Description
The Immortalized Mouse OP9-Derived Stromal Cells (OP9-K) display classic adipocyte morphology: rounded shape, lipid accumulation, and coalescence of lipids into a large droplet. OP9-K cells are able to differentiate via known adipogenic pathways, involving the transcriptional activation and repression of common adipose markers Plin1, Gata2, C/Ebpa and C/Ebpb and biological pathways, such as lipid metabolism, PPARc signaling, and osteogenesis. The characteristics of the OP9-K cell line prove it as a suitable adipocyte model system. The OP9-K cell line is an important tool in understanding adipose biology with its rapid, robust differentiation and efficient transfectability in adipogenesis. The cells also have the potential to efficiently test obesity therapeutics and may be suitable for high-throughput RNAi screens of adipogenesis