Description
Introduction: Introduction: S1PR2 is a G-protein-coupled receptor which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P). S1PR2 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR2-5). They areexpressed in a wide variety of tissues, with each subtype exhibiting a different cell specificity, although they are found attheir highest density on leuk degree Cytes. S1PR2 protein participates in sphingosine 1-phosphate-induced cell proliferation,survival, and transcriptional activation.
Description: Human S1PR2 ACTOneTM is a HEK-293 CNG cell line that expresses recombinant human S1PR2. HEK-293 CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux (often detectable by calcium-responsive dye) and cell membrane depolarization which can be easily measured with fluorescent Membrane Potential Dye. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FDSS, FLIPR, or a fluorescence microplate reader.
This cell line is a HEK-293-CNG cell line that expresses a recombinant human S1PR2. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization