Description
The LS147T cell line is a variant of LS-180, both of which are derived from a Duke's type B adenocarcinoma of the colon in a 58-year-old White female patient. The original LS-180 line was established by culturing the minced tumor tissue for 10 months. LS-147T, along with its parent line, is notable for its expression of multiple oncogenes including myc, myb, ras, and fos, while being negative for others like sis, abl, and ros. This line also expresses high levels of carcinoembryonic antigen (CEA), interleukin 6 (IL-6), and interleukin 10 (IL-10), which are important markers and potential targets in colorectal cancer research.
These cells exhibit several key characteristics of colonic epithelial cells, including abundant microvilli and intracytoplasmic mucin vacuoles, which are features typically associated with secretory cells in the colonic mucosa. Electron microscopy studies have confirmed these structural details, further supporting their origin and differentiation status. Importantly, LS-147T cells have been shown to be tumorigenic in immunodeprived mice, consistently producing tumors when inoculated subcutaneously at high cell densities, thus affirming their malignant potential.
Moreover, the LS-147T cell line is particularly valuable in studies focusing on the molecular and immunological aspects of colorectal cancer. It has been reported that this line is easier to subculture compared to its parent line, LS-180, making it a more practical choice for long-term studies. The robust production of CEA by these cells, which is significantly higher than that of other established lines like HT-29, makes LS-147T a critical model for understanding tumor marker dynamics and exploring targeted therapies in colorectal cancer