Description
Activated primary rat portal fibroblasts (PF) were immortalized with the SV40 large T antigen mammalian expression vector to generate the Immortalized Rat Portal Myofibroblasts (RGF). RGF cells are negative for hepatic stellate cell markers, and like the primary cells, they demonstrate inhibition of cholangiocyte proliferation in co-culture conditions. The easily transfectable nature of these cells make them an ideal research tool for studying both the function of myofibroblasts and their contribution to liver fibrosis progression