Description
Introduction: Introduction: S1PR5 is a G-protein-coupled receptor which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P).S1PR5 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR5-5).They areexpressed in a wide variety of tissues, with each subtype exhibiting a different cell specificity, although they are found attheir highest density on leuk degree Cytes. S1PR5 may play a regulatory role in the transformation of radial glial cells intoastr degree Cytes and may affect proliferative activity of these cells.
Description: Human S1PR5 ACTOneTM is a HEK-293 CNG cell line that expresses recombinant human S1PR5. HEK-293 CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levelsand consequently result in ion flux (often detectable by calcium-responsive dye) and cell membranedepolarization which can be easily measured with fluorescent Membrane Potential Dye. The assayallows both end-point and kinetic measurement of intracellular cAMP changes with a FDSS, FLIPR, or a fluorescencemicroplate reader.
This cell line is a HEK-293-CNG cell line that expresses a recombinant human S1PR5. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization