Viral Production Cells 2.0 from Thermo Fisher Scientific

Description

  • Gibco Viral Production Cells 2.0 are a clonal cell line derived from the HEK293F parental cell line
  • They are a core component of the AAV-MAX Helper-Free AAV Production System
  • The cells have been adapted to the chemically defined Viral Production Medium and are maintained in suspension culture in that medium
  • Viral Production Cells 2.0 are highly transfectable using the AAV-MAX Transfection Kit for adeno-associated virus (AAV) production
  • Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Viral Production Medium and 10% DMSO
  • Cells should be thawed directly into Viral Production Medium supplemented with 4 mM GlutaMAX Supplement (Cat. No. 35050038) for optimal performance

Features of the Viral Production Cells 2.0 include:
  • Clonal, 293F-derived, high-producer cell line
  • Absence of SV40 large T antigen
  • Optimized for high-density, suspension culture (>12 million cells/mL) in chemically defined medium
  • Enable production of high AAV titers, greater than 5 x 1010 vg/mL (unpurified)
  • Robust scalability and passage stability