2D12 from ATCC

Product Specs
Item2D12
CompanyATCC
Price Supplier Page View Company Product Page
Catalog NumberCRL-1689
SpeciesMus musculus B cell Mus musculus myeloma
TypeCell Biology
Cell Typehybridoma b lymphocyte
ApplicationsTested and found negative for ectromelia virus (mousepox)

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Two mutations in NS2B are responsible for attenuation of the yellow fever virus (YFV) vaccine strain 17DPLOS PathogensJuly 31, 2025Xueer Qiu, Adam L. Baileyantibody was prepared from clarified and filtered supernatant collected from mouse hybridoma cells (ATCC, CRL-1689). After washing, cells were incubated with an HRP-conjugated secondary antibody (1:1000, Jackson ImmunoResearch)

(2) The neonatal Fc receptor (FcRn) is a pan-arterivirus receptorNature communicationsAugust 7, 2024Shaw TM, Huey D, Mousa-Makky M, Compaleo J, Nennig K, Shah AP, Jiang F, Qiu X, Klipsic D, Rowland RRR, et al.assays YFV focus-forming assay was performed as described previously59, using 2D12 hybridoma (ATCC, CRL-1689) supernatant (1:1000) as primary antibody. CRISPR-knockout screen in MA-104 cells 2.4 × 107 MA-104

(3) Broad-spectrum activity against mosquito-borne flaviviruses achieved by a targeted protein degradation mechanismNature communicationsJune 19, 2024Liu HY, Li Z, Reindl T, He Z, Qiu X, Golden RP, Donovan KA, Bailey A, Fischer ES, Zhang T, et al.temperature, washed with DPBS, and then incubated overnight with monoclonal antibody 2D12 mouse anti-YFV E (CRL-1689, ATCC) at a ratio of 1:800 at 4 °C and washed with DPBS. After washing, the cells were incubated with

(4) A Photoactivable Natural Product with Broad Antiviral Activity against Enveloped Viruses, Including Highly Pathogenic CoronavirusesAntimicrobial Agents and ChemotherapyFebruary 15, 2022Thomas Meunier et al.mg/ml. Antibodies. Mouse anti-HCV E1 monoclonal antibody (MAb) A4 (56) and mouse anti-YFV E MAb 2D12 (anti-E; ATCC CRL-1689) were produced in vitro by using a MiniPerm apparatus (Heraeus). Mouse anti-double-stranded RNA MAb

(5) Pre-existing yellow fever immunity impairs and modulates the antibody response to tick-borne encephalitis vaccinationnpj VaccinesDecember 1, 2019Victoria Bradt, Stefan Malafa, Amrei von Braun, Johanna Jarmer, Georgios Tsouchnikas, Iris Medits, Kerstin Wanke, Urs Karrer, Karin Stiasny, Franz X. Heinzdetected using a YF virus-specific mouse monoclonal antibody (1 µg/ml 2D12; affinity-purified from ATCC CRL-1689 hybridoma cells) in combination with an Alexa Fluor 488 labeled rabbit anti-mouse IgG (Invitrogen, catalog

(6) Effects of Regulatory T Cell Depletion on NK Cell Responses against Listeria monocytogenes in Feline Immunodeficiency Virus Infected CatsVirusesOctober 24, 2019Rita D. Simões, Alora LaVoy, Gregg A. Deanage-matched female SPF cats. Mouse anti-feline CD25 (9F23) [16] and mouse anti-yellow fever antigen (YFA; CRL-1689, ATCC, Manassas, VA, USA) monoclonal antibodies (mAb) were purified and certified mycoplasma and endotoxin-free

(7) Dehydrojuncusol, a Natural Phenanthrene Compound Extracted from Juncus maritimus , Is a New Inhibitor of Hepatitis C Virus RNA ReplicationJournal of VirologyMay 15, 2019Marie-Emmanuelle Sahuc et al.MO). Antibodies. The anti-E1 monoclonal antibody (A4) (38), anti-YFV envelope protein 2D12 antibody (ATCC CRL-1689), and anti-BVDV NS3 protein OSC-23 antibody (39) were produced in vitro by using the miniPerm apparatus

(8) Vaccine and Wild-Type Strains of Yellow Fever Virus Engage Distinct Entry Mechanisms and Differentially Stimulate Antiviral Immune ResponsesmBioMarch 2, 2016Maria Dolores Fernandez-Garcia et al.previously described (64). Mouse anti-E protein 2D12 were produced from the hydridoma cell line ATCC CRL-1689. Mouse anti-E 4G2 and ascites from mice inoculated with the French neurotropic (FNV) strain of YFV were

(9) New Insights into the Understanding of Hepatitis C Virus Entry and Cell-to-Cell Transmission by Using the Ionophore Monensin AJournal of VirologyAugust 15, 2015Lucie Fénéant et al.France). Antibodies. Mouse anti-HCV E1 (A4) (43) and anti-yellow fever virus (YFV) envelope (2D12; ATCC CRL-1689) (44) monoclonal antibodies (MAbs) were produced in vitro by using a MiniPerm apparatus (Heraeus) as

(10) The antimalarial ferroquine is an inhibitor of hepatitis C virusHepatologyJuly 1, 2013Thibaut Vausselin et al.McKeating, University of Birmingham, UK)(17),17 anti–yellow fever virus (YFV) envelope protein (2D12) (ATCC CRL‐1689), anti–bovine viral diarrhea virus (BVDV) NS3 protein (Osc‐23),18 anti‐NS5A antibody (Ab) (AUSTRAL Biologicals,

(11) Partial regulatory T cell depletion prior to acute feline immunodeficiency virus infection does not alter disease pathogenesisPloS oneFebruary 25, 2011Mikkelsen SR, Long JM, Zhang L, Galemore ER, VandeWoude S, Dean GA.National Institutes of Health in Tokyo, Japan, and mouse anti-yellow fever antigen (YFA) hybridoma (CRL-1689; ATCC, Manassas, VA) were grown in serum-free medium. Antibody was purified and certified endotoxin-

(12) In vivo depletion of CD4(+)CD25(hi) regulatory T cells is associated with improved antiviral responses in cats chronically infected with feline immunodeficiency virusVirologyAugust 1, 2010Mikkelsen SR, Reckling SK, Egan EA, Dean GA.National Institutes of Health in Tokyo, Japan, and mouse anti-yellow fever antigen (YFA) hybridoma (CRL-1689; ATCC, Manassas, VA) were expanded, purified, and certified endotoxin- and mycoplasma-free by Leinco,

(13) In vivo depletion of CD4+CD25+ regulatory T cells in catsJournal of Immunological MethodsJanuary 1, 2008S. Rochelle Smithberg, Jonathan E. Fogle, Angela M. Mexas, Stacie K. Reckling, Susan M. Lankford, Mary B. Tompkins, Gregg A. Deanof Health, Tokyo, Japan) (Ohno et al., 1992). Mouse anti-yellow fever antigen (YFA) mAb hybridoma (CRL-1689, ATCC, Manassas, VA) was also obtained to produce an IgG2a kappa light chain antibody as an isotype

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