Description
Cynomolgus Monkey Primary Skeletal Muscle Cells are isolated from Cynomolgus Monkey skeletal muscle tissue. Cynomolgus Monkey Primary Skeletal Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray Culture Complete Growth Medium generally for 3-7days. Prior to shipping, cells at passage 1 are detached from the culture flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. The cells are characterized by immunofluorescent method with antibodies to α-smooth muscle actin. Cynomolgus Monkey Primary Skeletal Muscle Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments (or cells may be expanded for 2 passages at a split ratio of 1:2) under the cell culture conditions specified by Cretive Bioarray. Repeated freezing and thawing of cells is not recommended