Description
The Cas9 Expressing iPS Cell Pool constitutively expresses Cas9 (Streptococcus pyogenes CRISPR associated protein 9) under the control of an EF1A promoter, introduced into PBMC-derived human iPS cells via lentiviral transduction (Cas9 Lentivirus, BPS Bioscience #78066). Cas9 Expressing iPS cells can be transduced with single-guide RNA (sgRNA) targeting gene(s) of interest to generate genetically engineered cell pools or cell lines