Description
T-cells are functionally divided into helper T-cells, which are CD4+ CD8- ("T4"), and cytotoxic T-cells, which are CD8+ CD4- ("T8").
T cells can also be divided based on differentiation status into 4 subsets:
1. Naïve ("Tn ", containing Tscm), which are CD27+ CCR7+ CD45RO
2. Central-memory ("Tcm"), which are CD27+ CCR7+ CD45RO+
3. Effector-memory ("Tem"), which are CD27- CCR7- CD45RO+
4. Effector ("Teff"), which are CD27- CCR7- CD45RO-
Human PBMC are separated into 4 subsets using antibodies and magnetic beads. The subsets were: CD4+CD8- T cells (T4), CD8+CD4- T cells (T8), CD4+ central memory T cells (Tcm) and CD4+ effector-memory T cells (Tem). Immediately after isolation, the subsets and the PBMC were analyzed by flow
cytometry for the composition of the subsets