Description
Diabetic Mouse Lymphatic Endothelial Cells from Creative Bioarray are isolated from the lymph nodes of Diabetic (db/db) mice. Diabetic Mouse Lymphatic Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Creative Bioarray’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×10^6cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with LYVE-1 antibody, following the application of magnetic beads pre-coated with secondary antibody