gabriela Chuc-Gamboa, Jacqueline Adelina Rodríguez-Chávez, Juan Valerio Cauich-Rodríguezmedium (DMEM) and incubation of the plates for 4 h under standard conditions) was performed using ATCC PCS-201-018 gingival fibroblasts [31]. A density of 10,000 cells per well was placed in a volume of 100 µL per well.

(10) Phytochemical characterization, anticancer potential, and nanoemulsion-based delivery of Chiliadenus montanusPLOS OneSeptember 26, 2025Nour Aboalhaija, Hala Abulawi, Rania Hamed, Mohammad Alwahsh, Fatma Afifi, Heba Syaj, Elham Abusharieh, Ismail AbazaUSA). A549 (CCL-185) and H1299 (CRL-5803) cells were cultured in RPMI-1640 medium, while fibroblasts (PCS-201–018) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM). Both media were supplemented with 10% fetal

(11) Mechanistic Insights into the Anticancer Action of Novel 2-Hydroxy-1,4-naphthoquinone Thiol DerivativesACS OmegaSeptember 16, 2025Thaís Barreto Santos, Alex de Souza Cruz Lopes Canuto, João Francisco Blaudt Virgilio de Carvalho Meira, Ana Caroline Santos-Diniz, Rafaella Machado de Assis Cabral Ribeiro, Caroline Reis Santiago Paschoal, Vitor Won-Held Rabelo, Paula Alvarez Abreu, Vitor Francisco Ferreira, David Rodrigues da Rocha, et al.(liver cancer; HB-8065), in addition to primary normal human gingival fibroblasts obtained from ATCC (PCS-201-018), were maintained in DMEM supplemented with 10% (v/v) of FBS and were used in a maximum of six passages.

(12) From an Antimicrobial Agent to a constituent of 3D Printed Heterogenous Scaffolds Stimulating Bone Characteristics: An In-vitro and Animal model evaluationRegen TherAugust 12, 2025Shahad Ahmed Daood, Martha Then Xin Yi, Nicole Wen Ce Mun, Sharjeel Ilyas, Lee Yin Shien, Oh Jia En, Syed Saad Bin Qasim, Yichen Dai, Galvinderjeet Kaur Grewal, Ng Mei Liit, et al.sputter-coating with gold for 10 s at a vacuum pressure of 80 mTorr. The human gingival fibroblasts (ATCC; HGF, PCS-201−018, ATCC, USA) were cultured at 37 ± 2 °C in a humidified environment (5 % CO2). Upon attaining 60–80 %

(13) In Vitro Evaluation of the Antibacterial Properties and Cellular Response of Liquid-Leukocyte Platelet-Rich Fibrin Products on Barrier Membranes: A Pilot StudyDentistry JournalMay 22, 2025Nichol Chun Wai Tsang, Aneesha Acharya, Georgios Pelekos(HGF) Cell-Culture Experiments 4.4.1. Cells and Culture A human gingival fibroblast 1 cell line (PCS-201-018, ATCC® CRL-2014™, Manassas, VA, USA) (HGF-1) was procured from ATCC® and cultures from passage 4–6 were

(14) Time-Dependent Changes in Effects of Butyrate on Human Gingival FibroblastsClin Exp Dent ResApril 24, 2025Haruki Otani, Jumpei Washio, Aoi Kunitomi, Satoko Sato, Yuki Abiko, Shiori Sasaki, Kazumasa Ohashi, Satoru Yamada, Nobuhiro TakahashiMaterials and Methods 2.1 Cell Lines and Culture Conditions A human gingival fibroblast cell line (HGF) (PCS‐201‐018, ATCC, Virginia, USA) was used. HGF were cultured in Dulbecco's Modified Eagle's medium DMEM (10‐103‐CVR,

(15) Unravelling the Programmed Inflammation and Tissue Repair by a Multipotential Antimicrobial K21 SilaneInternational Dental JournalApril 1, 2025Umer Daood, Muhammad Sharjeel Ilyas, Sehar Bashir, Neelofar Yousuf, Maryam Rashid, Kanwardeep Kaur, Ranjeet Ajit Bapat, Mohammed Nadeem Bijle, Malikarjuna Rao Pichika, Kit-Kay Mak, et al.fibroblasts (PGF) were isolated from the jaw of a 65-year-old Caucasian male obtained from ATCC (HGF, PCS-201–018, ATCC, USA). Media and culture conditions To initiate an experiment, a working frozen culture was

(16) In vitro evaluation of cytotoxicity of fixed functional appliancesBMC Oral HealthMarch 13, 2025Alper Balat, Hakan Gürcan Gürel, Nazlı Ece Orduerisamples in each group was compared with control groups of a human gingival fibroblast (HGF) cell line (PCS-201–018, ATCC; Manassas, Virginia, USA). Sample size calculation The sample size was calculated as n = 70

(17) Phytochemical Profile and Anticancer Potential of Helichrysum arenarium Extracts on Glioblastoma, Bladder Cancer, and Breast Cancer CellsPharmaceuticals (Basel)January 22, 2025Ihsan Nalkiran, Hatice Sevim Nalkiranepithelial cells), kindly provided by Prof. Saliha Eksi (Recep Tayyip Erdogan University, Rize, Turkiye); and hGF (PCS-201-018, human gingival fibroblast cells), purchased from ATCC. All cells were cultured in cell media containing

(18) Evaluation of the anti-inflammatory, antioxidant and wound healing effects of pterostilbene in human gingival fibroblasts in vitroOdontologyJanuary 21, 2025Mukaddes Yerebakan, Gulay Tuter, Emin Umit Bagriacik, Nihan Oruklu, Tugce GuldururE-21071282–050.99–462,173). Cell culture Human primary gingival fibroblast cells were purchased from ATCC (ATCC® PCS-201–018, Virginia, USA). The cells were thawed at 37 °C. The cells were centrifuged fibroblast growth kit (ATCC®

(19) Effect of ozone oil and non-surgical periodontal treatment in patients with type 2 diabetes. In-vivo and in-vitro studies with fibroblasts and Candida albicansJournal of Applied Oral ScienceJanuary 20, 2025Raquel Alves do do CARMO, Ana Carolina Organista CÖRNER, Eduardo Ferreira MARTINS, Gabriel OUVERNEY, Julio Cesar THURLER JÚNIOR, Bruno Kaufmann ROBBS, Vinicius D’Avila Bitencourt PASCOAL, Elisa ESPOSITO, Luciane Portas CAPELO, Gabriela Alessandra da Cruz Galhardo CAMARGOBrazil). Cell viability (Cytotoxicity) assay Primary normal human gingival fibroblast was obtained from ATCC (PCS-201-018) and maintained in DMEM (Dulbecco’s modified Eagle’s medium) with high (4500mg/L, Catalog number: 12800017;

(20) Mannich Base Derived from Lawsone Inhibits PKM2 and Induces Neoplastic Cell DeathBiomedicinesDecember 21, 2024Lucas Rubini-Dias, Tácio V. A. Fernandes, Michele P. de Souza, Déborah Hottz, Afonso T. Arruda, Amanda de A. Borges, Gabriel Ouverney, Fernando de C. da Silva, Luana da S. M. Forezi, Gabriel Limaverde-Sousa, et al.respectively). Normal human primary gingival fibroblasts (HGF) were obtained by ATCC (Masassas, VA, USA) (PCS201 018). The OSCC line was maintained in 1:1 DMEM/F12 (Dulbecco’s Modified Eagle Medium and Ham’s F12 medium;

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Primary Gingival Fibroblast; Normal, Human, Adult (HGF) from ATCC

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Primary Gingival Fibroblast; Normal, Human, Adult (HGF) from ATCC

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